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Development and Validation of a RP-HPLC Method for Determination of Cyclosporine in Capsule

A simple, specific and accurate reverse phase high performance liquid chromatographic method was developed for the determination of cyclosporine in capsule dosage form. XTerra C18 column was used as stationary phase with mobile phase acetonitrile in combination with 0.1% trifluoro acetic acid buffer...

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Detalles Bibliográficos
Autores principales: Aziz, F., Gupta, A., Khan, M. F.
Formato: Texto
Lenguaje:English
Publicado: Medknow Publications 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2929790/
https://www.ncbi.nlm.nih.gov/pubmed/20838535
http://dx.doi.org/10.4103/0250-474X.65030
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author Aziz, F.
Gupta, A.
Khan, M. F.
author_facet Aziz, F.
Gupta, A.
Khan, M. F.
author_sort Aziz, F.
collection PubMed
description A simple, specific and accurate reverse phase high performance liquid chromatographic method was developed for the determination of cyclosporine in capsule dosage form. XTerra C18 column was used as stationary phase with mobile phase acetonitrile in combination with 0.1% trifluoro acetic acid buffer and pH is adjusted to 1.4. Method was developed in an isocratic run of 20% trifluoro acetic acid with 80% acetonitrile for 10 min, at flow rate of 1 ml/min. Effluents were monitored at 210 nm. Retention time of cyclosporine was 3.855 min. The method was validated for specificity, linearity, accuracy, precision, limit of quantification, limit of detection, robustness and solution stability. Limit of quantification and limit of detection of cyclosporine was found to be 100 ng/ml and 200 ng/ml. Recovery was found to be in the range of 98.08-101.55%. The proposed method was successfully applied for the quantitative determination of cyclosporine in a capsule dosage form.
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spelling pubmed-29297902010-09-13 Development and Validation of a RP-HPLC Method for Determination of Cyclosporine in Capsule Aziz, F. Gupta, A. Khan, M. F. Indian J Pharm Sci Short Communication A simple, specific and accurate reverse phase high performance liquid chromatographic method was developed for the determination of cyclosporine in capsule dosage form. XTerra C18 column was used as stationary phase with mobile phase acetonitrile in combination with 0.1% trifluoro acetic acid buffer and pH is adjusted to 1.4. Method was developed in an isocratic run of 20% trifluoro acetic acid with 80% acetonitrile for 10 min, at flow rate of 1 ml/min. Effluents were monitored at 210 nm. Retention time of cyclosporine was 3.855 min. The method was validated for specificity, linearity, accuracy, precision, limit of quantification, limit of detection, robustness and solution stability. Limit of quantification and limit of detection of cyclosporine was found to be 100 ng/ml and 200 ng/ml. Recovery was found to be in the range of 98.08-101.55%. The proposed method was successfully applied for the quantitative determination of cyclosporine in a capsule dosage form. Medknow Publications 2010 /pmc/articles/PMC2929790/ /pubmed/20838535 http://dx.doi.org/10.4103/0250-474X.65030 Text en © Indian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Aziz, F.
Gupta, A.
Khan, M. F.
Development and Validation of a RP-HPLC Method for Determination of Cyclosporine in Capsule
title Development and Validation of a RP-HPLC Method for Determination of Cyclosporine in Capsule
title_full Development and Validation of a RP-HPLC Method for Determination of Cyclosporine in Capsule
title_fullStr Development and Validation of a RP-HPLC Method for Determination of Cyclosporine in Capsule
title_full_unstemmed Development and Validation of a RP-HPLC Method for Determination of Cyclosporine in Capsule
title_short Development and Validation of a RP-HPLC Method for Determination of Cyclosporine in Capsule
title_sort development and validation of a rp-hplc method for determination of cyclosporine in capsule
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2929790/
https://www.ncbi.nlm.nih.gov/pubmed/20838535
http://dx.doi.org/10.4103/0250-474X.65030
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