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Contribution of neutrophil elastase to the lysis of obliterative thrombi in the context of their platelet and fibrin content

Leukocytes invade newly formed thrombi through interactions with platelets and fibrin and later contribute to the removal of fibrin deposits mainly through the action of neutrophil elastase. The present study attempts to express in quantitative terms the impact of neutrophils on the lytic processes...

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Autores principales: Rábai, Gyöngyi, Szilágyi, Nóra, Sótonyi, Péter, Kovalszky, Ilona, Szabó, László, Machovich, Raymund, Kolev, Krasimir
Formato: Texto
Lenguaje:English
Publicado: Pergamon Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2927910/
https://www.ncbi.nlm.nih.gov/pubmed/20627279
http://dx.doi.org/10.1016/j.thromres.2010.05.007
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author Rábai, Gyöngyi
Szilágyi, Nóra
Sótonyi, Péter
Kovalszky, Ilona
Szabó, László
Machovich, Raymund
Kolev, Krasimir
author_facet Rábai, Gyöngyi
Szilágyi, Nóra
Sótonyi, Péter
Kovalszky, Ilona
Szabó, László
Machovich, Raymund
Kolev, Krasimir
author_sort Rábai, Gyöngyi
collection PubMed
description Leukocytes invade newly formed thrombi through interactions with platelets and fibrin and later contribute to the removal of fibrin deposits mainly through the action of neutrophil elastase. The present study attempts to express in quantitative terms the impact of neutrophils on the lytic processes in obliterative thrombi based on the local presence of elastase-specific fibrin degradation products (NE-FDP) in relation to the leukocyte, platelet and fibrin content of thrombi. Immunofluorescent detection of fibrin, NE-FDP and platelet antigens was performed in sections of thrombi from 28 patients subjected to thrombectomy in combination with DNA-staining for identification of nucleated cells. The digitalized fluorescent microscopic images were decomposed according to the color channel of each thrombus constituent. The integrated intensity values for all thrombus constituents were statistically evaluated with correlation, hierarchical agglomerative clustering , Hotelling's T(2) and F-statistics. Association between NE-FDP and leukocyte content of thrombi is evidenced by a significant Pearson correlation coefficient of 0.71 (p = 0.00002). Cluster analysis reveals two classes of thrombi according to NE-FDP, leukocyte and platelet content and also two according to NE-FDP, leukocyte and fibrin content. When NE-FDP, fibrin and platelet content is normalized to the leukocyte count in the same thrombus, clusters with platelet-related thrombolytic resistance (inversely related NE-FDP and platelet content) and advanced cell-dependent thrombolysis (inversely related NE-FDP and fibrin content) are identified. These distinct patterns of thrombus constituents are snapshots of characteristic stages in the cell-dependent thrombolysis, which indicate a clot-stabilizing role for platelets in this process similar to their impact on the plasmin-dependent lysis.
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spelling pubmed-29279102010-09-20 Contribution of neutrophil elastase to the lysis of obliterative thrombi in the context of their platelet and fibrin content Rábai, Gyöngyi Szilágyi, Nóra Sótonyi, Péter Kovalszky, Ilona Szabó, László Machovich, Raymund Kolev, Krasimir Thromb Res Regular Article Leukocytes invade newly formed thrombi through interactions with platelets and fibrin and later contribute to the removal of fibrin deposits mainly through the action of neutrophil elastase. The present study attempts to express in quantitative terms the impact of neutrophils on the lytic processes in obliterative thrombi based on the local presence of elastase-specific fibrin degradation products (NE-FDP) in relation to the leukocyte, platelet and fibrin content of thrombi. Immunofluorescent detection of fibrin, NE-FDP and platelet antigens was performed in sections of thrombi from 28 patients subjected to thrombectomy in combination with DNA-staining for identification of nucleated cells. The digitalized fluorescent microscopic images were decomposed according to the color channel of each thrombus constituent. The integrated intensity values for all thrombus constituents were statistically evaluated with correlation, hierarchical agglomerative clustering , Hotelling's T(2) and F-statistics. Association between NE-FDP and leukocyte content of thrombi is evidenced by a significant Pearson correlation coefficient of 0.71 (p = 0.00002). Cluster analysis reveals two classes of thrombi according to NE-FDP, leukocyte and platelet content and also two according to NE-FDP, leukocyte and fibrin content. When NE-FDP, fibrin and platelet content is normalized to the leukocyte count in the same thrombus, clusters with platelet-related thrombolytic resistance (inversely related NE-FDP and platelet content) and advanced cell-dependent thrombolysis (inversely related NE-FDP and fibrin content) are identified. These distinct patterns of thrombus constituents are snapshots of characteristic stages in the cell-dependent thrombolysis, which indicate a clot-stabilizing role for platelets in this process similar to their impact on the plasmin-dependent lysis. Pergamon Press 2010-08 /pmc/articles/PMC2927910/ /pubmed/20627279 http://dx.doi.org/10.1016/j.thromres.2010.05.007 Text en © 2010 Elsevier Ltd. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Regular Article
Rábai, Gyöngyi
Szilágyi, Nóra
Sótonyi, Péter
Kovalszky, Ilona
Szabó, László
Machovich, Raymund
Kolev, Krasimir
Contribution of neutrophil elastase to the lysis of obliterative thrombi in the context of their platelet and fibrin content
title Contribution of neutrophil elastase to the lysis of obliterative thrombi in the context of their platelet and fibrin content
title_full Contribution of neutrophil elastase to the lysis of obliterative thrombi in the context of their platelet and fibrin content
title_fullStr Contribution of neutrophil elastase to the lysis of obliterative thrombi in the context of their platelet and fibrin content
title_full_unstemmed Contribution of neutrophil elastase to the lysis of obliterative thrombi in the context of their platelet and fibrin content
title_short Contribution of neutrophil elastase to the lysis of obliterative thrombi in the context of their platelet and fibrin content
title_sort contribution of neutrophil elastase to the lysis of obliterative thrombi in the context of their platelet and fibrin content
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2927910/
https://www.ncbi.nlm.nih.gov/pubmed/20627279
http://dx.doi.org/10.1016/j.thromres.2010.05.007
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