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KHDC1B Is a Novel CPEB Binding Partner Specifically Expressed in Mouse Oocytes and Early Embryos

mRNAs required for meiotic maturation and early embryonic development are stored in growing oocytes. These transcripts are translationally repressed until hormonal cues trigger ovulation. Errors in translation underlie some cases of human infertility and are associated with ovarian germ cell tumors....

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Detalles Bibliográficos
Autores principales: Cai, Congli, Tamai, Keiko, Molyneaux, Kathleen
Formato: Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938380/
https://www.ncbi.nlm.nih.gov/pubmed/20668163
http://dx.doi.org/10.1091/mbc.E10-03-0255
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author Cai, Congli
Tamai, Keiko
Molyneaux, Kathleen
author_facet Cai, Congli
Tamai, Keiko
Molyneaux, Kathleen
author_sort Cai, Congli
collection PubMed
description mRNAs required for meiotic maturation and early embryonic development are stored in growing oocytes. These transcripts are translationally repressed until hormonal cues trigger ovulation. Errors in translation underlie some cases of human infertility and are associated with ovarian germ cell tumors. However, it remains unclear how maternal transcripts are kept quiescent in mammals. This study describes a potential translational regulator, KHDC1B. KHDC1B is a member of a small family of KH-domain containing proteins specific to eutherian mammals. Two family members, KHDC1A and 1B, are highly expressed in oocytes. KHDC1A and 1B bind polyU agarose and form oligomers like other KH-domain proteins. The functions of these proteins were tested by expression in Xenopus embryos. KHDC1A caused cell death, whereas KHDC1B caused cleavage arrest. This arrest phenotype was rescued by coexpression of the mouse translational regulator cytoplasmic polyadenylation binding protein 1 (mCPEB1). Coimmunoprecipitation and coimmunostaining experiments confirmed the functional interaction between KHDC1B and mCPEB1. Finally, KHDC1B levels and binding partners were shown to fluctuate with the cell cycle. KHDC1B, via its interaction with mCEPB1, may regulate translation of mRNA targets required for oocyte maturation.
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spelling pubmed-29383802010-11-30 KHDC1B Is a Novel CPEB Binding Partner Specifically Expressed in Mouse Oocytes and Early Embryos Cai, Congli Tamai, Keiko Molyneaux, Kathleen Mol Biol Cell Articles mRNAs required for meiotic maturation and early embryonic development are stored in growing oocytes. These transcripts are translationally repressed until hormonal cues trigger ovulation. Errors in translation underlie some cases of human infertility and are associated with ovarian germ cell tumors. However, it remains unclear how maternal transcripts are kept quiescent in mammals. This study describes a potential translational regulator, KHDC1B. KHDC1B is a member of a small family of KH-domain containing proteins specific to eutherian mammals. Two family members, KHDC1A and 1B, are highly expressed in oocytes. KHDC1A and 1B bind polyU agarose and form oligomers like other KH-domain proteins. The functions of these proteins were tested by expression in Xenopus embryos. KHDC1A caused cell death, whereas KHDC1B caused cleavage arrest. This arrest phenotype was rescued by coexpression of the mouse translational regulator cytoplasmic polyadenylation binding protein 1 (mCPEB1). Coimmunoprecipitation and coimmunostaining experiments confirmed the functional interaction between KHDC1B and mCPEB1. Finally, KHDC1B levels and binding partners were shown to fluctuate with the cell cycle. KHDC1B, via its interaction with mCEPB1, may regulate translation of mRNA targets required for oocyte maturation. The American Society for Cell Biology 2010-09-15 /pmc/articles/PMC2938380/ /pubmed/20668163 http://dx.doi.org/10.1091/mbc.E10-03-0255 Text en © 2010 by The American Society for Cell Biology This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).
spellingShingle Articles
Cai, Congli
Tamai, Keiko
Molyneaux, Kathleen
KHDC1B Is a Novel CPEB Binding Partner Specifically Expressed in Mouse Oocytes and Early Embryos
title KHDC1B Is a Novel CPEB Binding Partner Specifically Expressed in Mouse Oocytes and Early Embryos
title_full KHDC1B Is a Novel CPEB Binding Partner Specifically Expressed in Mouse Oocytes and Early Embryos
title_fullStr KHDC1B Is a Novel CPEB Binding Partner Specifically Expressed in Mouse Oocytes and Early Embryos
title_full_unstemmed KHDC1B Is a Novel CPEB Binding Partner Specifically Expressed in Mouse Oocytes and Early Embryos
title_short KHDC1B Is a Novel CPEB Binding Partner Specifically Expressed in Mouse Oocytes and Early Embryos
title_sort khdc1b is a novel cpeb binding partner specifically expressed in mouse oocytes and early embryos
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938380/
https://www.ncbi.nlm.nih.gov/pubmed/20668163
http://dx.doi.org/10.1091/mbc.E10-03-0255
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