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Carotenoid fluorescence in Dunaliella salina

Dunaliella salina is a halotolerant green alga that is well known for its carotenoid producing capacity. The produced carotenoids are mainly stored in lipid globules. For various research purposes, such as production and extraction kinetics, we would like to determine and/or localise the carotenoid...

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Detalles Bibliográficos
Autores principales: Kleinegris, Dorinde M. M., van Es, Marjon A., Janssen, Marcel, Brandenburg, Willem A., Wijffels, René H.
Formato: Texto
Lenguaje:English
Publicado: Springer Netherlands 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2935544/
https://www.ncbi.nlm.nih.gov/pubmed/20835349
http://dx.doi.org/10.1007/s10811-010-9505-y
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author Kleinegris, Dorinde M. M.
van Es, Marjon A.
Janssen, Marcel
Brandenburg, Willem A.
Wijffels, René H.
author_facet Kleinegris, Dorinde M. M.
van Es, Marjon A.
Janssen, Marcel
Brandenburg, Willem A.
Wijffels, René H.
author_sort Kleinegris, Dorinde M. M.
collection PubMed
description Dunaliella salina is a halotolerant green alga that is well known for its carotenoid producing capacity. The produced carotenoids are mainly stored in lipid globules. For various research purposes, such as production and extraction kinetics, we would like to determine and/or localise the carotenoid globules in vivo. In this study, we show that the carotenoid-rich globules emit clear green fluorescence, which can be used in, for example, fluorescence microscopy (e.g. CLSM) to obtain pictures of the cells and their carotenoid content.
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spelling pubmed-29355442010-09-10 Carotenoid fluorescence in Dunaliella salina Kleinegris, Dorinde M. M. van Es, Marjon A. Janssen, Marcel Brandenburg, Willem A. Wijffels, René H. J Appl Phycol Article Dunaliella salina is a halotolerant green alga that is well known for its carotenoid producing capacity. The produced carotenoids are mainly stored in lipid globules. For various research purposes, such as production and extraction kinetics, we would like to determine and/or localise the carotenoid globules in vivo. In this study, we show that the carotenoid-rich globules emit clear green fluorescence, which can be used in, for example, fluorescence microscopy (e.g. CLSM) to obtain pictures of the cells and their carotenoid content. Springer Netherlands 2010-02-17 2010 /pmc/articles/PMC2935544/ /pubmed/20835349 http://dx.doi.org/10.1007/s10811-010-9505-y Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Article
Kleinegris, Dorinde M. M.
van Es, Marjon A.
Janssen, Marcel
Brandenburg, Willem A.
Wijffels, René H.
Carotenoid fluorescence in Dunaliella salina
title Carotenoid fluorescence in Dunaliella salina
title_full Carotenoid fluorescence in Dunaliella salina
title_fullStr Carotenoid fluorescence in Dunaliella salina
title_full_unstemmed Carotenoid fluorescence in Dunaliella salina
title_short Carotenoid fluorescence in Dunaliella salina
title_sort carotenoid fluorescence in dunaliella salina
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2935544/
https://www.ncbi.nlm.nih.gov/pubmed/20835349
http://dx.doi.org/10.1007/s10811-010-9505-y
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AT janssenmarcel carotenoidfluorescenceindunaliellasalina
AT brandenburgwillema carotenoidfluorescenceindunaliellasalina
AT wijffelsreneh carotenoidfluorescenceindunaliellasalina