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Dcas Supports Cell Polarization and Cell-Cell Adhesion Complexes in Development

Mammalian Cas proteins regulate cell migration, division and survival, and are often deregulated in cancer. However, the presence of four paralogous Cas family members in mammals (BCAR1/p130Cas, EFS/Sin1, NEDD9/HEF1/Cas-L, and CASS4/HEPL) has limited their analysis in development. We deleted the sin...

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Autores principales: Tikhmyanova, Nadezhda, Tulin, Alexei V., Roegiers, Fabrice, Golemis, Erica A.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2927436/
https://www.ncbi.nlm.nih.gov/pubmed/20808771
http://dx.doi.org/10.1371/journal.pone.0012369
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author Tikhmyanova, Nadezhda
Tulin, Alexei V.
Roegiers, Fabrice
Golemis, Erica A.
author_facet Tikhmyanova, Nadezhda
Tulin, Alexei V.
Roegiers, Fabrice
Golemis, Erica A.
author_sort Tikhmyanova, Nadezhda
collection PubMed
description Mammalian Cas proteins regulate cell migration, division and survival, and are often deregulated in cancer. However, the presence of four paralogous Cas family members in mammals (BCAR1/p130Cas, EFS/Sin1, NEDD9/HEF1/Cas-L, and CASS4/HEPL) has limited their analysis in development. We deleted the single Drosophila Cas gene, Dcas, to probe the developmental function of Dcas. Loss of Dcas had limited effect on embryonal development. However, we found that Dcas is an important modulator of the severity of the developmental phenotypes of mutations affecting integrins (If and mew) and their downstream effectors Fak56D or Src42A. Strikingly, embryonic lethal Fak56D-Dcas double mutant embryos had extensive cell polarity defects, including mislocalization and reduced expression of E-cadherin. Further genetic analysis established that loss of Dcas modified the embryonal lethal phenotypes of embryos with mutations in E-cadherin (Shg) or its signaling partners p120- and β-catenin (Arm). These results support an important role for Cas proteins in cell-cell adhesion signaling in development.
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spelling pubmed-29274362010-08-31 Dcas Supports Cell Polarization and Cell-Cell Adhesion Complexes in Development Tikhmyanova, Nadezhda Tulin, Alexei V. Roegiers, Fabrice Golemis, Erica A. PLoS One Research Article Mammalian Cas proteins regulate cell migration, division and survival, and are often deregulated in cancer. However, the presence of four paralogous Cas family members in mammals (BCAR1/p130Cas, EFS/Sin1, NEDD9/HEF1/Cas-L, and CASS4/HEPL) has limited their analysis in development. We deleted the single Drosophila Cas gene, Dcas, to probe the developmental function of Dcas. Loss of Dcas had limited effect on embryonal development. However, we found that Dcas is an important modulator of the severity of the developmental phenotypes of mutations affecting integrins (If and mew) and their downstream effectors Fak56D or Src42A. Strikingly, embryonic lethal Fak56D-Dcas double mutant embryos had extensive cell polarity defects, including mislocalization and reduced expression of E-cadherin. Further genetic analysis established that loss of Dcas modified the embryonal lethal phenotypes of embryos with mutations in E-cadherin (Shg) or its signaling partners p120- and β-catenin (Arm). These results support an important role for Cas proteins in cell-cell adhesion signaling in development. Public Library of Science 2010-08-24 /pmc/articles/PMC2927436/ /pubmed/20808771 http://dx.doi.org/10.1371/journal.pone.0012369 Text en Tikhmyanova et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Tikhmyanova, Nadezhda
Tulin, Alexei V.
Roegiers, Fabrice
Golemis, Erica A.
Dcas Supports Cell Polarization and Cell-Cell Adhesion Complexes in Development
title Dcas Supports Cell Polarization and Cell-Cell Adhesion Complexes in Development
title_full Dcas Supports Cell Polarization and Cell-Cell Adhesion Complexes in Development
title_fullStr Dcas Supports Cell Polarization and Cell-Cell Adhesion Complexes in Development
title_full_unstemmed Dcas Supports Cell Polarization and Cell-Cell Adhesion Complexes in Development
title_short Dcas Supports Cell Polarization and Cell-Cell Adhesion Complexes in Development
title_sort dcas supports cell polarization and cell-cell adhesion complexes in development
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2927436/
https://www.ncbi.nlm.nih.gov/pubmed/20808771
http://dx.doi.org/10.1371/journal.pone.0012369
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