Cargando…

Rho and Anillin-dependent Control of mDia2 Localization and Function in Cytokinesis

Diaphanous-related formin, mDia, is an actin nucleation/polymerization factor functioning downstream of the small GTPase Rho. Although Rho is critically involved in cytokinesis, it remains elusive how Rho effectors and other regulators of cytoskeletons work together to accomplish this process. Here...

Descripción completa

Detalles Bibliográficos
Autores principales: Watanabe, Sadanori, Okawa, Katsuya, Miki, Takashi, Sakamoto, Satoko, Morinaga, Tomoko, Segawa, Kohei, Arakawa, Takatoshi, Kinoshita, Makoto, Ishizaki, Toshimasa, Narumiya, Shuh
Formato: Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938385/
https://www.ncbi.nlm.nih.gov/pubmed/20660154
http://dx.doi.org/10.1091/mbc.E10-04-0324
_version_ 1782186605906231296
author Watanabe, Sadanori
Okawa, Katsuya
Miki, Takashi
Sakamoto, Satoko
Morinaga, Tomoko
Segawa, Kohei
Arakawa, Takatoshi
Kinoshita, Makoto
Ishizaki, Toshimasa
Narumiya, Shuh
author_facet Watanabe, Sadanori
Okawa, Katsuya
Miki, Takashi
Sakamoto, Satoko
Morinaga, Tomoko
Segawa, Kohei
Arakawa, Takatoshi
Kinoshita, Makoto
Ishizaki, Toshimasa
Narumiya, Shuh
author_sort Watanabe, Sadanori
collection PubMed
description Diaphanous-related formin, mDia, is an actin nucleation/polymerization factor functioning downstream of the small GTPase Rho. Although Rho is critically involved in cytokinesis, it remains elusive how Rho effectors and other regulators of cytoskeletons work together to accomplish this process. Here we focused on mDia2, an mDia isoform involved in cytokinesis of NIH 3T3 cells, and analyzed mechanisms of its localization in cytokinesis. We found that targeting of mDia2 to the cleavage furrow requires not only its binding to RhoA but also its diaphanous-inhibitory domain (DID). We then performed pulldown assays using a fragment containing the latter domain as a bait and identified anillin as a novel mDia2 interaction partner. The anillin-binding is competitive with the diaphanous autoregulatory domain (DAD) of mDia2 in its autoinhibitory interaction. A series of RNA interference and functional rescue experiments has revealed that, in addition to the Rho GTPase-mediated activation, the interaction between mDia2 and anillin is required for the localization and function of mDia2 in cytokinesis.
format Text
id pubmed-2938385
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher The American Society for Cell Biology
record_format MEDLINE/PubMed
spelling pubmed-29383852010-11-30 Rho and Anillin-dependent Control of mDia2 Localization and Function in Cytokinesis Watanabe, Sadanori Okawa, Katsuya Miki, Takashi Sakamoto, Satoko Morinaga, Tomoko Segawa, Kohei Arakawa, Takatoshi Kinoshita, Makoto Ishizaki, Toshimasa Narumiya, Shuh Mol Biol Cell Articles Diaphanous-related formin, mDia, is an actin nucleation/polymerization factor functioning downstream of the small GTPase Rho. Although Rho is critically involved in cytokinesis, it remains elusive how Rho effectors and other regulators of cytoskeletons work together to accomplish this process. Here we focused on mDia2, an mDia isoform involved in cytokinesis of NIH 3T3 cells, and analyzed mechanisms of its localization in cytokinesis. We found that targeting of mDia2 to the cleavage furrow requires not only its binding to RhoA but also its diaphanous-inhibitory domain (DID). We then performed pulldown assays using a fragment containing the latter domain as a bait and identified anillin as a novel mDia2 interaction partner. The anillin-binding is competitive with the diaphanous autoregulatory domain (DAD) of mDia2 in its autoinhibitory interaction. A series of RNA interference and functional rescue experiments has revealed that, in addition to the Rho GTPase-mediated activation, the interaction between mDia2 and anillin is required for the localization and function of mDia2 in cytokinesis. The American Society for Cell Biology 2010-09-15 /pmc/articles/PMC2938385/ /pubmed/20660154 http://dx.doi.org/10.1091/mbc.E10-04-0324 Text en © 2010 by The American Society for Cell Biology This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).
spellingShingle Articles
Watanabe, Sadanori
Okawa, Katsuya
Miki, Takashi
Sakamoto, Satoko
Morinaga, Tomoko
Segawa, Kohei
Arakawa, Takatoshi
Kinoshita, Makoto
Ishizaki, Toshimasa
Narumiya, Shuh
Rho and Anillin-dependent Control of mDia2 Localization and Function in Cytokinesis
title Rho and Anillin-dependent Control of mDia2 Localization and Function in Cytokinesis
title_full Rho and Anillin-dependent Control of mDia2 Localization and Function in Cytokinesis
title_fullStr Rho and Anillin-dependent Control of mDia2 Localization and Function in Cytokinesis
title_full_unstemmed Rho and Anillin-dependent Control of mDia2 Localization and Function in Cytokinesis
title_short Rho and Anillin-dependent Control of mDia2 Localization and Function in Cytokinesis
title_sort rho and anillin-dependent control of mdia2 localization and function in cytokinesis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938385/
https://www.ncbi.nlm.nih.gov/pubmed/20660154
http://dx.doi.org/10.1091/mbc.E10-04-0324
work_keys_str_mv AT watanabesadanori rhoandanillindependentcontrolofmdia2localizationandfunctionincytokinesis
AT okawakatsuya rhoandanillindependentcontrolofmdia2localizationandfunctionincytokinesis
AT mikitakashi rhoandanillindependentcontrolofmdia2localizationandfunctionincytokinesis
AT sakamotosatoko rhoandanillindependentcontrolofmdia2localizationandfunctionincytokinesis
AT morinagatomoko rhoandanillindependentcontrolofmdia2localizationandfunctionincytokinesis
AT segawakohei rhoandanillindependentcontrolofmdia2localizationandfunctionincytokinesis
AT arakawatakatoshi rhoandanillindependentcontrolofmdia2localizationandfunctionincytokinesis
AT kinoshitamakoto rhoandanillindependentcontrolofmdia2localizationandfunctionincytokinesis
AT ishizakitoshimasa rhoandanillindependentcontrolofmdia2localizationandfunctionincytokinesis
AT narumiyashuh rhoandanillindependentcontrolofmdia2localizationandfunctionincytokinesis