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Comparative in Silico Analyses of Cpeb1–4 with Functional Predictions
BACKGROUND: Cytoplasmic polyadenylation element binding proteins (Cpebs) are a family of proteins that bind to defined groups of mRNAs and regulate their translation. While Cpebs were originally identified as important features of oocyte maturation, recent interest is due to their prospective roles...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Libertas Academica
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2935813/ https://www.ncbi.nlm.nih.gov/pubmed/20838664 |
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author | Wang, Xiang-Ping Cooper, Nigel G.F. |
author_facet | Wang, Xiang-Ping Cooper, Nigel G.F. |
author_sort | Wang, Xiang-Ping |
collection | PubMed |
description | BACKGROUND: Cytoplasmic polyadenylation element binding proteins (Cpebs) are a family of proteins that bind to defined groups of mRNAs and regulate their translation. While Cpebs were originally identified as important features of oocyte maturation, recent interest is due to their prospective roles in neural system plasticity. RESULTS: In this study we made use of bioinformatic tools and methods including NCBI Blast, UCSC Blat, and Invitrogen Vector NTI to comprehensively analyze all known isoforms of four mouse Cpeb paralogs extracted from the national UniGene, UniProt, and NCBI protein databases. We identified multiple alternative splicing variants for each Cpeb. Regions of commonality and distinctiveness were evident when comparing Cpeb2, 3, and 4. In addition, we performed cross-ortholog comparisons among multiple species. The exon patterns were generally conserved across vertebrates. Mouse and human isoforms were compared in greater detail as they are the most represented in the current databases. The homologous and distinct regions are strictly conserved in mouse Cpeb and human CPEB proteins. Novel variants were proposed based on cross-ortholog comparisons and validated using biological methods. The functions of the alternatively spliced regions were predicted using the Eukaryotic Linear Motif resource. CONCLUSIONS: Together, the large number of transcripts and proteins indicate the presence of a hitherto unappreciated complexity in the regulation and functions of Cpebs. The evolutionary retention of variable regions as described here is most likely an indication of their functional significance. |
format | Text |
id | pubmed-2935813 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Libertas Academica |
record_format | MEDLINE/PubMed |
spelling | pubmed-29358132010-09-13 Comparative in Silico Analyses of Cpeb1–4 with Functional Predictions Wang, Xiang-Ping Cooper, Nigel G.F. Bioinform Biol Insights Original Research BACKGROUND: Cytoplasmic polyadenylation element binding proteins (Cpebs) are a family of proteins that bind to defined groups of mRNAs and regulate their translation. While Cpebs were originally identified as important features of oocyte maturation, recent interest is due to their prospective roles in neural system plasticity. RESULTS: In this study we made use of bioinformatic tools and methods including NCBI Blast, UCSC Blat, and Invitrogen Vector NTI to comprehensively analyze all known isoforms of four mouse Cpeb paralogs extracted from the national UniGene, UniProt, and NCBI protein databases. We identified multiple alternative splicing variants for each Cpeb. Regions of commonality and distinctiveness were evident when comparing Cpeb2, 3, and 4. In addition, we performed cross-ortholog comparisons among multiple species. The exon patterns were generally conserved across vertebrates. Mouse and human isoforms were compared in greater detail as they are the most represented in the current databases. The homologous and distinct regions are strictly conserved in mouse Cpeb and human CPEB proteins. Novel variants were proposed based on cross-ortholog comparisons and validated using biological methods. The functions of the alternatively spliced regions were predicted using the Eukaryotic Linear Motif resource. CONCLUSIONS: Together, the large number of transcripts and proteins indicate the presence of a hitherto unappreciated complexity in the regulation and functions of Cpebs. The evolutionary retention of variable regions as described here is most likely an indication of their functional significance. Libertas Academica 2010-11-22 /pmc/articles/PMC2935813/ /pubmed/20838664 Text en © 2010 the author(s), publisher and licensee Libertas Academica Ltd. This is an open access article. Unrestricted non-commercial use is permitted provided the original work is properly cited. |
spellingShingle | Original Research Wang, Xiang-Ping Cooper, Nigel G.F. Comparative in Silico Analyses of Cpeb1–4 with Functional Predictions |
title | Comparative in Silico Analyses of Cpeb1–4 with Functional Predictions |
title_full | Comparative in Silico Analyses of Cpeb1–4 with Functional Predictions |
title_fullStr | Comparative in Silico Analyses of Cpeb1–4 with Functional Predictions |
title_full_unstemmed | Comparative in Silico Analyses of Cpeb1–4 with Functional Predictions |
title_short | Comparative in Silico Analyses of Cpeb1–4 with Functional Predictions |
title_sort | comparative in silico analyses of cpeb1–4 with functional predictions |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2935813/ https://www.ncbi.nlm.nih.gov/pubmed/20838664 |
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