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Probing Native Protein Structures by Chemical Cross-linking, Mass Spectrometry, and Bioinformatics
Chemical cross-linking of reactive groups in native proteins and protein complexes in combination with the identification of cross-linked sites by mass spectrometry has been in use for more than a decade. Recent advances in instrumentation, cross-linking protocols, and analysis software have led to...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The American Society for Biochemistry and Molecular Biology
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938055/ https://www.ncbi.nlm.nih.gov/pubmed/20360032 http://dx.doi.org/10.1074/mcp.R000001-MCP201 |
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author | Leitner, Alexander Walzthoeni, Thomas Kahraman, Abdullah Herzog, Franz Rinner, Oliver Beck, Martin Aebersold, Ruedi |
author_facet | Leitner, Alexander Walzthoeni, Thomas Kahraman, Abdullah Herzog, Franz Rinner, Oliver Beck, Martin Aebersold, Ruedi |
author_sort | Leitner, Alexander |
collection | PubMed |
description | Chemical cross-linking of reactive groups in native proteins and protein complexes in combination with the identification of cross-linked sites by mass spectrometry has been in use for more than a decade. Recent advances in instrumentation, cross-linking protocols, and analysis software have led to a renewed interest in this technique, which promises to provide important information about native protein structure and the topology of protein complexes. In this article, we discuss the critical steps of chemical cross-linking and its implications for (structural) biology: reagent design and cross-linking protocols, separation and mass spectrometric analysis of cross-linked samples, dedicated software for data analysis, and the use of cross-linking data for computational modeling. Finally, the impact of protein cross-linking on various biological disciplines is highlighted. |
format | Text |
id | pubmed-2938055 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | The American Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-29380552010-09-17 Probing Native Protein Structures by Chemical Cross-linking, Mass Spectrometry, and Bioinformatics Leitner, Alexander Walzthoeni, Thomas Kahraman, Abdullah Herzog, Franz Rinner, Oliver Beck, Martin Aebersold, Ruedi Mol Cell Proteomics Review Chemical cross-linking of reactive groups in native proteins and protein complexes in combination with the identification of cross-linked sites by mass spectrometry has been in use for more than a decade. Recent advances in instrumentation, cross-linking protocols, and analysis software have led to a renewed interest in this technique, which promises to provide important information about native protein structure and the topology of protein complexes. In this article, we discuss the critical steps of chemical cross-linking and its implications for (structural) biology: reagent design and cross-linking protocols, separation and mass spectrometric analysis of cross-linked samples, dedicated software for data analysis, and the use of cross-linking data for computational modeling. Finally, the impact of protein cross-linking on various biological disciplines is highlighted. The American Society for Biochemistry and Molecular Biology 2010-08 2010-03-31 /pmc/articles/PMC2938055/ /pubmed/20360032 http://dx.doi.org/10.1074/mcp.R000001-MCP201 Text en © 2010 by The American Society for Biochemistry and Molecular Biology, Inc. Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) applies to Author Choice Articles |
spellingShingle | Review Leitner, Alexander Walzthoeni, Thomas Kahraman, Abdullah Herzog, Franz Rinner, Oliver Beck, Martin Aebersold, Ruedi Probing Native Protein Structures by Chemical Cross-linking, Mass Spectrometry, and Bioinformatics |
title | Probing Native Protein Structures by Chemical Cross-linking, Mass Spectrometry, and Bioinformatics |
title_full | Probing Native Protein Structures by Chemical Cross-linking, Mass Spectrometry, and Bioinformatics |
title_fullStr | Probing Native Protein Structures by Chemical Cross-linking, Mass Spectrometry, and Bioinformatics |
title_full_unstemmed | Probing Native Protein Structures by Chemical Cross-linking, Mass Spectrometry, and Bioinformatics |
title_short | Probing Native Protein Structures by Chemical Cross-linking, Mass Spectrometry, and Bioinformatics |
title_sort | probing native protein structures by chemical cross-linking, mass spectrometry, and bioinformatics |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938055/ https://www.ncbi.nlm.nih.gov/pubmed/20360032 http://dx.doi.org/10.1074/mcp.R000001-MCP201 |
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