Cargando…
Dcas Supports Cell Polarization and Cell-Cell Adhesion Complexes in Development
Mammalian Cas proteins regulate cell migration, division and survival, and are often deregulated in cancer. However, the presence of four paralogous Cas family members in mammals (BCAR1/p130Cas, EFS/Sin1, NEDD9/HEF1/Cas-L, and CASS4/HEPL) has limited their analysis in development. We deleted the sin...
Autores principales: | , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2927436/ https://www.ncbi.nlm.nih.gov/pubmed/20808771 http://dx.doi.org/10.1371/journal.pone.0012369 |
_version_ | 1782185752285675520 |
---|---|
author | Tikhmyanova, Nadezhda Tulin, Alexei V. Roegiers, Fabrice Golemis, Erica A. |
author_facet | Tikhmyanova, Nadezhda Tulin, Alexei V. Roegiers, Fabrice Golemis, Erica A. |
author_sort | Tikhmyanova, Nadezhda |
collection | PubMed |
description | Mammalian Cas proteins regulate cell migration, division and survival, and are often deregulated in cancer. However, the presence of four paralogous Cas family members in mammals (BCAR1/p130Cas, EFS/Sin1, NEDD9/HEF1/Cas-L, and CASS4/HEPL) has limited their analysis in development. We deleted the single Drosophila Cas gene, Dcas, to probe the developmental function of Dcas. Loss of Dcas had limited effect on embryonal development. However, we found that Dcas is an important modulator of the severity of the developmental phenotypes of mutations affecting integrins (If and mew) and their downstream effectors Fak56D or Src42A. Strikingly, embryonic lethal Fak56D-Dcas double mutant embryos had extensive cell polarity defects, including mislocalization and reduced expression of E-cadherin. Further genetic analysis established that loss of Dcas modified the embryonal lethal phenotypes of embryos with mutations in E-cadherin (Shg) or its signaling partners p120- and β-catenin (Arm). These results support an important role for Cas proteins in cell-cell adhesion signaling in development. |
format | Text |
id | pubmed-2927436 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-29274362010-08-31 Dcas Supports Cell Polarization and Cell-Cell Adhesion Complexes in Development Tikhmyanova, Nadezhda Tulin, Alexei V. Roegiers, Fabrice Golemis, Erica A. PLoS One Research Article Mammalian Cas proteins regulate cell migration, division and survival, and are often deregulated in cancer. However, the presence of four paralogous Cas family members in mammals (BCAR1/p130Cas, EFS/Sin1, NEDD9/HEF1/Cas-L, and CASS4/HEPL) has limited their analysis in development. We deleted the single Drosophila Cas gene, Dcas, to probe the developmental function of Dcas. Loss of Dcas had limited effect on embryonal development. However, we found that Dcas is an important modulator of the severity of the developmental phenotypes of mutations affecting integrins (If and mew) and their downstream effectors Fak56D or Src42A. Strikingly, embryonic lethal Fak56D-Dcas double mutant embryos had extensive cell polarity defects, including mislocalization and reduced expression of E-cadherin. Further genetic analysis established that loss of Dcas modified the embryonal lethal phenotypes of embryos with mutations in E-cadherin (Shg) or its signaling partners p120- and β-catenin (Arm). These results support an important role for Cas proteins in cell-cell adhesion signaling in development. Public Library of Science 2010-08-24 /pmc/articles/PMC2927436/ /pubmed/20808771 http://dx.doi.org/10.1371/journal.pone.0012369 Text en Tikhmyanova et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Tikhmyanova, Nadezhda Tulin, Alexei V. Roegiers, Fabrice Golemis, Erica A. Dcas Supports Cell Polarization and Cell-Cell Adhesion Complexes in Development |
title | Dcas Supports Cell Polarization and Cell-Cell Adhesion Complexes in Development |
title_full | Dcas Supports Cell Polarization and Cell-Cell Adhesion Complexes in Development |
title_fullStr | Dcas Supports Cell Polarization and Cell-Cell Adhesion Complexes in Development |
title_full_unstemmed | Dcas Supports Cell Polarization and Cell-Cell Adhesion Complexes in Development |
title_short | Dcas Supports Cell Polarization and Cell-Cell Adhesion Complexes in Development |
title_sort | dcas supports cell polarization and cell-cell adhesion complexes in development |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2927436/ https://www.ncbi.nlm.nih.gov/pubmed/20808771 http://dx.doi.org/10.1371/journal.pone.0012369 |
work_keys_str_mv | AT tikhmyanovanadezhda dcassupportscellpolarizationandcellcelladhesioncomplexesindevelopment AT tulinalexeiv dcassupportscellpolarizationandcellcelladhesioncomplexesindevelopment AT roegiersfabrice dcassupportscellpolarizationandcellcelladhesioncomplexesindevelopment AT golemisericaa dcassupportscellpolarizationandcellcelladhesioncomplexesindevelopment |