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A testis-specific regulator of complex and hybrid N-glycan synthesis
Database analyses identified 4933434I20Rik as a glycosyltransferase-like gene expressed mainly in testicular germ cells and regulated during spermatogenesis. Expression of a membrane-bound form of the protein resulted in a marked and specific reduction in N-acetylglucosaminyltransferase I (GlcNAcT-I...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2935569/ https://www.ncbi.nlm.nih.gov/pubmed/20805325 http://dx.doi.org/10.1083/jcb.201004102 |
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author | Huang, Hung-Hsiang Stanley, Pamela |
author_facet | Huang, Hung-Hsiang Stanley, Pamela |
author_sort | Huang, Hung-Hsiang |
collection | PubMed |
description | Database analyses identified 4933434I20Rik as a glycosyltransferase-like gene expressed mainly in testicular germ cells and regulated during spermatogenesis. Expression of a membrane-bound form of the protein resulted in a marked and specific reduction in N-acetylglucosaminyltransferase I (GlcNAcT-I) activity and complex and hybrid N-glycan synthesis. Thus, the novel activity was termed GlcNAcT-I inhibitory protein (GnT1IP). Membrane-bound GnT1IP localizes to the ER, the ER-Golgi intermediate compartment (ERGIC), and the cis-Golgi. Coexpression of membrane-anchored GnT1IP with GlcNAcT-I causes association of the two proteins, inactivation of GlcNAcT-I, and mislocalization of GlcNAcT-I from the medial-Golgi to earlier compartments. Therefore, GnT1IP is a regulator of GlcNAcT-I and complex and hybrid N-glycan production. Importantly, the formation of high mannose N-glycans resulting from inhibition of GlcNAcT-I by GnT1IP markedly increases the adhesion of CHO cells to TM4 Sertoli cells. Testicular germ cells might use GnT1IP to induce the expression of high mannose N-glycans on glycoproteins, thereby facilitating Sertoli–germ cell attachment at a particular stage of spermatogenesis. |
format | Text |
id | pubmed-2935569 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-29355692011-03-06 A testis-specific regulator of complex and hybrid N-glycan synthesis Huang, Hung-Hsiang Stanley, Pamela J Cell Biol Research Articles Database analyses identified 4933434I20Rik as a glycosyltransferase-like gene expressed mainly in testicular germ cells and regulated during spermatogenesis. Expression of a membrane-bound form of the protein resulted in a marked and specific reduction in N-acetylglucosaminyltransferase I (GlcNAcT-I) activity and complex and hybrid N-glycan synthesis. Thus, the novel activity was termed GlcNAcT-I inhibitory protein (GnT1IP). Membrane-bound GnT1IP localizes to the ER, the ER-Golgi intermediate compartment (ERGIC), and the cis-Golgi. Coexpression of membrane-anchored GnT1IP with GlcNAcT-I causes association of the two proteins, inactivation of GlcNAcT-I, and mislocalization of GlcNAcT-I from the medial-Golgi to earlier compartments. Therefore, GnT1IP is a regulator of GlcNAcT-I and complex and hybrid N-glycan production. Importantly, the formation of high mannose N-glycans resulting from inhibition of GlcNAcT-I by GnT1IP markedly increases the adhesion of CHO cells to TM4 Sertoli cells. Testicular germ cells might use GnT1IP to induce the expression of high mannose N-glycans on glycoproteins, thereby facilitating Sertoli–germ cell attachment at a particular stage of spermatogenesis. The Rockefeller University Press 2010-09-06 /pmc/articles/PMC2935569/ /pubmed/20805325 http://dx.doi.org/10.1083/jcb.201004102 Text en © 2010 Huang and Stanley https://creativecommons.org/licenses/by-nc-sa/3.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ (https://creativecommons.org/licenses/by-nc-sa/3.0/) ). |
spellingShingle | Research Articles Huang, Hung-Hsiang Stanley, Pamela A testis-specific regulator of complex and hybrid N-glycan synthesis |
title | A testis-specific regulator of complex and hybrid N-glycan synthesis |
title_full | A testis-specific regulator of complex and hybrid N-glycan synthesis |
title_fullStr | A testis-specific regulator of complex and hybrid N-glycan synthesis |
title_full_unstemmed | A testis-specific regulator of complex and hybrid N-glycan synthesis |
title_short | A testis-specific regulator of complex and hybrid N-glycan synthesis |
title_sort | testis-specific regulator of complex and hybrid n-glycan synthesis |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2935569/ https://www.ncbi.nlm.nih.gov/pubmed/20805325 http://dx.doi.org/10.1083/jcb.201004102 |
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