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The effects of rapamycin on lens epithelial cell proliferation, migration, and matrix formation: An in vitro study
PURPOSE: The objective of the present study was to investigate the efficacy of rapamycin on rabbit lens epithelial cell proliferation, migration, and secrcetion of extracellular matrix fibronectin (Fn). METHODS: Rabbit lens epithelium cells (rLECs) were isolated from 1 month old rabbit. rLECs were e...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Molecular Vision
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2927374/ https://www.ncbi.nlm.nih.gov/pubmed/20806034 |
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author | Liu, Hongling Feng, Guangzhong Wu, Lan Fu, Shaoying Liu, Ping Yang, Wei Zhang, Xiaomei |
author_facet | Liu, Hongling Feng, Guangzhong Wu, Lan Fu, Shaoying Liu, Ping Yang, Wei Zhang, Xiaomei |
author_sort | Liu, Hongling |
collection | PubMed |
description | PURPOSE: The objective of the present study was to investigate the efficacy of rapamycin on rabbit lens epithelial cell proliferation, migration, and secrcetion of extracellular matrix fibronectin (Fn). METHODS: Rabbit lens epithelium cells (rLECs) were isolated from 1 month old rabbit. rLECs were either cultured for 24, 48, or 72 h with different doses of rapamycin (0.1, 1, and 10 ng/ml). The proliferation kinetics, proliferating cell nuclear antigen (PCNA) expression, and basic fibroblast growth factor (bFGF)-induced migration of rLEC was determined by methyl thiazol tetrazolium (MTT) assay, western blotting and transwell chamber assay, respectively. The effect of rapamycin on the synthesis of Fn was examined via immunofluorescence. RESULTS: Rapamycin significantly inhibited rLEC proliferation and PCNA protein expression when administered doses and time periods except for 0.1 ng/ml for 24 h. bFGF-induced migration rLECs was inhibited by pretreatment with rapamycin for 48 h. Extracellular matrix Fn formation of rLECs was also reduced by rapamycin. CONCLUSIONS: In our study, rapamycin strongly inhibited rLEC proliferation, bFGF-induced migration, and extracellular matrix Fn formation. Thus, rapamycin may have a potential inhibition of posterior capsule opacificatin (PCO) and needs further study. |
format | Text |
id | pubmed-2927374 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Molecular Vision |
record_format | MEDLINE/PubMed |
spelling | pubmed-29273742010-08-30 The effects of rapamycin on lens epithelial cell proliferation, migration, and matrix formation: An in vitro study Liu, Hongling Feng, Guangzhong Wu, Lan Fu, Shaoying Liu, Ping Yang, Wei Zhang, Xiaomei Mol Vis Research Article PURPOSE: The objective of the present study was to investigate the efficacy of rapamycin on rabbit lens epithelial cell proliferation, migration, and secrcetion of extracellular matrix fibronectin (Fn). METHODS: Rabbit lens epithelium cells (rLECs) were isolated from 1 month old rabbit. rLECs were either cultured for 24, 48, or 72 h with different doses of rapamycin (0.1, 1, and 10 ng/ml). The proliferation kinetics, proliferating cell nuclear antigen (PCNA) expression, and basic fibroblast growth factor (bFGF)-induced migration of rLEC was determined by methyl thiazol tetrazolium (MTT) assay, western blotting and transwell chamber assay, respectively. The effect of rapamycin on the synthesis of Fn was examined via immunofluorescence. RESULTS: Rapamycin significantly inhibited rLEC proliferation and PCNA protein expression when administered doses and time periods except for 0.1 ng/ml for 24 h. bFGF-induced migration rLECs was inhibited by pretreatment with rapamycin for 48 h. Extracellular matrix Fn formation of rLECs was also reduced by rapamycin. CONCLUSIONS: In our study, rapamycin strongly inhibited rLEC proliferation, bFGF-induced migration, and extracellular matrix Fn formation. Thus, rapamycin may have a potential inhibition of posterior capsule opacificatin (PCO) and needs further study. Molecular Vision 2010-08-16 /pmc/articles/PMC2927374/ /pubmed/20806034 Text en Copyright © 2010 Molecular Vision. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Liu, Hongling Feng, Guangzhong Wu, Lan Fu, Shaoying Liu, Ping Yang, Wei Zhang, Xiaomei The effects of rapamycin on lens epithelial cell proliferation, migration, and matrix formation: An in vitro study |
title | The effects of rapamycin on lens epithelial cell proliferation, migration, and matrix formation: An in vitro study |
title_full | The effects of rapamycin on lens epithelial cell proliferation, migration, and matrix formation: An in vitro study |
title_fullStr | The effects of rapamycin on lens epithelial cell proliferation, migration, and matrix formation: An in vitro study |
title_full_unstemmed | The effects of rapamycin on lens epithelial cell proliferation, migration, and matrix formation: An in vitro study |
title_short | The effects of rapamycin on lens epithelial cell proliferation, migration, and matrix formation: An in vitro study |
title_sort | effects of rapamycin on lens epithelial cell proliferation, migration, and matrix formation: an in vitro study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2927374/ https://www.ncbi.nlm.nih.gov/pubmed/20806034 |
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