Cargando…
Immunoexpression of the relaxin receptor LGR7 in breast and uterine tissues of humans and primates
BACKGROUND: The receptor for the peptide hormone relaxin has recently been identified as the heptahelical G-protein coupled receptor, LGR7. In order to generate molecular tools with which to characterize both in vivo and in vitro expression of this receptor in human and primate tissues, specific mon...
Autores principales: | , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2003
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC293425/ https://www.ncbi.nlm.nih.gov/pubmed/14633277 http://dx.doi.org/10.1186/1477-7827-1-114 |
_version_ | 1782121077297643520 |
---|---|
author | Ivell, Richard Balvers, Marga Pohnke, Yvonne Telgmann, Ralph Bartsch, Olaf Milde-Langosch, Karin Bamberger, Ana-maria Einspanier, Almuth |
author_facet | Ivell, Richard Balvers, Marga Pohnke, Yvonne Telgmann, Ralph Bartsch, Olaf Milde-Langosch, Karin Bamberger, Ana-maria Einspanier, Almuth |
author_sort | Ivell, Richard |
collection | PubMed |
description | BACKGROUND: The receptor for the peptide hormone relaxin has recently been identified as the heptahelical G-protein coupled receptor, LGR7. In order to generate molecular tools with which to characterize both in vivo and in vitro expression of this receptor in human and primate tissues, specific monotypic antibodies have been generated and applied to a preliminary analysis of human and primate female reproductive tissues. METHODS: Three peptide sequences were identified from the proposed open reading frame of the cloned LGR7 receptor gene, representing both extracellular and intracellular domains. Two to three rabbits were immunized for each epitope, and the resulting sera subjected to a systematic validation using cultured cells transiently transfected with a receptor-expressing gene construct, or appropriate control constructs. RESULTS: Human and monkey (marmoset, macaque) endometrium showed consistent and specific immunostaining in the stromal cells close to glands. Staining appeared to be more intense in the luteal phase of the cycle. Weak immunostaining was also evident in the endometrial epithelial cells of the marmoset. A myoma in one patient exhibited strong immunostaining in the circumscribing connective tissue. Uterine expression was supported by RT-PCR results from cultured primary endometrial and myometrial cells. Human breast tissue (healthy and tumors) consistently indicated specific immunostaining in the interstitial connective (stromal) tissue within the glands, but not in epithelial or myoepithelial cells, except in some tumors, where a few epithelial and tumor cells also showed weak epitope expression. CONCLUSIONS: Using validated monotypic antibodies recognizing different epitopes of the LGR7 receptor, and from different immunized animals, and in different primate species, a consistent pattern of LGR7 expression was observed in the stromal (connective tissue) cells of the endometrium and breast, consistent also with the known physiology of the relaxin hormone. |
format | Text |
id | pubmed-293425 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2003 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-2934252003-12-16 Immunoexpression of the relaxin receptor LGR7 in breast and uterine tissues of humans and primates Ivell, Richard Balvers, Marga Pohnke, Yvonne Telgmann, Ralph Bartsch, Olaf Milde-Langosch, Karin Bamberger, Ana-maria Einspanier, Almuth Reprod Biol Endocrinol Research BACKGROUND: The receptor for the peptide hormone relaxin has recently been identified as the heptahelical G-protein coupled receptor, LGR7. In order to generate molecular tools with which to characterize both in vivo and in vitro expression of this receptor in human and primate tissues, specific monotypic antibodies have been generated and applied to a preliminary analysis of human and primate female reproductive tissues. METHODS: Three peptide sequences were identified from the proposed open reading frame of the cloned LGR7 receptor gene, representing both extracellular and intracellular domains. Two to three rabbits were immunized for each epitope, and the resulting sera subjected to a systematic validation using cultured cells transiently transfected with a receptor-expressing gene construct, or appropriate control constructs. RESULTS: Human and monkey (marmoset, macaque) endometrium showed consistent and specific immunostaining in the stromal cells close to glands. Staining appeared to be more intense in the luteal phase of the cycle. Weak immunostaining was also evident in the endometrial epithelial cells of the marmoset. A myoma in one patient exhibited strong immunostaining in the circumscribing connective tissue. Uterine expression was supported by RT-PCR results from cultured primary endometrial and myometrial cells. Human breast tissue (healthy and tumors) consistently indicated specific immunostaining in the interstitial connective (stromal) tissue within the glands, but not in epithelial or myoepithelial cells, except in some tumors, where a few epithelial and tumor cells also showed weak epitope expression. CONCLUSIONS: Using validated monotypic antibodies recognizing different epitopes of the LGR7 receptor, and from different immunized animals, and in different primate species, a consistent pattern of LGR7 expression was observed in the stromal (connective tissue) cells of the endometrium and breast, consistent also with the known physiology of the relaxin hormone. BioMed Central 2003-11-24 /pmc/articles/PMC293425/ /pubmed/14633277 http://dx.doi.org/10.1186/1477-7827-1-114 Text en Copyright © 2003 Ivell et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. |
spellingShingle | Research Ivell, Richard Balvers, Marga Pohnke, Yvonne Telgmann, Ralph Bartsch, Olaf Milde-Langosch, Karin Bamberger, Ana-maria Einspanier, Almuth Immunoexpression of the relaxin receptor LGR7 in breast and uterine tissues of humans and primates |
title | Immunoexpression of the relaxin receptor LGR7 in breast and uterine tissues of humans and primates |
title_full | Immunoexpression of the relaxin receptor LGR7 in breast and uterine tissues of humans and primates |
title_fullStr | Immunoexpression of the relaxin receptor LGR7 in breast and uterine tissues of humans and primates |
title_full_unstemmed | Immunoexpression of the relaxin receptor LGR7 in breast and uterine tissues of humans and primates |
title_short | Immunoexpression of the relaxin receptor LGR7 in breast and uterine tissues of humans and primates |
title_sort | immunoexpression of the relaxin receptor lgr7 in breast and uterine tissues of humans and primates |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC293425/ https://www.ncbi.nlm.nih.gov/pubmed/14633277 http://dx.doi.org/10.1186/1477-7827-1-114 |
work_keys_str_mv | AT ivellrichard immunoexpressionoftherelaxinreceptorlgr7inbreastanduterinetissuesofhumansandprimates AT balversmarga immunoexpressionoftherelaxinreceptorlgr7inbreastanduterinetissuesofhumansandprimates AT pohnkeyvonne immunoexpressionoftherelaxinreceptorlgr7inbreastanduterinetissuesofhumansandprimates AT telgmannralph immunoexpressionoftherelaxinreceptorlgr7inbreastanduterinetissuesofhumansandprimates AT bartscholaf immunoexpressionoftherelaxinreceptorlgr7inbreastanduterinetissuesofhumansandprimates AT mildelangoschkarin immunoexpressionoftherelaxinreceptorlgr7inbreastanduterinetissuesofhumansandprimates AT bambergeranamaria immunoexpressionoftherelaxinreceptorlgr7inbreastanduterinetissuesofhumansandprimates AT einspanieralmuth immunoexpressionoftherelaxinreceptorlgr7inbreastanduterinetissuesofhumansandprimates |