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Application and Validation of PFGE for Serovar Identification of Leptospira Clinical Isolates

Serovar identification of clinical isolates of Leptospira is generally not performed on a routine basis, yet the identity of an infecting serovar is valuable from both epidemiologic and public health standpoints. Only a small number of reference laboratories worldwide have the capability to perform...

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Autores principales: Galloway, Renee L., Levett, Paul N.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2939049/
https://www.ncbi.nlm.nih.gov/pubmed/20856859
http://dx.doi.org/10.1371/journal.pntd.0000824
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author Galloway, Renee L.
Levett, Paul N.
author_facet Galloway, Renee L.
Levett, Paul N.
author_sort Galloway, Renee L.
collection PubMed
description Serovar identification of clinical isolates of Leptospira is generally not performed on a routine basis, yet the identity of an infecting serovar is valuable from both epidemiologic and public health standpoints. Only a small number of reference laboratories worldwide have the capability to perform the cross agglutinin absorption test (CAAT), the reference method for serovar identification. Pulsed-field gel electrophoresis (PFGE) is an alternative method to CAAT that facilitates rapid identification of leptospires to the serovar level. We employed PFGE to evaluate 175 isolates obtained from humans and animals submitted to the Centers for Disease Control and Prevention (CDC) between 1993 and 2007. PFGE patterns for each isolate were generated using the NotI restriction enzyme and compared to a reference database consisting of more than 200 reference strains. Of the 175 clinical isolates evaluated, 136 (78%) were identified to the serovar level by the database, and an additional 27 isolates (15%) have been identified as probable new serovars. The remaining isolates yet to be identified are either not represented in the database or require further study to determine whether or not they also represent new serovars. PFGE proved to be a useful tool for serovar identification of clinical isolates of known serovars from different geographic regions and a variety of different hosts and for recognizing potential new serovars.
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spelling pubmed-29390492010-09-20 Application and Validation of PFGE for Serovar Identification of Leptospira Clinical Isolates Galloway, Renee L. Levett, Paul N. PLoS Negl Trop Dis Research Article Serovar identification of clinical isolates of Leptospira is generally not performed on a routine basis, yet the identity of an infecting serovar is valuable from both epidemiologic and public health standpoints. Only a small number of reference laboratories worldwide have the capability to perform the cross agglutinin absorption test (CAAT), the reference method for serovar identification. Pulsed-field gel electrophoresis (PFGE) is an alternative method to CAAT that facilitates rapid identification of leptospires to the serovar level. We employed PFGE to evaluate 175 isolates obtained from humans and animals submitted to the Centers for Disease Control and Prevention (CDC) between 1993 and 2007. PFGE patterns for each isolate were generated using the NotI restriction enzyme and compared to a reference database consisting of more than 200 reference strains. Of the 175 clinical isolates evaluated, 136 (78%) were identified to the serovar level by the database, and an additional 27 isolates (15%) have been identified as probable new serovars. The remaining isolates yet to be identified are either not represented in the database or require further study to determine whether or not they also represent new serovars. PFGE proved to be a useful tool for serovar identification of clinical isolates of known serovars from different geographic regions and a variety of different hosts and for recognizing potential new serovars. Public Library of Science 2010-09-14 /pmc/articles/PMC2939049/ /pubmed/20856859 http://dx.doi.org/10.1371/journal.pntd.0000824 Text en This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Galloway, Renee L.
Levett, Paul N.
Application and Validation of PFGE for Serovar Identification of Leptospira Clinical Isolates
title Application and Validation of PFGE for Serovar Identification of Leptospira Clinical Isolates
title_full Application and Validation of PFGE for Serovar Identification of Leptospira Clinical Isolates
title_fullStr Application and Validation of PFGE for Serovar Identification of Leptospira Clinical Isolates
title_full_unstemmed Application and Validation of PFGE for Serovar Identification of Leptospira Clinical Isolates
title_short Application and Validation of PFGE for Serovar Identification of Leptospira Clinical Isolates
title_sort application and validation of pfge for serovar identification of leptospira clinical isolates
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2939049/
https://www.ncbi.nlm.nih.gov/pubmed/20856859
http://dx.doi.org/10.1371/journal.pntd.0000824
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