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In vitro Assembly of Cubic RNA-Based Scaffolds Designed in silico

The organization of biological materials into versatile three-dimensional assemblies could be used to build multifunctional therapeutic scaffolds for use in nanomedicine. Here we report a strategy to design three-dimensional nanoscale scaffolds that can be self-assembled from RNA with precise contro...

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Autores principales: Afonin, Kirill A, Bindewald, Eckart, Yaghoubian, Alan J., Voss, Neil, Jacovetty, Erica, Shapiro, Bruce A., Jaeger, Luc
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2934861/
https://www.ncbi.nlm.nih.gov/pubmed/20802494
http://dx.doi.org/10.1038/nnano.2010.160
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author Afonin, Kirill A
Bindewald, Eckart
Yaghoubian, Alan J.
Voss, Neil
Jacovetty, Erica
Shapiro, Bruce A.
Jaeger, Luc
author_facet Afonin, Kirill A
Bindewald, Eckart
Yaghoubian, Alan J.
Voss, Neil
Jacovetty, Erica
Shapiro, Bruce A.
Jaeger, Luc
author_sort Afonin, Kirill A
collection PubMed
description The organization of biological materials into versatile three-dimensional assemblies could be used to build multifunctional therapeutic scaffolds for use in nanomedicine. Here we report a strategy to design three-dimensional nanoscale scaffolds that can be self-assembled from RNA with precise control over their shape, size and composition. These cubic nanoscaffolds are only ~13 nm in diameter and are composed of short oligonucleotides making them amenable to chemical synthesis, point modifications and further functionalization. Nanocube assembly is verified by gel assays, dynamic light scattering and cryogenic electron microscopy. Formation of functional RNA nanocubes is also demonstrated by incorporation of a light-up fluorescent RNA aptamer that is optimally active only upon full RNA assembly. Moreover, we show the RNA nano-scaffolds can self-assemble in isothermal conditions (37°C) during in vitro transcription, which opens a route towards the construction of sensors, programmable packaging and cargo delivery systems for biomedical applications.
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spelling pubmed-29348612011-03-01 In vitro Assembly of Cubic RNA-Based Scaffolds Designed in silico Afonin, Kirill A Bindewald, Eckart Yaghoubian, Alan J. Voss, Neil Jacovetty, Erica Shapiro, Bruce A. Jaeger, Luc Nat Nanotechnol Article The organization of biological materials into versatile three-dimensional assemblies could be used to build multifunctional therapeutic scaffolds for use in nanomedicine. Here we report a strategy to design three-dimensional nanoscale scaffolds that can be self-assembled from RNA with precise control over their shape, size and composition. These cubic nanoscaffolds are only ~13 nm in diameter and are composed of short oligonucleotides making them amenable to chemical synthesis, point modifications and further functionalization. Nanocube assembly is verified by gel assays, dynamic light scattering and cryogenic electron microscopy. Formation of functional RNA nanocubes is also demonstrated by incorporation of a light-up fluorescent RNA aptamer that is optimally active only upon full RNA assembly. Moreover, we show the RNA nano-scaffolds can self-assemble in isothermal conditions (37°C) during in vitro transcription, which opens a route towards the construction of sensors, programmable packaging and cargo delivery systems for biomedical applications. 2010-08-29 2010-09 /pmc/articles/PMC2934861/ /pubmed/20802494 http://dx.doi.org/10.1038/nnano.2010.160 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Afonin, Kirill A
Bindewald, Eckart
Yaghoubian, Alan J.
Voss, Neil
Jacovetty, Erica
Shapiro, Bruce A.
Jaeger, Luc
In vitro Assembly of Cubic RNA-Based Scaffolds Designed in silico
title In vitro Assembly of Cubic RNA-Based Scaffolds Designed in silico
title_full In vitro Assembly of Cubic RNA-Based Scaffolds Designed in silico
title_fullStr In vitro Assembly of Cubic RNA-Based Scaffolds Designed in silico
title_full_unstemmed In vitro Assembly of Cubic RNA-Based Scaffolds Designed in silico
title_short In vitro Assembly of Cubic RNA-Based Scaffolds Designed in silico
title_sort in vitro assembly of cubic rna-based scaffolds designed in silico
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2934861/
https://www.ncbi.nlm.nih.gov/pubmed/20802494
http://dx.doi.org/10.1038/nnano.2010.160
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