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Carotenoid fluorescence in Dunaliella salina
Dunaliella salina is a halotolerant green alga that is well known for its carotenoid producing capacity. The produced carotenoids are mainly stored in lipid globules. For various research purposes, such as production and extraction kinetics, we would like to determine and/or localise the carotenoid...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2935544/ https://www.ncbi.nlm.nih.gov/pubmed/20835349 http://dx.doi.org/10.1007/s10811-010-9505-y |
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author | Kleinegris, Dorinde M. M. van Es, Marjon A. Janssen, Marcel Brandenburg, Willem A. Wijffels, René H. |
author_facet | Kleinegris, Dorinde M. M. van Es, Marjon A. Janssen, Marcel Brandenburg, Willem A. Wijffels, René H. |
author_sort | Kleinegris, Dorinde M. M. |
collection | PubMed |
description | Dunaliella salina is a halotolerant green alga that is well known for its carotenoid producing capacity. The produced carotenoids are mainly stored in lipid globules. For various research purposes, such as production and extraction kinetics, we would like to determine and/or localise the carotenoid globules in vivo. In this study, we show that the carotenoid-rich globules emit clear green fluorescence, which can be used in, for example, fluorescence microscopy (e.g. CLSM) to obtain pictures of the cells and their carotenoid content. |
format | Text |
id | pubmed-2935544 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-29355442010-09-10 Carotenoid fluorescence in Dunaliella salina Kleinegris, Dorinde M. M. van Es, Marjon A. Janssen, Marcel Brandenburg, Willem A. Wijffels, René H. J Appl Phycol Article Dunaliella salina is a halotolerant green alga that is well known for its carotenoid producing capacity. The produced carotenoids are mainly stored in lipid globules. For various research purposes, such as production and extraction kinetics, we would like to determine and/or localise the carotenoid globules in vivo. In this study, we show that the carotenoid-rich globules emit clear green fluorescence, which can be used in, for example, fluorescence microscopy (e.g. CLSM) to obtain pictures of the cells and their carotenoid content. Springer Netherlands 2010-02-17 2010 /pmc/articles/PMC2935544/ /pubmed/20835349 http://dx.doi.org/10.1007/s10811-010-9505-y Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Article Kleinegris, Dorinde M. M. van Es, Marjon A. Janssen, Marcel Brandenburg, Willem A. Wijffels, René H. Carotenoid fluorescence in Dunaliella salina |
title | Carotenoid fluorescence in Dunaliella salina |
title_full | Carotenoid fluorescence in Dunaliella salina |
title_fullStr | Carotenoid fluorescence in Dunaliella salina |
title_full_unstemmed | Carotenoid fluorescence in Dunaliella salina |
title_short | Carotenoid fluorescence in Dunaliella salina |
title_sort | carotenoid fluorescence in dunaliella salina |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2935544/ https://www.ncbi.nlm.nih.gov/pubmed/20835349 http://dx.doi.org/10.1007/s10811-010-9505-y |
work_keys_str_mv | AT kleinegrisdorindemm carotenoidfluorescenceindunaliellasalina AT vanesmarjona carotenoidfluorescenceindunaliellasalina AT janssenmarcel carotenoidfluorescenceindunaliellasalina AT brandenburgwillema carotenoidfluorescenceindunaliellasalina AT wijffelsreneh carotenoidfluorescenceindunaliellasalina |