A dumbbell probe-mediated rolling circle amplification strategy for highly sensitive microRNA detection

We herein report the design of a dumbbell-shaped DNA probe that integrates target-binding, amplification and signaling within one multifunctional design. The dumbbell probe can initiate rolling circle amplification (D-RCA) in the presence of specific microRNA (miRNA) targets. This D-RCA-based miRNA...

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Detalles Bibliográficos
Autores principales: Zhou, Yuntao, Huang, Qing, Gao, Jimin, Lu, Jianxin, Shen, Xizhong, Fan, Chunhai
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2926626/
https://www.ncbi.nlm.nih.gov/pubmed/20547593
http://dx.doi.org/10.1093/nar/gkq556
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author Zhou, Yuntao
Huang, Qing
Gao, Jimin
Lu, Jianxin
Shen, Xizhong
Fan, Chunhai
author_facet Zhou, Yuntao
Huang, Qing
Gao, Jimin
Lu, Jianxin
Shen, Xizhong
Fan, Chunhai
author_sort Zhou, Yuntao
collection PubMed
description We herein report the design of a dumbbell-shaped DNA probe that integrates target-binding, amplification and signaling within one multifunctional design. The dumbbell probe can initiate rolling circle amplification (D-RCA) in the presence of specific microRNA (miRNA) targets. This D-RCA-based miRNA strategy allows quantification of miRNA with very low quantity of RNA samples. The femtomolar sensitivity of D-RCA compares favorably with other existing technologies. More significantly, the dynamic range of D-RCA is extremely large, covering eight orders of magnitude. We also demonstrate miRNA quantification with this highly sensitive and inexpensive D-RCA strategy in clinical samples.
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spelling pubmed-29266262010-08-30 A dumbbell probe-mediated rolling circle amplification strategy for highly sensitive microRNA detection Zhou, Yuntao Huang, Qing Gao, Jimin Lu, Jianxin Shen, Xizhong Fan, Chunhai Nucleic Acids Res Methods Online We herein report the design of a dumbbell-shaped DNA probe that integrates target-binding, amplification and signaling within one multifunctional design. The dumbbell probe can initiate rolling circle amplification (D-RCA) in the presence of specific microRNA (miRNA) targets. This D-RCA-based miRNA strategy allows quantification of miRNA with very low quantity of RNA samples. The femtomolar sensitivity of D-RCA compares favorably with other existing technologies. More significantly, the dynamic range of D-RCA is extremely large, covering eight orders of magnitude. We also demonstrate miRNA quantification with this highly sensitive and inexpensive D-RCA strategy in clinical samples. Oxford University Press 2010-08 2010-06-14 /pmc/articles/PMC2926626/ /pubmed/20547593 http://dx.doi.org/10.1093/nar/gkq556 Text en © The Author(s) 2010. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/2.5 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methods Online
Zhou, Yuntao
Huang, Qing
Gao, Jimin
Lu, Jianxin
Shen, Xizhong
Fan, Chunhai
A dumbbell probe-mediated rolling circle amplification strategy for highly sensitive microRNA detection
title A dumbbell probe-mediated rolling circle amplification strategy for highly sensitive microRNA detection
title_full A dumbbell probe-mediated rolling circle amplification strategy for highly sensitive microRNA detection
title_fullStr A dumbbell probe-mediated rolling circle amplification strategy for highly sensitive microRNA detection
title_full_unstemmed A dumbbell probe-mediated rolling circle amplification strategy for highly sensitive microRNA detection
title_short A dumbbell probe-mediated rolling circle amplification strategy for highly sensitive microRNA detection
title_sort dumbbell probe-mediated rolling circle amplification strategy for highly sensitive microrna detection
topic Methods Online
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2926626/
https://www.ncbi.nlm.nih.gov/pubmed/20547593
http://dx.doi.org/10.1093/nar/gkq556
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