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ERAL1 is associated with mitochondrial ribosome and elimination of ERAL1 leads to mitochondrial dysfunction and growth retardation

ERAL1, a homologue of Era protein in Escherichia coli, is a member of conserved GTP-binding proteins with RNA-binding activity. Depletion of prokaryotic Era inhibits cell division without affecting chromosome segregation. Previously, we isolated ERAL1 protein as one of proteins which were associated...

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Autores principales: Uchiumi, Takeshi, Ohgaki, Kippei, Yagi, Mikako, Aoki, Yoshimasa, Sakai, Aya, Matsumoto, Shinya, Kang, Dongchon
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938226/
https://www.ncbi.nlm.nih.gov/pubmed/20430825
http://dx.doi.org/10.1093/nar/gkq305
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author Uchiumi, Takeshi
Ohgaki, Kippei
Yagi, Mikako
Aoki, Yoshimasa
Sakai, Aya
Matsumoto, Shinya
Kang, Dongchon
author_facet Uchiumi, Takeshi
Ohgaki, Kippei
Yagi, Mikako
Aoki, Yoshimasa
Sakai, Aya
Matsumoto, Shinya
Kang, Dongchon
author_sort Uchiumi, Takeshi
collection PubMed
description ERAL1, a homologue of Era protein in Escherichia coli, is a member of conserved GTP-binding proteins with RNA-binding activity. Depletion of prokaryotic Era inhibits cell division without affecting chromosome segregation. Previously, we isolated ERAL1 protein as one of proteins which were associated with mitochondrial transcription factor A by using immunoprecipitation. In this study, we analysed the localization and function of ERAL1 in mammalian cells. ERAL1 was localized in mitochondrial matrix and associated with mitoribosomal proteins including the 12S rRNA. siRNA knockdown of ERAL1 decreased mitochondrial translation, caused redistribution of ribosomal small subunits and reduced 12S rRNA. The knockdown of ERAL1 in human HeLa cells elevated mitochondrial superoxide production and slightly decreased mitochondrial membrane potential. The knockdown inhibited the growth of HeLa cells with an accumulation of apoptotic cells. These results suggest that ERAL1 is localized in a small subunit of the mitochondrial ribosome, plays an important role in the small ribosomal constitution, and is also involved in cell viability.
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spelling pubmed-29382262010-09-13 ERAL1 is associated with mitochondrial ribosome and elimination of ERAL1 leads to mitochondrial dysfunction and growth retardation Uchiumi, Takeshi Ohgaki, Kippei Yagi, Mikako Aoki, Yoshimasa Sakai, Aya Matsumoto, Shinya Kang, Dongchon Nucleic Acids Res RNA ERAL1, a homologue of Era protein in Escherichia coli, is a member of conserved GTP-binding proteins with RNA-binding activity. Depletion of prokaryotic Era inhibits cell division without affecting chromosome segregation. Previously, we isolated ERAL1 protein as one of proteins which were associated with mitochondrial transcription factor A by using immunoprecipitation. In this study, we analysed the localization and function of ERAL1 in mammalian cells. ERAL1 was localized in mitochondrial matrix and associated with mitoribosomal proteins including the 12S rRNA. siRNA knockdown of ERAL1 decreased mitochondrial translation, caused redistribution of ribosomal small subunits and reduced 12S rRNA. The knockdown of ERAL1 in human HeLa cells elevated mitochondrial superoxide production and slightly decreased mitochondrial membrane potential. The knockdown inhibited the growth of HeLa cells with an accumulation of apoptotic cells. These results suggest that ERAL1 is localized in a small subunit of the mitochondrial ribosome, plays an important role in the small ribosomal constitution, and is also involved in cell viability. Oxford University Press 2010-09 2010-04-29 /pmc/articles/PMC2938226/ /pubmed/20430825 http://dx.doi.org/10.1093/nar/gkq305 Text en © The Author(s) 2010. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/2.5 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle RNA
Uchiumi, Takeshi
Ohgaki, Kippei
Yagi, Mikako
Aoki, Yoshimasa
Sakai, Aya
Matsumoto, Shinya
Kang, Dongchon
ERAL1 is associated with mitochondrial ribosome and elimination of ERAL1 leads to mitochondrial dysfunction and growth retardation
title ERAL1 is associated with mitochondrial ribosome and elimination of ERAL1 leads to mitochondrial dysfunction and growth retardation
title_full ERAL1 is associated with mitochondrial ribosome and elimination of ERAL1 leads to mitochondrial dysfunction and growth retardation
title_fullStr ERAL1 is associated with mitochondrial ribosome and elimination of ERAL1 leads to mitochondrial dysfunction and growth retardation
title_full_unstemmed ERAL1 is associated with mitochondrial ribosome and elimination of ERAL1 leads to mitochondrial dysfunction and growth retardation
title_short ERAL1 is associated with mitochondrial ribosome and elimination of ERAL1 leads to mitochondrial dysfunction and growth retardation
title_sort eral1 is associated with mitochondrial ribosome and elimination of eral1 leads to mitochondrial dysfunction and growth retardation
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938226/
https://www.ncbi.nlm.nih.gov/pubmed/20430825
http://dx.doi.org/10.1093/nar/gkq305
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