Cargando…
Application and Validation of PFGE for Serovar Identification of Leptospira Clinical Isolates
Serovar identification of clinical isolates of Leptospira is generally not performed on a routine basis, yet the identity of an infecting serovar is valuable from both epidemiologic and public health standpoints. Only a small number of reference laboratories worldwide have the capability to perform...
Autores principales: | , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2939049/ https://www.ncbi.nlm.nih.gov/pubmed/20856859 http://dx.doi.org/10.1371/journal.pntd.0000824 |
_version_ | 1782186698732470272 |
---|---|
author | Galloway, Renee L. Levett, Paul N. |
author_facet | Galloway, Renee L. Levett, Paul N. |
author_sort | Galloway, Renee L. |
collection | PubMed |
description | Serovar identification of clinical isolates of Leptospira is generally not performed on a routine basis, yet the identity of an infecting serovar is valuable from both epidemiologic and public health standpoints. Only a small number of reference laboratories worldwide have the capability to perform the cross agglutinin absorption test (CAAT), the reference method for serovar identification. Pulsed-field gel electrophoresis (PFGE) is an alternative method to CAAT that facilitates rapid identification of leptospires to the serovar level. We employed PFGE to evaluate 175 isolates obtained from humans and animals submitted to the Centers for Disease Control and Prevention (CDC) between 1993 and 2007. PFGE patterns for each isolate were generated using the NotI restriction enzyme and compared to a reference database consisting of more than 200 reference strains. Of the 175 clinical isolates evaluated, 136 (78%) were identified to the serovar level by the database, and an additional 27 isolates (15%) have been identified as probable new serovars. The remaining isolates yet to be identified are either not represented in the database or require further study to determine whether or not they also represent new serovars. PFGE proved to be a useful tool for serovar identification of clinical isolates of known serovars from different geographic regions and a variety of different hosts and for recognizing potential new serovars. |
format | Text |
id | pubmed-2939049 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-29390492010-09-20 Application and Validation of PFGE for Serovar Identification of Leptospira Clinical Isolates Galloway, Renee L. Levett, Paul N. PLoS Negl Trop Dis Research Article Serovar identification of clinical isolates of Leptospira is generally not performed on a routine basis, yet the identity of an infecting serovar is valuable from both epidemiologic and public health standpoints. Only a small number of reference laboratories worldwide have the capability to perform the cross agglutinin absorption test (CAAT), the reference method for serovar identification. Pulsed-field gel electrophoresis (PFGE) is an alternative method to CAAT that facilitates rapid identification of leptospires to the serovar level. We employed PFGE to evaluate 175 isolates obtained from humans and animals submitted to the Centers for Disease Control and Prevention (CDC) between 1993 and 2007. PFGE patterns for each isolate were generated using the NotI restriction enzyme and compared to a reference database consisting of more than 200 reference strains. Of the 175 clinical isolates evaluated, 136 (78%) were identified to the serovar level by the database, and an additional 27 isolates (15%) have been identified as probable new serovars. The remaining isolates yet to be identified are either not represented in the database or require further study to determine whether or not they also represent new serovars. PFGE proved to be a useful tool for serovar identification of clinical isolates of known serovars from different geographic regions and a variety of different hosts and for recognizing potential new serovars. Public Library of Science 2010-09-14 /pmc/articles/PMC2939049/ /pubmed/20856859 http://dx.doi.org/10.1371/journal.pntd.0000824 Text en This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. |
spellingShingle | Research Article Galloway, Renee L. Levett, Paul N. Application and Validation of PFGE for Serovar Identification of Leptospira Clinical Isolates |
title | Application and Validation of PFGE for Serovar Identification of Leptospira Clinical Isolates |
title_full | Application and Validation of PFGE for Serovar Identification of Leptospira Clinical Isolates |
title_fullStr | Application and Validation of PFGE for Serovar Identification of Leptospira Clinical Isolates |
title_full_unstemmed | Application and Validation of PFGE for Serovar Identification of Leptospira Clinical Isolates |
title_short | Application and Validation of PFGE for Serovar Identification of Leptospira Clinical Isolates |
title_sort | application and validation of pfge for serovar identification of leptospira clinical isolates |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2939049/ https://www.ncbi.nlm.nih.gov/pubmed/20856859 http://dx.doi.org/10.1371/journal.pntd.0000824 |
work_keys_str_mv | AT gallowayreneel applicationandvalidationofpfgeforserovaridentificationofleptospiraclinicalisolates AT levettpauln applicationandvalidationofpfgeforserovaridentificationofleptospiraclinicalisolates |