Cargando…

Requirement for distinct vesicle-associated membrane proteins in insulin- and AMP-activated protein kinase (AMPK)-induced translocation of GLUT4 and CD36 in cultured cardiomyocytes

AIMS/HYPOTHESIS: Upon stimulation of insulin signalling or contraction-induced AMP-activated protein kinase (AMPK) activation, the glucose transporter GLUT4 and the long-chain fatty acid (LCFA) transporter CD36 similarly translocate from intracellular compartments to the plasma membrane of cardiomyo...

Descripción completa

Detalles Bibliográficos
Autores principales: Schwenk, R. W., Dirkx, E., Coumans, W. A., Bonen, A., Klip, A., Glatz, J. F. C., Luiken, J. J. F. P.
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2931635/
https://www.ncbi.nlm.nih.gov/pubmed/20582536
http://dx.doi.org/10.1007/s00125-010-1832-7
_version_ 1782186060010225664
author Schwenk, R. W.
Dirkx, E.
Coumans, W. A.
Bonen, A.
Klip, A.
Glatz, J. F. C.
Luiken, J. J. F. P.
author_facet Schwenk, R. W.
Dirkx, E.
Coumans, W. A.
Bonen, A.
Klip, A.
Glatz, J. F. C.
Luiken, J. J. F. P.
author_sort Schwenk, R. W.
collection PubMed
description AIMS/HYPOTHESIS: Upon stimulation of insulin signalling or contraction-induced AMP-activated protein kinase (AMPK) activation, the glucose transporter GLUT4 and the long-chain fatty acid (LCFA) transporter CD36 similarly translocate from intracellular compartments to the plasma membrane of cardiomyocytes to increase uptake of glucose and LCFA, respectively. This similarity in regulation of GLUT4 traffic and CD36 traffic suggests that the same families of trafficking proteins, including vesicle-associated membrane proteins (VAMPs), are involved in both processes. While several VAMPs have been implicated in GLUT4 traffic, nothing is known about the putative function of VAMPs in CD36 traffic. Therefore, we compared the involvement of the myocardially produced VAMP isoforms in insulin- or contraction-induced GLUT4 and CD36 translocation. METHODS: Five VAMP isoforms were silenced in HL-1 cardiomyocytes. The cells were treated with insulin or the contraction-like AMPK activator oligomycin or were electrically stimulated to contract. Subsequently, GLUT4 and CD36 translocation as well as substrate uptake were measured. RESULTS: Three VAMPs were demonstrated to be necessary for both GLUT4 and CD36 translocation, either specifically in insulin-treated cells (VAMP2, VAMP5) or in oligomycin/contraction-treated cells (VAMP3). In addition, there are VAMPs specifically involved in either GLUT4 traffic (VAMP7 mediates basal GLUT4 retention) or CD36 traffic (VAMP4 mediates insulin- and oligomycin/contraction-induced CD36 translocation). CONCLUSIONS/INTERPRETATION: The involvement of distinct VAMP isoforms in both GLUT4 and CD36 translocation indicates that CD36 translocation, just like GLUT4 translocation, is a vesicle-mediated process dependent on soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex formation. The ability of other VAMPs to discriminate between GLUT4 and CD36 translocation allows the notion that myocardial substrate preference can be modulated by these VAMPs.
format Text
id pubmed-2931635
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher Springer-Verlag
record_format MEDLINE/PubMed
spelling pubmed-29316352010-09-10 Requirement for distinct vesicle-associated membrane proteins in insulin- and AMP-activated protein kinase (AMPK)-induced translocation of GLUT4 and CD36 in cultured cardiomyocytes Schwenk, R. W. Dirkx, E. Coumans, W. A. Bonen, A. Klip, A. Glatz, J. F. C. Luiken, J. J. F. P. Diabetologia Article AIMS/HYPOTHESIS: Upon stimulation of insulin signalling or contraction-induced AMP-activated protein kinase (AMPK) activation, the glucose transporter GLUT4 and the long-chain fatty acid (LCFA) transporter CD36 similarly translocate from intracellular compartments to the plasma membrane of cardiomyocytes to increase uptake of glucose and LCFA, respectively. This similarity in regulation of GLUT4 traffic and CD36 traffic suggests that the same families of trafficking proteins, including vesicle-associated membrane proteins (VAMPs), are involved in both processes. While several VAMPs have been implicated in GLUT4 traffic, nothing is known about the putative function of VAMPs in