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Prognostic significance of lymphangiogenesis in pharyngolaryngeal carcinoma patients

BACKGROUND: Lymphatic vessel spread is considered a major route for head and neck squamous cell carcinoma metastasis. Formation of new lymphatic vessels could facilitate the process, raising the malignant potential of these tumours. Recent identification of lymphatic markers allows the study of the...

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Autores principales: Garcia-Carracedo, Darío, Rodrigo, Juan Pablo, Astudillo, Aurora, Nieto, Carlos Suarez, Gonzalez, Maria Victoria
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2928795/
https://www.ncbi.nlm.nih.gov/pubmed/20698954
http://dx.doi.org/10.1186/1471-2407-10-416
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author Garcia-Carracedo, Darío
Rodrigo, Juan Pablo
Astudillo, Aurora
Nieto, Carlos Suarez
Gonzalez, Maria Victoria
author_facet Garcia-Carracedo, Darío
Rodrigo, Juan Pablo
Astudillo, Aurora
Nieto, Carlos Suarez
Gonzalez, Maria Victoria
author_sort Garcia-Carracedo, Darío
collection PubMed
description BACKGROUND: Lymphatic vessel spread is considered a major route for head and neck squamous cell carcinoma metastasis. Formation of new lymphatic vessels could facilitate the process, raising the malignant potential of these tumours. Recent identification of lymphatic markers allows the study of the lymphangiogenesis phenomenon. We searched for molecular events involved in the lymphangiogenic process that could have prognostic value in laryngeal/pharyngeal carcinoma patients. METHODS: 104 paraffin-embedded pharyngeal/laryngeal tumour samples were studied. Immunohistochemical analysis of podoplanin and double immunofluorescence analysis of Ki-67 and D2-40 were performed. Lymph vessel density (inside the tumour mass, at its periphery or considered as a whole) and the presence of tumour emboli inside lymphatics were recorded. The proliferative state of endothelial lymphatic cells was evaluated. RESULTS: Lymphatic vessels were detected inside the tumour mass (75%) and in the surrounding tissue (80%); some of them in a proliferative state. Tumour emboli were detected in a high proportion of the cases (45%). Lymphatic vessel density was higher in the pharyngeal cases (p = 0.0029), in greater size (p = 0.039), more advanced stage primary tumours (p = 0.006) and in carcinomas of patients with affected nodes (p = 0.019). The presence of tumour emboli and a high global vessel density were indicators of poor prognosis (recorded as death from tumour) in the laryngeal group (p = 0.015 and p = 0.027, respectively), but notably not in the pharyngeal one. Interestingly, high global vessel density showed a negative prognostic value among pathologically staged N0 laryngeal carcinomas (p = 0.03). CONCLUSIONS: The lymphangiogenic process correlated with aggressive tumour features (pN category, tumour size, tumour stage), but might play different roles in tumours arising from different anatomic sites. Our results suggest that detection of tumour emboli and assessment of global vessel density using the D2-40 antibody, may be useful in the clinical practice, as predictors of reduced survival among pN0 laryngeal carcinoma patients.
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spelling pubmed-29287952010-08-27 Prognostic significance of lymphangiogenesis in pharyngolaryngeal carcinoma patients Garcia-Carracedo, Darío Rodrigo, Juan Pablo Astudillo, Aurora Nieto, Carlos Suarez Gonzalez, Maria Victoria BMC Cancer Research Article BACKGROUND: Lymphatic vessel spread is considered a major route for head and neck squamous cell carcinoma metastasis. Formation of new lymphatic vessels could facilitate the process, raising the malignant potential of these tumours. Recent identification of lymphatic markers allows the study of the lymphangiogenesis phenomenon. We searched for molecular events involved in the lymphangiogenic process that could have prognostic value in laryngeal/pharyngeal carcinoma patients. METHODS: 104 paraffin-embedded pharyngeal/laryngeal tumour samples were studied. Immunohistochemical analysis of podoplanin and double immunofluorescence analysis of Ki-67 and D2-40 were performed. Lymph vessel density (inside the tumour mass, at its periphery or considered as a whole) and the presence of tumour emboli inside lymphatics were recorded. The proliferative state of endothelial lymphatic cells was evaluated. RESULTS: Lymphatic vessels were detected inside the tumour mass (75%) and in the surrounding tissue (80%); some of them in a proliferative state. Tumour emboli were detected in a high proportion of the cases (45%). Lymphatic vessel density was higher in the pharyngeal cases (p = 0.0029), in greater size (p = 0.039), more advanced stage primary tumours (p = 0.006) and in carcinomas of patients with affected nodes (p = 0.019). The presence of tumour emboli and a high global vessel density were indicators of poor prognosis (recorded as death from tumour) in the laryngeal group (p = 0.015 and p = 0.027, respectively), but notably not in the pharyngeal one. Interestingly, high global vessel density showed a negative prognostic value among pathologically staged N0 laryngeal carcinomas (p = 0.03). CONCLUSIONS: The lymphangiogenic process correlated with aggressive tumour features (pN category, tumour size, tumour stage), but might play different roles in tumours arising from different anatomic sites. Our results suggest that detection of tumour emboli and assessment of global vessel density using the D2-40 antibody, may be useful in the clinical practice, as predictors of reduced survival among pN0 laryngeal carcinoma patients. BioMed Central 2010-08-10 /pmc/articles/PMC2928795/ /pubmed/20698954 http://dx.doi.org/10.1186/1471-2407-10-416 Text en Copyright ©2010 Garcia-Carracedo et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Garcia-Carracedo, Darío
Rodrigo, Juan Pablo
Astudillo, Aurora
Nieto, Carlos Suarez
Gonzalez, Maria Victoria
Prognostic significance of lymphangiogenesis in pharyngolaryngeal carcinoma patients
title Prognostic significance of lymphangiogenesis in pharyngolaryngeal carcinoma patients
title_full Prognostic significance of lymphangiogenesis in pharyngolaryngeal carcinoma patients
title_fullStr Prognostic significance of lymphangiogenesis in pharyngolaryngeal carcinoma patients
title_full_unstemmed Prognostic significance of lymphangiogenesis in pharyngolaryngeal carcinoma patients
title_short Prognostic significance of lymphangiogenesis in pharyngolaryngeal carcinoma patients
title_sort prognostic significance of lymphangiogenesis in pharyngolaryngeal carcinoma patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2928795/
https://www.ncbi.nlm.nih.gov/pubmed/20698954
http://dx.doi.org/10.1186/1471-2407-10-416
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