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Identification a novel MYOC gene mutation in a Chinese family with juvenile-onset open angle glaucoma

PURPOSE: To describe the clinical and genetic findings in one Chinese family with juvenile-onset open angle glaucoma (JOAG). METHODS: One family was examined clinically and a follow-up took place 5 years later. After informed consent was obtained, genomic DNA was extracted from the venous blood of a...

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Autores principales: Zhao, Xin, Yang, Chaoshan, Tong, Yi, Zhang, Xiaohui, Xu, Liang, Li, Yang
Formato: Texto
Lenguaje:English
Publicado: Molecular Vision 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2927375/
https://www.ncbi.nlm.nih.gov/pubmed/20806035
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author Zhao, Xin
Yang, Chaoshan
Tong, Yi
Zhang, Xiaohui
Xu, Liang
Li, Yang
author_facet Zhao, Xin
Yang, Chaoshan
Tong, Yi
Zhang, Xiaohui
Xu, Liang
Li, Yang
author_sort Zhao, Xin
collection PubMed
description PURPOSE: To describe the clinical and genetic findings in one Chinese family with juvenile-onset open angle glaucoma (JOAG). METHODS: One family was examined clinically and a follow-up took place 5 years later. After informed consent was obtained, genomic DNA was extracted from the venous blood of all participants. Linkage analysis was performed with three microsatellite markers around the MYOC gene (D1S196, D1S2815, and D1S218) in the family. Mutation screening of all coding exons of MYOC was performed by direct sequencing of PCR-amplified DNA fragments and restriction fragment length polymorphism (RFLP) analysis. Bioinformatics analysis by the Garnier-Osguthorpe-Robson (GOR) method predicted the effects of variants detected on secondary structures of the MYOC protein. RESULTS: Clinical examination and pedigree analysis revealed a three- generation family with seven members diagnosed with JOAG, three with ocular hypertension, and five normal individuals. Through genotyping, the pedigree showed a linkage to the MYOC on chromosome 1q24–25. Mutation screening of MYOC in this family revealed an A→T transition at position 1348 (p. N450Y) of the cDNA sequence. This missense mutation co-segregated with the disease phenotype of the family, but was not found in 100 normal controls. Secondary structure prediction of the p.N450Y by the GOR method revealed the replacement of a coil with a β sheet at the amino acid 447. CONCLUSIONS: Early onset JOAG, with incomplete penetrance, is consistent with a novel mutation in MYOC. The finding provides pre-symptomatic molecular diagnosis for the members of this family and is useful for further genetic consultation.
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spelling pubmed-29273752010-08-30 Identification a novel MYOC gene mutation in a Chinese family with juvenile-onset open angle glaucoma Zhao, Xin Yang, Chaoshan Tong, Yi Zhang, Xiaohui Xu, Liang Li, Yang Mol Vis Research Article PURPOSE: To describe the clinical and genetic findings in one Chinese family with juvenile-onset open angle glaucoma (JOAG). METHODS: One family was examined clinically and a follow-up took place 5 years later. After informed consent was obtained, genomic DNA was extracted from the venous blood of all participants. Linkage analysis was performed with three microsatellite markers around the MYOC gene (D1S196, D1S2815, and D1S218) in the family. Mutation screening of all coding exons of MYOC was performed by direct sequencing of PCR-amplified DNA fragments and restriction fragment length polymorphism (RFLP) analysis. Bioinformatics analysis by the Garnier-Osguthorpe-Robson (GOR) method predicted the effects of variants detected on secondary structures of the MYOC protein. RESULTS: Clinical examination and pedigree analysis revealed a three- generation family with seven members diagnosed with JOAG, three with ocular hypertension, and five normal individuals. Through genotyping, the pedigree showed a linkage to the MYOC on chromosome 1q24–25. Mutation screening of MYOC in this family revealed an A→T transition at position 1348 (p. N450Y) of the cDNA sequence. This missense mutation co-segregated with the disease phenotype of the family, but was not found in 100 normal controls. Secondary structure prediction of the p.N450Y by the GOR method revealed the replacement of a coil with a β sheet at the amino acid 447. CONCLUSIONS: Early onset JOAG, with incomplete penetrance, is consistent with a novel mutation in MYOC. The finding provides pre-symptomatic molecular diagnosis for the members of this family and is useful for further genetic consultation. Molecular Vision 2010-08-25 /pmc/articles/PMC2927375/ /pubmed/20806035 Text en Copyright © 2010 Molecular Vision. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhao, Xin
Yang, Chaoshan
Tong, Yi
Zhang, Xiaohui
Xu, Liang
Li, Yang
Identification a novel MYOC gene mutation in a Chinese family with juvenile-onset open angle glaucoma
title Identification a novel MYOC gene mutation in a Chinese family with juvenile-onset open angle glaucoma
title_full Identification a novel MYOC gene mutation in a Chinese family with juvenile-onset open angle glaucoma
title_fullStr Identification a novel MYOC gene mutation in a Chinese family with juvenile-onset open angle glaucoma
title_full_unstemmed Identification a novel MYOC gene mutation in a Chinese family with juvenile-onset open angle glaucoma
title_short Identification a novel MYOC gene mutation in a Chinese family with juvenile-onset open angle glaucoma
title_sort identification a novel myoc gene mutation in a chinese family with juvenile-onset open angle glaucoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2927375/
https://www.ncbi.nlm.nih.gov/pubmed/20806035
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