CD36 traffic. Therefore, we compared the involvement of the myocardially produced VAMP isoforms in insulin- or contraction-induced GLUT4 and CD36 translocation. METHODS: Five VAMP isoforms were silenced in HL-1 cardiomyocytes. The cells were treated with insulin or the contraction-like AMPK activator oligomycin or were electrically stimulated to contract. Subsequently, GLUT4 and CD36 translocation as well as substrate uptake were measured. RESULTS: Three VAMPs were demonstrated to be necessary for both GLUT4 and CD36 translocation, either specifically in insulin-treated cells (VAMP2, VAMP5) or in oligomycin/contraction-treated cells (VAMP3). In addition, there are VAMPs specifically involved in either GLUT4 traffic (VAMP7 mediates basal GLUT4 retention) or CD36 traffic (VAMP4 mediates insulin- and oligomycin/contraction-induced CD36 translocation). CONCLUSIONS/INTERPRETATION: The involvement of distinct VAMP isoforms in both GLUT4 and CD36 translocation indicates that CD36 translocation, just like GLUT4 translocation, is a vesicle-mediated process dependent on soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex formation. The ability of other VAMPs to discriminate between GLUT4 and CD36 translocation allows the notion that myocardial substrate preference can be modulated by these VAMPs. Springer-Verlag 2010-06-26 2010 /pmc/articles/PMC2931635/ /pubmed/20582536 http://dx.doi.org/10.1007/s00125-010-1832-7 Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Article
Schwenk, R. W.
Dirkx, E.
Coumans, W. A.
Bonen, A.
Klip, A.
Glatz, J. F. C.
Luiken, J. J. F. P.
Requirement for distinct vesicle-associated membrane proteins in insulin- and AMP-activated protein kinase (AMPK)-induced translocation of GLUT4 and CD36 in cultured cardiomyocytes
title Requirement for distinct vesicle-associated membrane proteins in insulin- and AMP-activated protein kinase (AMPK)-induced translocation of GLUT4 and CD36 in cultured cardiomyocytes
title_full Requirement for distinct vesicle-associated membrane proteins in insulin- and AMP-activated protein kinase (AMPK)-induced translocation of GLUT4 and CD36 in cultured cardiomyocytes
title_fullStr Requirement for distinct vesicle-associated membrane proteins in insulin- and AMP-activated protein kinase (AMPK)-induced translocation of GLUT4 and CD36 in cultured cardiomyocytes
title_full_unstemmed Requirement for distinct vesicle-associated membrane proteins in insulin- and AMP-activated protein kinase (AMPK)-induced translocation of GLUT4 and CD36 in cultured cardiomyocytes
title_short Requirement for distinct vesicle-associated membrane proteins in insulin- and AMP-activated protein kinase (AMPK)-induced translocation of GLUT4 and CD36 in cultured cardiomyocytes
title_sort requirement for distinct vesicle-associated membrane proteins in insulin- and amp-activated protein kinase (ampk)-induced translocation of glut4 and cd36 in cultured cardiomyocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2931635/
https://www.ncbi.nlm.nih.gov/pubmed/20582536
http://dx.doi.org/10.1007/s00125-010-1832-7
work_keys_str_mv AT schwenkrw requirementfordistinctvesicleassociatedmembraneproteinsininsulinandampactivatedproteinkinaseampkinducedtranslocationofglut4andcd36inculturedcardiomyocytes
AT dirkxe requirementfordistinctvesicleassociatedmembraneproteinsininsulinandampactivatedproteinkinaseampkinducedtranslocationofglut4andcd36inculturedcardiomyocytes
AT coumanswa requirementfordistinctvesicleassociatedmembraneproteinsininsulinandampactivatedproteinkinaseampkinducedtranslocationofglut4andcd36inculturedcardiomyocytes
AT bonena requirementfordistinctvesicleassociatedmembraneproteinsininsulinandampactivatedproteinkinaseampkinducedtranslocationofglut4andcd36inculturedcardiomyocytes
AT klipa requirementfordistinctvesicleassociatedmembraneproteinsininsulinandampactivatedproteinkinaseampkinducedtranslocationofglut4andcd36inculturedcardiomyocytes
AT glatzjfc requirementfordistinctvesicleassociatedmembraneproteinsininsulinandampactivatedproteinkinaseampkinducedtranslocationofglut4andcd36inculturedcardiomyocytes
AT luikenjjfp requirementfordistinctvesicleassociatedmembraneproteinsininsulinandampactivatedproteinkinaseampkinducedtranslocationofglut4andcd36inculturedcardiomyocytes