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A testis-specific regulator of complex and hybrid N-glycan synthesis

Database analyses identified 4933434I20Rik as a glycosyltransferase-like gene expressed mainly in testicular germ cells and regulated during spermatogenesis. Expression of a membrane-bound form of the protein resulted in a marked and specific reduction in N-acetylglucosaminyltransferase I (GlcNAcT-I...

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Detalles Bibliográficos
Autores principales: Huang, Hung-Hsiang, Stanley, Pamela
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2935569/
https://www.ncbi.nlm.nih.gov/pubmed/20805325
http://dx.doi.org/10.1083/jcb.201004102
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author Huang, Hung-Hsiang
Stanley, Pamela
author_facet Huang, Hung-Hsiang
Stanley, Pamela
author_sort Huang, Hung-Hsiang
collection PubMed
description Database analyses identified 4933434I20Rik as a glycosyltransferase-like gene expressed mainly in testicular germ cells and regulated during spermatogenesis. Expression of a membrane-bound form of the protein resulted in a marked and specific reduction in N-acetylglucosaminyltransferase I (GlcNAcT-I) activity and complex and hybrid N-glycan synthesis. Thus, the novel activity was termed GlcNAcT-I inhibitory protein (GnT1IP). Membrane-bound GnT1IP localizes to the ER, the ER-Golgi intermediate compartment (ERGIC), and the cis-Golgi. Coexpression of membrane-anchored GnT1IP with GlcNAcT-I causes association of the two proteins, inactivation of GlcNAcT-I, and mislocalization of GlcNAcT-I from the medial-Golgi to earlier compartments. Therefore, GnT1IP is a regulator of GlcNAcT-I and complex and hybrid N-glycan production. Importantly, the formation of high mannose N-glycans resulting from inhibition of GlcNAcT-I by GnT1IP markedly increases the adhesion of CHO cells to TM4 Sertoli cells. Testicular germ cells might use GnT1IP to induce the expression of high mannose N-glycans on glycoproteins, thereby facilitating Sertoli–germ cell attachment at a particular stage of spermatogenesis.
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spelling pubmed-29355692011-03-06 A testis-specific regulator of complex and hybrid N-glycan synthesis Huang, Hung-Hsiang Stanley, Pamela J Cell Biol Research Articles Database analyses identified 4933434I20Rik as a glycosyltransferase-like gene expressed mainly in testicular germ cells and regulated during spermatogenesis. Expression of a membrane-bound form of the protein resulted in a marked and specific reduction in N-acetylglucosaminyltransferase I (GlcNAcT-I) activity and complex and hybrid N-glycan synthesis. Thus, the novel activity was termed GlcNAcT-I inhibitory protein (GnT1IP). Membrane-bound GnT1IP localizes to the ER, the ER-Golgi intermediate compartment (ERGIC), and the cis-Golgi. Coexpression of membrane-anchored GnT1IP with GlcNAcT-I causes association of the two proteins, inactivation of GlcNAcT-I, and mislocalization of GlcNAcT-I from the medial-Golgi to earlier compartments. Therefore, GnT1IP is a regulator of GlcNAcT-I and complex and hybrid N-glycan production. Importantly, the formation of high mannose N-glycans resulting from inhibition of GlcNAcT-I by GnT1IP markedly increases the adhesion of CHO cells to TM4 Sertoli cells. Testicular germ cells might use GnT1IP to induce the expression of high mannose N-glycans on glycoproteins, thereby facilitating Sertoli–germ cell attachment at a particular stage of spermatogenesis. The Rockefeller University Press 2010-09-06 /pmc/articles/PMC2935569/ /pubmed/20805325 http://dx.doi.org/10.1083/jcb.201004102 Text en © 2010 Huang and Stanley https://creativecommons.org/licenses/by-nc-sa/3.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ (https://creativecommons.org/licenses/by-nc-sa/3.0/) ).
spellingShingle Research Articles
Huang, Hung-Hsiang
Stanley, Pamela
A testis-specific regulator of complex and hybrid N-glycan synthesis
title A testis-specific regulator of complex and hybrid N-glycan synthesis
title_full A testis-specific regulator of complex and hybrid N-glycan synthesis
title_fullStr A testis-specific regulator of complex and hybrid N-glycan synthesis
title_full_unstemmed A testis-specific regulator of complex and hybrid N-glycan synthesis
title_short A testis-specific regulator of complex and hybrid N-glycan synthesis
title_sort testis-specific regulator of complex and hybrid n-glycan synthesis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2935569/
https://www.ncbi.nlm.nih.gov/pubmed/20805325
http://dx.doi.org/10.1083/jcb.201004102
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