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Female sex hormones mediate the allergic lung reaction by regulating the release of inflammatory mediators and the expression of lung E-selectin in rats
BACKGROUND: Fluctuations of estradiol and progesterone levels caused by the menstrual cycle worsen asthma symptoms. Conflicting data are reported in literature regarding pro and anti-inflammatory properties of estradiol and progesterone. METHODS: Female Wistar rats were ovalbumin (OVA) sensitized 1...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2936382/ https://www.ncbi.nlm.nih.gov/pubmed/20735828 http://dx.doi.org/10.1186/1465-9921-11-115 |
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author | de Oliveira, Ana Paula Ligeiro Peron, Jean Pierre Schatzmann Damazo, Amilcar Sabino dos Santos Franco, Adriana Lino Domingos, Helori Vanni Oliani, Sonia Maria Oliveira-Filho, Ricardo Martins Vargaftig, Bernardo Boris Tavares-de-Lima, Wothan |
author_facet | de Oliveira, Ana Paula Ligeiro Peron, Jean Pierre Schatzmann Damazo, Amilcar Sabino dos Santos Franco, Adriana Lino Domingos, Helori Vanni Oliani, Sonia Maria Oliveira-Filho, Ricardo Martins Vargaftig, Bernardo Boris Tavares-de-Lima, Wothan |
author_sort | de Oliveira, Ana Paula Ligeiro |
collection | PubMed |
description | BACKGROUND: Fluctuations of estradiol and progesterone levels caused by the menstrual cycle worsen asthma symptoms. Conflicting data are reported in literature regarding pro and anti-inflammatory properties of estradiol and progesterone. METHODS: Female Wistar rats were ovalbumin (OVA) sensitized 1 day after resection of the ovaries (OVx). Control group consisted of sensitized-rats with intact ovaries (Sham-OVx). Allergic challenge was performed by aerosol (OVA 1%, 15 min) two weeks later. Twenty four hours after challenge, BAL, bone marrow and total blood cells were counted. Lung tissues were used as explants, for expontaneous cytokine secretion in vitro or for immunostaining of E-selectin. RESULTS: We observed an exacerbated cell recruitment into the lungs of OVx rats, reduced blood leukocytes counting and increased the number of bone marrow cells. Estradiol-treated OVx allergic rats reduced, and those treated with progesterone increased, respectively, the number of cells in the BAL and bone marrow. Lungs of OVx allergic rats significantly increased the E-selectin expression, an effect prevented by estradiol but not by progesterone treatment. Systemically, estradiol treatment increased the number of peripheral blood leukocytes in OVx allergic rats when compared to non treated-OVx allergic rats. Cultured-BAL cells of OVx allergic rats released elevated amounts of LTB(4 )and nitrites while bone marrow cells increased the release of TNF-α and nitrites. Estradiol treatment of OVx allergic rats was associated with a decreased release of TNF-α, IL-10, LTB(4 )and nitrites by bone marrow cells incubates. In contrast, estradiol caused an increase in IL-10 and NO release by cultured-BAL cells. Progesterone significantly increased TNF- α by cultured BAL cells and bone marrow cells. CONCLUSIONS: Data presented here suggest that upon hormonal oscillations the immune sensitization might trigger an allergic lung inflammation whose phenotype is under control of estradiol. Our data could contribute to the understanding of the protective role of estradiol in some cases of asthma symptoms in fertile ans post-menopausal women clinically observed. |
format | Text |
id | pubmed-2936382 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-29363822010-09-10 Female sex hormones mediate the allergic lung reaction by regulating the release of inflammatory mediators and the expression of lung E-selectin in rats de Oliveira, Ana Paula Ligeiro Peron, Jean Pierre Schatzmann Damazo, Amilcar Sabino dos Santos Franco, Adriana Lino Domingos, Helori Vanni Oliani, Sonia Maria Oliveira-Filho, Ricardo Martins Vargaftig, Bernardo Boris Tavares-de-Lima, Wothan Respir Res Research BACKGROUND: Fluctuations of estradiol and progesterone levels caused by the menstrual cycle worsen asthma symptoms. Conflicting data are reported in literature regarding pro and anti-inflammatory properties of estradiol and progesterone. METHODS: Female Wistar rats were ovalbumin (OVA) sensitized 1 day after resection of the ovaries (OVx). Control group consisted of sensitized-rats with intact ovaries (Sham-OVx). Allergic challenge was performed by aerosol (OVA 1%, 15 min) two weeks later. Twenty four hours after challenge, BAL, bone marrow and total blood cells were counted. Lung tissues were used as explants, for expontaneous cytokine secretion in vitro or for immunostaining of E-selectin. RESULTS: We observed an exacerbated cell recruitment into the lungs of OVx rats, reduced blood leukocytes counting and increased the number of bone marrow cells. Estradiol-treated OVx allergic rats reduced, and those treated with progesterone increased, respectively, the number of cells in the BAL and bone marrow. Lungs of OVx allergic rats significantly increased the E-selectin expression, an effect prevented by estradiol but not by progesterone treatment. Systemically, estradiol treatment increased the number of peripheral blood leukocytes in OVx allergic rats when compared to non treated-OVx allergic rats. Cultured-BAL cells of OVx allergic rats released elevated amounts of LTB(4 )and nitrites while bone marrow cells increased the release of TNF-α and nitrites. Estradiol treatment of OVx allergic rats was associated with a decreased release of TNF-α, IL-10, LTB(4 )and nitrites by bone marrow cells incubates. In contrast, estradiol caused an increase in IL-10 and NO release by cultured-BAL cells. Progesterone significantly increased TNF- α by cultured BAL cells and bone marrow cells. CONCLUSIONS: Data presented here suggest that upon hormonal oscillations the immune sensitization might trigger an allergic lung inflammation whose phenotype is under control of estradiol. Our data could contribute to the understanding of the protective role of estradiol in some cases of asthma symptoms in fertile ans post-menopausal women clinically observed. BioMed Central 2010 2010-08-24 /pmc/articles/PMC2936382/ /pubmed/20735828 http://dx.doi.org/10.1186/1465-9921-11-115 Text en Copyright ©2010 de Oliveira et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research de Oliveira, Ana Paula Ligeiro Peron, Jean Pierre Schatzmann Damazo, Amilcar Sabino dos Santos Franco, Adriana Lino Domingos, Helori Vanni Oliani, Sonia Maria Oliveira-Filho, Ricardo Martins Vargaftig, Bernardo Boris Tavares-de-Lima, Wothan Female sex hormones mediate the allergic lung reaction by regulating the release of inflammatory mediators and the expression of lung E-selectin in rats |
title | Female sex hormones mediate the allergic lung reaction by regulating the release of inflammatory mediators and the expression of lung E-selectin in rats |
title_full | Female sex hormones mediate the allergic lung reaction by regulating the release of inflammatory mediators and the expression of lung E-selectin in rats |
title_fullStr | Female sex hormones mediate the allergic lung reaction by regulating the release of inflammatory mediators and the expression of lung E-selectin in rats |
title_full_unstemmed | Female sex hormones mediate the allergic lung reaction by regulating the release of inflammatory mediators and the expression of lung E-selectin in rats |
title_short | Female sex hormones mediate the allergic lung reaction by regulating the release of inflammatory mediators and the expression of lung E-selectin in rats |
title_sort | female sex hormones mediate the allergic lung reaction by regulating the release of inflammatory mediators and the expression of lung e-selectin in rats |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2936382/ https://www.ncbi.nlm.nih.gov/pubmed/20735828 http://dx.doi.org/10.1186/1465-9921-11-115 |
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