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A Tracer Analysis Study on the Redistribution and Oxidization of Endogenous Carbon Monoxide in the Human Body

Past studies have suggested that some carbon monoxide (CO) moves from blood haemoglobin to tissue cells and that mitochondrial cytochrome c oxidase oxidizes CO to carbon dioxide (CO(2)). However, no study has demonstrated this redistribution and oxidization of CO under physiological conditions. The...

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Autores principales: Sawano, Makoto, Shimouchi, Akito
Formato: Texto
Lenguaje:English
Publicado: the Society for Free Radical Research Japan 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2935150/
https://www.ncbi.nlm.nih.gov/pubmed/20838565
http://dx.doi.org/10.3164/jcbn.10-22
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author Sawano, Makoto
Shimouchi, Akito
author_facet Sawano, Makoto
Shimouchi, Akito
author_sort Sawano, Makoto
collection PubMed
description Past studies have suggested that some carbon monoxide (CO) moves from blood haemoglobin to tissue cells and that mitochondrial cytochrome c oxidase oxidizes CO to carbon dioxide (CO(2)). However, no study has demonstrated this redistribution and oxidization of CO under physiological conditions. The objective of this study was to trace the redistribution and oxidization of CO in the human body by detecting (13)CO(2) production after the inhalation of (13)CO. In Experiment 1, we asked a healthy subject to inhale 50 ppm (13)CO gas. In Experiment 2, we circulated heparinized human blood in a cardio-pulmonary bypass circuit and supplied 50 ppm (13)CO gas to the oxygenator. We sequentially sampled exhaled and output gases and measured the (13)CO(2)/(12)CO(2) ratios. In Experiment 1, the exhaled (13)CO(2)/(12)CO(2) ratio increased significantly between 4 to 31 h of (13)CO inhalation. In Experiment 2, the output (13)CO(2)/(12)CO(2) ratio showed no significant increase within 36 h of (13)CO input. Experiment 1 demonstrated the oxidization of CO in the human body under physiological conditions. Experiment 2 confirmed that oxidization does not occur in the circulating blood and indicated the redistribution of CO from blood carboxyhaemoglobin to tissue cells.
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spelling pubmed-29351502010-09-13 A Tracer Analysis Study on the Redistribution and Oxidization of Endogenous Carbon Monoxide in the Human Body Sawano, Makoto Shimouchi, Akito J Clin Biochem Nutr Original Article Past studies have suggested that some carbon monoxide (CO) moves from blood haemoglobin to tissue cells and that mitochondrial cytochrome c oxidase oxidizes CO to carbon dioxide (CO(2)). However, no study has demonstrated this redistribution and oxidization of CO under physiological conditions. The objective of this study was to trace the redistribution and oxidization of CO in the human body by detecting (13)CO(2) production after the inhalation of (13)CO. In Experiment 1, we asked a healthy subject to inhale 50 ppm (13)CO gas. In Experiment 2, we circulated heparinized human blood in a cardio-pulmonary bypass circuit and supplied 50 ppm (13)CO gas to the oxygenator. We sequentially sampled exhaled and output gases and measured the (13)CO(2)/(12)CO(2) ratios. In Experiment 1, the exhaled (13)CO(2)/(12)CO(2) ratio increased significantly between 4 to 31 h of (13)CO inhalation. In Experiment 2, the output (13)CO(2)/(12)CO(2) ratio showed no significant increase within 36 h of (13)CO input. Experiment 1 demonstrated the oxidization of CO in the human body under physiological conditions. Experiment 2 confirmed that oxidization does not occur in the circulating blood and indicated the redistribution of CO from blood carboxyhaemoglobin to tissue cells. the Society for Free Radical Research Japan 2010-09 2010-06-17 /pmc/articles/PMC2935150/ /pubmed/20838565 http://dx.doi.org/10.3164/jcbn.10-22 Text en Copyright © 2010 JCBN This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Sawano, Makoto
Shimouchi, Akito
A Tracer Analysis Study on the Redistribution and Oxidization of Endogenous Carbon Monoxide in the Human Body
title A Tracer Analysis Study on the Redistribution and Oxidization of Endogenous Carbon Monoxide in the Human Body
title_full A Tracer Analysis Study on the Redistribution and Oxidization of Endogenous Carbon Monoxide in the Human Body
title_fullStr A Tracer Analysis Study on the Redistribution and Oxidization of Endogenous Carbon Monoxide in the Human Body
title_full_unstemmed A Tracer Analysis Study on the Redistribution and Oxidization of Endogenous Carbon Monoxide in the Human Body
title_short A Tracer Analysis Study on the Redistribution and Oxidization of Endogenous Carbon Monoxide in the Human Body
title_sort tracer analysis study on the redistribution and oxidization of endogenous carbon monoxide in the human body
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2935150/
https://www.ncbi.nlm.nih.gov/pubmed/20838565
http://dx.doi.org/10.3164/jcbn.10-22
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