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A Comprehensive Comparison of Transmembrane Domains Reveals Organelle-Specific Properties

The various membranes of eukaryotic cells differ in composition, but it is at present unclear if this results in differences in physical properties. The sequences of transmembrane domains (TMDs) of integral membrane proteins should reflect the physical properties of the bilayers in which they reside...

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Detalles Bibliográficos
Autores principales: Sharpe, Hayley J., Stevens, Tim J., Munro, Sean
Formato: Texto
Lenguaje:English
Publicado: Cell Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2928124/
https://www.ncbi.nlm.nih.gov/pubmed/20603021
http://dx.doi.org/10.1016/j.cell.2010.05.037
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author Sharpe, Hayley J.
Stevens, Tim J.
Munro, Sean
author_facet Sharpe, Hayley J.
Stevens, Tim J.
Munro, Sean
author_sort Sharpe, Hayley J.
collection PubMed
description The various membranes of eukaryotic cells differ in composition, but it is at present unclear if this results in differences in physical properties. The sequences of transmembrane domains (TMDs) of integral membrane proteins should reflect the physical properties of the bilayers in which they reside. We used large datasets from both fungi and vertebrates to perform a comprehensive comparison of the TMDs of proteins from different organelles. We find that TMDs are not generic but have organelle-specific properties with a dichotomy in TMD length between the early and late parts of the secretory pathway. In addition, TMDs from post-ER organelles show striking asymmetries in amino acid compositions across the bilayer that is linked to residue size and varies between organelles. The pervasive presence of organelle-specific features among the TMDs of a particular organelle has implications for TMD prediction, regulation of protein activity by location, and sorting of proteins and lipids in the secretory pathway.
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spelling pubmed-29281242010-09-03 A Comprehensive Comparison of Transmembrane Domains Reveals Organelle-Specific Properties Sharpe, Hayley J. Stevens, Tim J. Munro, Sean Cell Resource The various membranes of eukaryotic cells differ in composition, but it is at present unclear if this results in differences in physical properties. The sequences of transmembrane domains (TMDs) of integral membrane proteins should reflect the physical properties of the bilayers in which they reside. We used large datasets from both fungi and vertebrates to perform a comprehensive comparison of the TMDs of proteins from different organelles. We find that TMDs are not generic but have organelle-specific properties with a dichotomy in TMD length between the early and late parts of the secretory pathway. In addition, TMDs from post-ER organelles show striking asymmetries in amino acid compositions across the bilayer that is linked to residue size and varies between organelles. The pervasive presence of organelle-specific features among the TMDs of a particular organelle has implications for TMD prediction, regulation of protein activity by location, and sorting of proteins and lipids in the secretory pathway. Cell Press 2010-07-09 /pmc/articles/PMC2928124/ /pubmed/20603021 http://dx.doi.org/10.1016/j.cell.2010.05.037 Text en © 2010 ELL & Excerpta Medica. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Resource
Sharpe, Hayley J.
Stevens, Tim J.
Munro, Sean
A Comprehensive Comparison of Transmembrane Domains Reveals Organelle-Specific Properties
title A Comprehensive Comparison of Transmembrane Domains Reveals Organelle-Specific Properties
title_full A Comprehensive Comparison of Transmembrane Domains Reveals Organelle-Specific Properties
title_fullStr A Comprehensive Comparison of Transmembrane Domains Reveals Organelle-Specific Properties
title_full_unstemmed A Comprehensive Comparison of Transmembrane Domains Reveals Organelle-Specific Properties
title_short A Comprehensive Comparison of Transmembrane Domains Reveals Organelle-Specific Properties
title_sort comprehensive comparison of transmembrane domains reveals organelle-specific properties
topic Resource
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2928124/
https://www.ncbi.nlm.nih.gov/pubmed/20603021
http://dx.doi.org/10.1016/j.cell.2010.05.037
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