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A flexible and efficient template format for circular consensus sequencing and SNP detection
A novel template design for single-molecule sequencing is introduced, a structure we refer to as a SMRTbell™ template. This structure consists of a double-stranded portion, containing the insert of interest, and a single-stranded hairpin loop on either end, which provides a site for primer binding....
| Autores principales: | , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2926623/ https://www.ncbi.nlm.nih.gov/pubmed/20571086 http://dx.doi.org/10.1093/nar/gkq543 |