Cargando…
Production of erythriod cells from human embryonic stem cells by fetal liver cell extract treatment
BACKGROUND: We recently developed a new method to induce human stem cells (hESCs) differentiation into hematopoietic progenitors by cell extract treatment. Here, we report an efficient strategy to generate erythroid progenitors from hESCs using cell extract from human fetal liver tissue (hFLT) with...
Autores principales: | , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2929223/ https://www.ncbi.nlm.nih.gov/pubmed/20696076 http://dx.doi.org/10.1186/1471-213X-10-85 |
_version_ | 1782185913475923968 |
---|---|
author | Liu, Yu-xiao Yue, Wen Ji, Lei Nan, Xue Pei, Xue-tao |
author_facet | Liu, Yu-xiao Yue, Wen Ji, Lei Nan, Xue Pei, Xue-tao |
author_sort | Liu, Yu-xiao |
collection | PubMed |
description | BACKGROUND: We recently developed a new method to induce human stem cells (hESCs) differentiation into hematopoietic progenitors by cell extract treatment. Here, we report an efficient strategy to generate erythroid progenitors from hESCs using cell extract from human fetal liver tissue (hFLT) with cytokines. Human embryoid bodies (hEBs) obtained of human H1 hESCs were treated with cell extract from hFLT and co-cultured with human fetal liver stromal cells (hFLSCs) feeder to induce hematopoietic cells. After the 11 days of treatment, hEBs were isolated and transplanted into liquid medium with hematopoietic cytokines for erythroid differentiation. Characteristics of the erythroid cells were analyzed by flow cytometry, Wright-Giemsa staining, real-time RT-PCR and related functional assays. RESULTS: The erythroid cells produced from hEBs could differentiate into enucleated cells and expressed globins in a time-dependent manner. They expressed not only embryonic globins but also the adult-globin with the maturation of the erythroid cells. In addition, our data showed that the hEBs-derived erythroid cells were able to act as oxygen carriers, indicating that hESCs could generate functional mature erythroid cells. CONCLUSION: Cell extract exposure with the addition of cytokines resulted in robust erythroid -like differentiation of hEBs and these hEBs-derived erythroid cells possessed functions similar to mature red blood cells. |
format | Text |
id | pubmed-2929223 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-29292232010-08-28 Production of erythriod cells from human embryonic stem cells by fetal liver cell extract treatment Liu, Yu-xiao Yue, Wen Ji, Lei Nan, Xue Pei, Xue-tao BMC Dev Biol Methodology Article BACKGROUND: We recently developed a new method to induce human stem cells (hESCs) differentiation into hematopoietic progenitors by cell extract treatment. Here, we report an efficient strategy to generate erythroid progenitors from hESCs using cell extract from human fetal liver tissue (hFLT) with cytokines. Human embryoid bodies (hEBs) obtained of human H1 hESCs were treated with cell extract from hFLT and co-cultured with human fetal liver stromal cells (hFLSCs) feeder to induce hematopoietic cells. After the 11 days of treatment, hEBs were isolated and transplanted into liquid medium with hematopoietic cytokines for erythroid differentiation. Characteristics of the erythroid cells were analyzed by flow cytometry, Wright-Giemsa staining, real-time RT-PCR and related functional assays. RESULTS: The erythroid cells produced from hEBs could differentiate into enucleated cells and expressed globins in a time-dependent manner. They expressed not only embryonic globins but also the adult-globin with the maturation of the erythroid cells. In addition, our data showed that the hEBs-derived erythroid cells were able to act as oxygen carriers, indicating that hESCs could generate functional mature erythroid cells. CONCLUSION: Cell extract exposure with the addition of cytokines resulted in robust erythroid -like differentiation of hEBs and these hEBs-derived erythroid cells possessed functions similar to mature red blood cells. BioMed Central 2010-08-10 /pmc/articles/PMC2929223/ /pubmed/20696076 http://dx.doi.org/10.1186/1471-213X-10-85 Text en Copyright ©2010 Liu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Methodology Article Liu, Yu-xiao Yue, Wen Ji, Lei Nan, Xue Pei, Xue-tao Production of erythriod cells from human embryonic stem cells by fetal liver cell extract treatment |
title | Production of erythriod cells from human embryonic stem cells by fetal liver cell extract treatment |
title_full | Production of erythriod cells from human embryonic stem cells by fetal liver cell extract treatment |
title_fullStr | Production of erythriod cells from human embryonic stem cells by fetal liver cell extract treatment |
title_full_unstemmed | Production of erythriod cells from human embryonic stem cells by fetal liver cell extract treatment |
title_short | Production of erythriod cells from human embryonic stem cells by fetal liver cell extract treatment |
title_sort | production of erythriod cells from human embryonic stem cells by fetal liver cell extract treatment |
topic | Methodology Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2929223/ https://www.ncbi.nlm.nih.gov/pubmed/20696076 http://dx.doi.org/10.1186/1471-213X-10-85 |
work_keys_str_mv | AT liuyuxiao productionoferythriodcellsfromhumanembryonicstemcellsbyfetallivercellextracttreatment AT yuewen productionoferythriodcellsfromhumanembryonicstemcellsbyfetallivercellextracttreatment AT jilei productionoferythriodcellsfromhumanembryonicstemcellsbyfetallivercellextracttreatment AT nanxue productionoferythriodcellsfromhumanembryonicstemcellsbyfetallivercellextracttreatment AT peixuetao productionoferythriodcellsfromhumanembryonicstemcellsbyfetallivercellextracttreatment |