Cargando…

A Central Regulatory System Largely Controls Transcriptional Activation and Repression Responses to Phosphate Starvation in Arabidopsis

Plants respond to different stresses by inducing or repressing transcription of partially overlapping sets of genes. In Arabidopsis, the PHR1 transcription factor (TF) has an important role in the control of phosphate (Pi) starvation stress responses. Using transcriptomic analysis of Pi starvation i...

Descripción completa

Detalles Bibliográficos
Autores principales: Bustos, Regla, Castrillo, Gabriel, Linhares, Francisco, Puga, María Isabel, Rubio, Vicente, Pérez-Pérez, Julian, Solano, Roberto, Leyva, Antonio, Paz-Ares, Javier
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2936532/
https://www.ncbi.nlm.nih.gov/pubmed/20838596
http://dx.doi.org/10.1371/journal.pgen.1001102
_version_ 1782186503507542016
author Bustos, Regla
Castrillo, Gabriel
Linhares, Francisco
Puga, María Isabel
Rubio, Vicente
Pérez-Pérez, Julian
Solano, Roberto
Leyva, Antonio
Paz-Ares, Javier
author_facet Bustos, Regla
Castrillo, Gabriel
Linhares, Francisco
Puga, María Isabel
Rubio, Vicente
Pérez-Pérez, Julian
Solano, Roberto
Leyva, Antonio
Paz-Ares, Javier
author_sort Bustos, Regla
collection PubMed
description Plants respond to different stresses by inducing or repressing transcription of partially overlapping sets of genes. In Arabidopsis, the PHR1 transcription factor (TF) has an important role in the control of phosphate (Pi) starvation stress responses. Using transcriptomic analysis of Pi starvation in phr1, and phr1 phr1-like (phl1) mutants and in wild type plants, we show that PHR1 in conjunction with PHL1 controls most transcriptional activation and repression responses to phosphate starvation, regardless of the Pi starvation specificity of these responses. Induced genes are enriched in PHR1 binding sequences (P1BS) in their promoters, whereas repressed genes do not show such enrichment, suggesting that PHR1(-like) control of transcriptional repression responses is indirect. In agreement with this, transcriptomic analysis of a transgenic plant expressing PHR1 fused to the hormone ligand domain of the glucocorticoid receptor showed that PHR1 direct targets (i.e., displaying altered expression after GR:PHR1 activation by dexamethasone in the presence of cycloheximide) corresponded largely to Pi starvation-induced genes that are highly enriched in P1BS. A minimal promoter containing a multimerised P1BS recapitulates Pi starvation-specific responsiveness. Likewise, mutation of P1BS in the promoter of two Pi starvation-responsive genes impaired their responsiveness to Pi starvation, but not to other stress types. Phylogenetic footprinting confirmed the importance of P1BS and PHR1 in Pi starvation responsiveness and indicated that P1BS acts in concert with other cis motifs. All together, our data show that PHR1 and PHL1 are partially redundant TF acting as central integrators of Pi starvation responses, both specific and generic. In addition, they indicate that transcriptional repression responses are an integral part of adaptive responses to stress.
format Text
id pubmed-2936532
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-29365322010-09-13 A Central Regulatory System Largely Controls Transcriptional Activation and Repression Responses to Phosphate Starvation in Arabidopsis Bustos, Regla Castrillo, Gabriel Linhares, Francisco Puga, María Isabel Rubio, Vicente Pérez-Pérez, Julian Solano, Roberto Leyva, Antonio Paz-Ares, Javier PLoS Genet Research Article Plants respond to different stresses by inducing or repressing transcription of partially overlapping sets of genes. In Arabidopsis, the PHR1 transcription factor (TF) has an important role in the control of phosphate (Pi) starvation stress responses. Using transcriptomic analysis of Pi starvation in phr1, and phr1 phr1-like (phl1) mutants and in wild type plants, we show that PHR1 in conjunction with PHL1 controls most transcriptional activation and repression responses to phosphate starvation, regardless of the Pi starvation specificity of these responses. Induced genes are enriched in PHR1 binding sequences (P1BS) in their promoters, whereas repressed genes do not show such enrichment, suggesting that PHR1(-like) control of transcriptional repression responses is indirect. In agreement with this, transcriptomic analysis of a transgenic plant expressing PHR1 fused to the hormone ligand domain of the glucocorticoid receptor showed that PHR1 direct targets (i.e., displaying altered expression after GR:PHR1 activation by dexamethasone in the presence of cycloheximide) corresponded largely to Pi starvation-induced genes that are highly enriched in P1BS. A minimal