Modules for C-terminal epitope tagging of Tetrahymena genes

Although epitope tagging has been widely used for analyzing protein function in many organisms, there are few genetic tools for epitope tagging in Tetrahymena. In this study, we describe several C-terminal epitope tagging modules that can be used to express tagged proteins in Tetrahymena cells by bo...

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Detalles Bibliográficos
Autores principales: Kataoka, Kensuke, Schoeberl, Ursula E., Mochizuki, Kazufumi
Formato: Texto
Lenguaje:English
Publicado: Elsevier Biomedical 2010
Materias:
Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2935961/
https://www.ncbi.nlm.nih.gov/pubmed/20624430
http://dx.doi.org/10.1016/j.mimet.2010.07.009
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author Kataoka, Kensuke
Schoeberl, Ursula E.
Mochizuki, Kazufumi
author_facet Kataoka, Kensuke
Schoeberl, Ursula E.
Mochizuki, Kazufumi
author_sort Kataoka, Kensuke
collection PubMed
description Although epitope tagging has been widely used for analyzing protein function in many organisms, there are few genetic tools for epitope tagging in Tetrahymena. In this study, we describe several C-terminal epitope tagging modules that can be used to express tagged proteins in Tetrahymena cells by both plasmid- and PCR-based strategies.
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spelling pubmed-29359612010-10-13 Modules for C-terminal epitope tagging of Tetrahymena genes Kataoka, Kensuke Schoeberl, Ursula E. Mochizuki, Kazufumi J Microbiol Methods Note Although epitope tagging has been widely used for analyzing protein function in many organisms, there are few genetic tools for epitope tagging in Tetrahymena. In this study, we describe several C-terminal epitope tagging modules that can be used to express tagged proteins in Tetrahymena cells by both plasmid- and PCR-based strategies. Elsevier Biomedical 2010-09 /pmc/articles/PMC2935961/ /pubmed/20624430 http://dx.doi.org/10.1016/j.mimet.2010.07.009 Text en © 2010 Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/3.0/ Open Access under CC BY-NC-ND 3.0 (https://creativecommons.org/licenses/by-nc-nd/3.0/) license
spellingShingle Note
Kataoka, Kensuke
Schoeberl, Ursula E.
Mochizuki, Kazufumi
Modules for C-terminal epitope tagging of Tetrahymena genes
title Modules for C-terminal epitope tagging of Tetrahymena genes
title_full Modules for C-terminal epitope tagging of Tetrahymena genes
title_fullStr Modules for C-terminal epitope tagging of Tetrahymena genes
title_full_unstemmed Modules for C-terminal epitope tagging of Tetrahymena genes
title_short Modules for C-terminal epitope tagging of Tetrahymena genes
title_sort modules for c-terminal epitope tagging of tetrahymena genes
topic Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2935961/
https://www.ncbi.nlm.nih.gov/pubmed/20624430
http://dx.doi.org/10.1016/j.mimet.2010.07.009
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