Cargando…

Cortical Mechanics and Meiosis II Completion in Mammalian Oocytes Are Mediated by Myosin-II and Ezrin-Radixin-Moesin (ERM) Proteins

Cell division is inherently mechanical, with cell mechanics being a critical determinant governing the cell shape changes that accompany progression through the cell cycle. The mechanical properties of symmetrically dividing mitotic cells have been well characterized, whereas the contribution of cel...

Descripción completa

Detalles Bibliográficos
Autores principales: Larson, Stephanie M., Lee, Hyo J., Hung, Pei-hsuan, Matthews, Lauren M., Robinson, Douglas N., Evans, Janice P.
Formato: Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938384/
https://www.ncbi.nlm.nih.gov/pubmed/20660156
http://dx.doi.org/10.1091/mbc.E10-01-0066
_version_ 1782186605668204544
author Larson, Stephanie M.
Lee, Hyo J.
Hung, Pei-hsuan
Matthews, Lauren M.
Robinson, Douglas N.
Evans, Janice P.
author_facet Larson, Stephanie M.
Lee, Hyo J.
Hung, Pei-hsuan
Matthews, Lauren M.
Robinson, Douglas N.
Evans, Janice P.
author_sort Larson, Stephanie M.
collection PubMed
description Cell division is inherently mechanical, with cell mechanics being a critical determinant governing the cell shape changes that accompany progression through the cell cycle. The mechanical properties of symmetrically dividing mitotic cells have been well characterized, whereas the contribution of cellular mechanics to the strikingly asymmetric divisions of female meiosis is very poorly understood. Progression of the mammalian oocyte through meiosis involves remodeling of the cortex and proper orientation of the meiotic spindle, and thus we hypothesized that cortical tension and stiffness would change through meiotic maturation and fertilization to facilitate and/or direct cellular remodeling. This work shows that tension in mouse oocytes drops about sixfold during meiotic maturation from prophase I to metaphase II and then increases ∼1.6-fold upon fertilization. The metaphase II egg is polarized, with tension differing ∼2.5-fold between the cortex over the meiotic spindle and the opposite cortex, suggesting that meiotic maturation is accompanied by assembly of a cortical domain with stiffer mechanics as part of the process to achieve asymmetric cytokinesis. We further demonstrate that actin, myosin-II, and the ERM (Ezrin/Radixin/Moesin) family of proteins are enriched in complementary cortical domains and mediate cellular mechanics in mammalian eggs. Manipulation of actin, myosin-II, and ERM function alters tension levels and also is associated with dramatic spindle abnormalities with completion of meiosis II after fertilization. Thus, myosin-II and ERM proteins modulate mechanical properties in oocytes, contributing to cell polarity and to completion of meiosis.
format Text
id pubmed-2938384
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher The American Society for Cell Biology
record_format MEDLINE/PubMed
spelling pubmed-29383842010-11-30 Cortical Mechanics and Meiosis II Completion in Mammalian Oocytes Are Mediated by Myosin-II and Ezrin-Radixin-Moesin (ERM) Proteins Larson, Stephanie M. Lee, Hyo J. Hung, Pei-hsuan Matthews, Lauren M. Robinson, Douglas N. Evans, Janice P. Mol Biol Cell Articles Cell division is inherently mechanical, with cell mechanics being a critical determinant governing the cell shape changes that accompany progression through the cell cycle. The mechanical properties of symmetrically dividing mitotic cells have been well characterized, whereas the contribution of cellular mechanics to the strikingly asymmetric divisions of female meiosis is very poorly understood. Progression of the mammalian oocyte through meiosis involves remodeling of the cortex and proper orientation of the meiotic spindle, and thus we hypothesized that cortical tension and stiffness would change through meiotic maturation and fertilization to facilitate and/or direct cellular remodeling. This work shows that tension in mouse oocytes drops about sixfold during meiotic maturation from prophase I to metaphase II and then increases ∼1.6-fold upon fertilization. The metaphase II egg is polarized, with tension differing ∼2.5-fold between the cortex over the meiotic spindle and the opposite cortex, suggesting that meiotic maturation is accompanied by assembly of a cortical domain with stiffer mechanics as part of the process to achieve asymmetric cytokinesis. We further demonstrate that actin, myosin-II, and the ERM (Ezrin/Radixin/Moesin) family of proteins are enriched in complementary cortical domains and mediate cellular mechanics in mammalian eggs. Manipulation of actin, myosin-II, and ERM function alters tension levels and also is associated with dramatic spindle abnormalities with completion of meiosis II after fertilization. Thus, myosin-II and ERM proteins modulate mechanical properties in oocytes, contributing to cell polarity and to completion of meiosis. The American Society for Cell Biology 2010-09-15 /pmc/articles/PMC2938384/ /pubmed/20660156 http://dx.doi.org/10.1091/mbc.E10-01-0066 Text en © 2010 by The American Society for Cell Biology This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).
spellingShingle Articles
Larson, Stephanie M.
Lee, Hyo J.
Hung, Pei-hsuan
Matthews, Lauren M.
Robinson, Douglas N.
Evans, Janice P.
Cortical Mechanics and Meiosis II Completion in Mammalian Oocytes Are Mediated by Myosin-II and Ezrin-Radixin-Moesin (ERM) Proteins
title Cortical Mechanics and Meiosis II Completion in Mammalian Oocytes Are Mediated by Myosin-II and Ezrin-Radixin-Moesin (ERM) Proteins
title_full Cortical Mechanics and Meiosis II Completion in Mammalian Oocytes Are Mediated by Myosin-II and Ezrin-Radixin-Moesin (ERM) Proteins
title_fullStr Cortical Mechanics and Meiosis II Completion in Mammalian Oocytes Are Mediated by Myosin-II and Ezrin-Radixin-Moesin (ERM) Proteins
title_full_unstemmed Cortical Mechanics and Meiosis II Completion in Mammalian Oocytes Are Mediated by Myosin-II and Ezrin-Radixin-Moesin (ERM) Proteins
title_short Cortical Mechanics and Meiosis II Completion in Mammalian Oocytes Are Mediated by Myosin-II and Ezrin-Radixin-Moesin (ERM) Proteins
title_sort cortical mechanics and meiosis ii completion in mammalian oocytes are mediated by myosin-ii and ezrin-radixin-moesin (erm) proteins
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938384/
https://www.ncbi.nlm.nih.gov/pubmed/20660156
http://dx.doi.org/10.1091/mbc.E10-01-0066
work_keys_str_mv AT larsonstephaniem corticalmechanicsandmeiosisiicompletioninmammalianoocytesaremediatedbymyosiniiandezrinradixinmoesinermproteins
AT leehyoj corticalmechanicsandmeiosisiicompletioninmammalianoocytesaremediatedbymyosiniiandezrinradixinmoesinermproteins
AT hungpeihsuan corticalmechanicsandmeiosisiicompletioninmammalianoocytesaremediatedbymyosiniiandezrinradixinmoesinermproteins
AT matthewslaurenm corticalmechanicsandmeiosisiicompletioninmammalianoocytesaremediatedbymyosiniiandezrinradixinmoesinermproteins
AT robinsondouglasn corticalmechanicsandmeiosisiicompletioninmammalianoocytesaremediatedbymyosiniiandezrinradixinmoesinermproteins
AT evansjanicep corticalmechanicsandmeiosisiicompletioninmammalianoocytesaremediatedbymyosiniiandezrinradixinmoesinermproteins