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Hypermethylation of SOX2 Promoter in Endometrial Carcinogenesis

This paper aimed at investigating the expression and methylation profiles of SOX2, a gene coding for the stem cell-related transcription factor SOX2, in endometrial carcinomas. By methylation-specific polymerase chain reaction (MS-PCR), the methylation status of SOX2 promoter region in 72 endometria...

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Autores principales: Wong, Oscar Gee-Wan, Huo, Zhen, Siu, Michelle Kwan-Yee, Zhang, HuiJuan, Jiang, LiLi, Wong, Ester Shuk-Ying, Cheung, Annie Nga-Yin
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2929617/
https://www.ncbi.nlm.nih.gov/pubmed/20814443
http://dx.doi.org/10.1155/2010/682504
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author Wong, Oscar Gee-Wan
Huo, Zhen
Siu, Michelle Kwan-Yee
Zhang, HuiJuan
Jiang, LiLi
Wong, Ester Shuk-Ying
Cheung, Annie Nga-Yin
author_facet Wong, Oscar Gee-Wan
Huo, Zhen
Siu, Michelle Kwan-Yee
Zhang, HuiJuan
Jiang, LiLi
Wong, Ester Shuk-Ying
Cheung, Annie Nga-Yin
author_sort Wong, Oscar Gee-Wan
collection PubMed
description This paper aimed at investigating the expression and methylation profiles of SOX2, a gene coding for the stem cell-related transcription factor SOX2, in endometrial carcinomas. By methylation-specific polymerase chain reaction (MS-PCR), the methylation status of SOX2 promoter region in 72 endometrial carcinomas and 12 normal endometrial samples was examined. Methylated allele was found in 37.5% (27/72) of endometrial carcinomas but only in 8.3% (1/12) of normal endometrial, significantly more frequent in cancers (P = .0472). SOX2 mRNA level was significantly reduced in endometrial carcinoma compared with nonneoplastic endometrium (P = .045). A significant correlation between SOX2 mRNA expression and hypermethylation of SOX2 was found (P = .024). Hypermethylation of SOX2 tended to be more frequently found in type II serous or clear cell adenocarcinoma. SOX2 methylation was also significantly correlated with shorter survival of patients (P = .046). In conclusion, epigenetic mechanisms may play a crucial role on the transcriptional regulation of SOX2 and loss of SOX2 expression may be related to endometrial carcinogenesis.
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spelling pubmed-29296172010-09-02 Hypermethylation of SOX2 Promoter in Endometrial Carcinogenesis Wong, Oscar Gee-Wan Huo, Zhen Siu, Michelle Kwan-Yee Zhang, HuiJuan Jiang, LiLi Wong, Ester Shuk-Ying Cheung, Annie Nga-Yin Obstet Gynecol Int Research Article This paper aimed at investigating the expression and methylation profiles of SOX2, a gene coding for the stem cell-related transcription factor SOX2, in endometrial carcinomas. By methylation-specific polymerase chain reaction (MS-PCR), the methylation status of SOX2 promoter region in 72 endometrial carcinomas and 12 normal endometrial samples was examined. Methylated allele was found in 37.5% (27/72) of endometrial carcinomas but only in 8.3% (1/12) of normal endometrial, significantly more frequent in cancers (P = .0472). SOX2 mRNA level was significantly reduced in endometrial carcinoma compared with nonneoplastic endometrium (P = .045). A significant correlation between SOX2 mRNA expression and hypermethylation of SOX2 was found (P = .024). Hypermethylation of SOX2 tended to be more frequently found in type II serous or clear cell adenocarcinoma. SOX2 methylation was also significantly correlated with shorter survival of patients (P = .046). In conclusion, epigenetic mechanisms may play a crucial role on the transcriptional regulation of SOX2 and loss of SOX2 expression may be related to endometrial carcinogenesis. Hindawi Publishing Corporation 2010 2010-08-09 /pmc/articles/PMC2929617/ /pubmed/20814443 http://dx.doi.org/10.1155/2010/682504 Text en Copyright © 2010 Oscar Gee-Wan Wong et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wong, Oscar Gee-Wan
Huo, Zhen
Siu, Michelle Kwan-Yee
Zhang, HuiJuan
Jiang, LiLi
Wong, Ester Shuk-Ying
Cheung, Annie Nga-Yin
Hypermethylation of SOX2 Promoter in Endometrial Carcinogenesis
title Hypermethylation of SOX2 Promoter in Endometrial Carcinogenesis
title_full Hypermethylation of SOX2 Promoter in Endometrial Carcinogenesis
title_fullStr Hypermethylation of SOX2 Promoter in Endometrial Carcinogenesis
title_full_unstemmed Hypermethylation of SOX2 Promoter in Endometrial Carcinogenesis
title_short Hypermethylation of SOX2 Promoter in Endometrial Carcinogenesis
title_sort hypermethylation of sox2 promoter in endometrial carcinogenesis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2929617/
https://www.ncbi.nlm.nih.gov/pubmed/20814443
http://dx.doi.org/10.1155/2010/682504
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