promoter containing a multimerised P1BS recapitulates Pi starvation-specific responsiveness. Likewise, mutation of P1BS in the promoter of two Pi starvation-responsive genes impaired their responsiveness to Pi starvation, but not to other stress types. Phylogenetic footprinting confirmed the importance of P1BS and PHR1 in Pi starvation responsiveness and indicated that P1BS acts in concert with other cis motifs. All together, our data show that PHR1 and PHL1 are partially redundant TF acting as central integrators of Pi starvation responses, both specific and generic. In addition, they indicate that transcriptional repression responses are an integral part of adaptive responses to stress. Public Library of Science 2010-09-09 /pmc/articles/PMC2936532/ /pubmed/20838596 http://dx.doi.org/10.1371/journal.pgen.1001102 Text en Bustos et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Bustos, Regla
Castrillo, Gabriel
Linhares, Francisco
Puga, María Isabel
Rubio, Vicente
Pérez-Pérez, Julian
Solano, Roberto
Leyva, Antonio
Paz-Ares, Javier
A Central Regulatory System Largely Controls Transcriptional Activation and Repression Responses to Phosphate Starvation in Arabidopsis
title A Central Regulatory System Largely Controls Transcriptional Activation and Repression Responses to Phosphate Starvation in Arabidopsis
title_full A Central Regulatory System Largely Controls Transcriptional Activation and Repression Responses to Phosphate Starvation in Arabidopsis
title_fullStr A Central Regulatory System Largely Controls Transcriptional Activation and Repression Responses to Phosphate Starvation in Arabidopsis
title_full_unstemmed A Central Regulatory System Largely Controls Transcriptional Activation and Repression Responses to Phosphate Starvation in Arabidopsis
title_short A Central Regulatory System Largely Controls Transcriptional Activation and Repression Responses to Phosphate Starvation in Arabidopsis
title_sort central regulatory system largely controls transcriptional activation and repression responses to phosphate starvation in arabidopsis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2936532/
https://www.ncbi.nlm.nih.gov/pubmed/20838596
http://dx.doi.org/10.1371/journal.pgen.1001102
work_keys_str_mv AT bustosregla acentralregulatorysystemlargelycontrolstranscriptionalactivationandrepressionresponsestophosphatestarvationinarabidopsis
AT castrillogabriel acentralregulatorysystemlargelycontrolstranscriptionalactivationandrepressionresponsestophosphatestarvationinarabidopsis
AT linharesfrancisco acentralregulatorysystemlargelycontrolstranscriptionalactivationandrepressionresponsestophosphatestarvationinarabidopsis
AT pugamariaisabel acentralregulatorysystemlargelycontrolstranscriptionalactivationandrepressionresponsestophosphatestarvationinarabidopsis
AT rubiovicente acentralregulatorysystemlargelycontrolstranscriptionalactivationandrepressionresponsestophosphatestarvationinarabidopsis
AT perezperezjulian acentralregulatorysystemlargelycontrolstranscriptionalactivationandrepressionresponsestophosphatestarvationinarabidopsis
AT solanoroberto acentralregulatorysystemlargelycontrolstranscriptionalactivationandrepressionresponsestophosphatestarvationinarabidopsis
AT leyvaantonio acentralregulatorysystemlargelycontrolstranscriptionalactivationandrepressionresponsestophosphatestarvationinarabidopsis
AT pazaresjavier acentralregulatorysystemlargelycontrolstranscriptionalactivationandrepressionresponsestophosphatestarvationinarabidopsis
AT bustosregla centralregulatorysystemlargelycontrolstranscriptionalactivationandrepressionresponsestophosphatestarvationinarabidopsis
AT castrillogabriel centralregulatorysystemlargelycontrolstranscriptionalactivationandrepressionresponsestophosphatestarvationinarabidopsis
AT linharesfrancisco centralregulatorysystemlargelycontrolstranscriptionalactivationandrepressionresponsestophosphatestarvationinarabidopsis
AT pugamariaisabel centralregulatorysystemlargelycontrolstranscriptionalactivationandrepressionresponsestophosphatestarvationinarabidopsis
AT rubiovicente centralregulatorysystemlargelycontrolstranscriptionalactivationandrepressionresponsestophosphatestarvationinarabidopsis
AT perezperezjulian centralregulatorysystemlargelycontrolstranscriptionalactivationandrepressionresponsestophosphatestarvationinarabidopsis
AT solanoroberto centralregulatorysystemlargelycontrolstranscriptionalactivationandrepressionresponsestophosphatestarvationinarabidopsis
AT leyvaantonio centralregulatorysystemlargelycontrolstranscriptionalactivationandrepressionresponsestophosphatestarvationinarabidopsis
AT pazaresjavier centralregulatorysystemlargelycontrolstranscriptionalactivationandrepressionresponsestophosphatestarvationinarabidopsis