Structure of hibernating ribosomes studied by cryoelectron tomography in vitro and in situ

Ribosomes arranged in pairs (100S) have been related with nutritional stress response and are believed to represent a “hibernation state.” Several proteins have been identified that are associated with 100S ribosomes but their spatial organization has hitherto not been characterized. We have used cr...

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Autores principales: Ortiz, Julio O., Brandt, Florian, Matias, Valério R.F., Sennels, Lau, Rappsilber, Juri, Scheres, Sjors H.W., Eibauer, Matthias, Hartl, F. Ulrich, Baumeister, Wolfgang
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2010
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2928015/
https://www.ncbi.nlm.nih.gov/pubmed/20733057
http://dx.doi.org/10.1083/jcb.201005007
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author Ortiz, Julio O.
Brandt, Florian
Matias, Valério R.F.
Sennels, Lau
Rappsilber, Juri
Scheres, Sjors H.W.
Eibauer, Matthias
Hartl, F. Ulrich
Baumeister, Wolfgang
author_facet Ortiz, Julio O.
Brandt, Florian
Matias, Valério R.F.
Sennels, Lau
Rappsilber, Juri
Scheres, Sjors H.W.
Eibauer, Matthias
Hartl, F. Ulrich
Baumeister, Wolfgang
author_sort Ortiz, Julio O.
collection PubMed
description Ribosomes arranged in pairs (100S) have been related with nutritional stress response and are believed to represent a “hibernation state.” Several proteins have been identified that are associated with 100S ribosomes but their spatial organization has hitherto not been characterized. We have used cryoelectron tomography to reveal the three-dimensional configuration of 100S ribosomes isolated from starved Escherichia coli cells and we have described their mode of interaction. In situ studies with intact E. coli cells allowed us to demonstrate that 100S ribosomes do exist in vivo and represent an easily reversible state of quiescence; they readily vanish when the growth medium is replenished.
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spelling pubmed-29280152011-02-23 Structure of hibernating ribosomes studied by cryoelectron tomography in vitro and in situ Ortiz, Julio O. Brandt, Florian Matias, Valério R.F. Sennels, Lau Rappsilber, Juri Scheres, Sjors H.W. Eibauer, Matthias Hartl, F. Ulrich Baumeister, Wolfgang J Cell Biol Research Articles Ribosomes arranged in pairs (100S) have been related with nutritional stress response and are believed to represent a “hibernation state.” Several proteins have been identified that are associated with 100S ribosomes but their spatial organization has hitherto not been characterized. We have used cryoelectron tomography to reveal the three-dimensional configuration of 100S ribosomes isolated from starved Escherichia coli cells and we have described their mode of interaction. In situ studies with intact E. coli cells allowed us to demonstrate that 100S ribosomes do exist in vivo and represent an easily reversible state of quiescence; they readily vanish when the growth medium is replenished. The Rockefeller University Press 2010-08-23 /pmc/articles/PMC2928015/ /pubmed/20733057 http://dx.doi.org/10.1083/jcb.201005007 Text en © 2010 Ortiz et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Ortiz, Julio O.
Brandt, Florian
Matias, Valério R.F.
Sennels, Lau
Rappsilber, Juri
Scheres, Sjors H.W.
Eibauer, Matthias
Hartl, F. Ulrich
Baumeister, Wolfgang
Structure of hibernating ribosomes studied by cryoelectron tomography in vitro and in situ
title Structure of hibernating ribosomes studied by cryoelectron tomography in vitro and in situ
title_full Structure of hibernating ribosomes studied by cryoelectron tomography in vitro and in situ
title_fullStr Structure of hibernating ribosomes studied by cryoelectron tomography in vitro and in situ
title_full_unstemmed Structure of hibernating ribosomes studied by cryoelectron tomography in vitro and in situ
title_short Structure of hibernating ribosomes studied by cryoelectron tomography in vitro and in situ
title_sort structure of hibernating ribosomes studied by cryoelectron tomography in vitro and in situ
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2928015/
https://www.ncbi.nlm.nih.gov/pubmed/20733057
http://dx.doi.org/10.1083/jcb.201005007
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