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Maggot Excretions Inhibit Biofilm Formation on Biomaterials

BACKGROUND: Biofilm-associated infections in trauma surgery are difficult to treat with conventional therapies. Therefore, it is important to develop new treatment modalities. Maggots in captured bags, which are permeable for larval excretions/secretions, aid in healing severe, infected wounds, susp...

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Autores principales: Cazander, Gwendolyn, van de Veerdonk, Mariëlle C., Vandenbroucke-Grauls, Christina M. J. E., Schreurs, Marco W. J., Jukema, Gerrolt N.
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2939353/
https://www.ncbi.nlm.nih.gov/pubmed/20309656
http://dx.doi.org/10.1007/s11999-010-1309-5
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author Cazander, Gwendolyn
van de Veerdonk, Mariëlle C.
Vandenbroucke-Grauls, Christina M. J. E.
Schreurs, Marco W. J.
Jukema, Gerrolt N.
author_facet Cazander, Gwendolyn
van de Veerdonk, Mariëlle C.
Vandenbroucke-Grauls, Christina M. J. E.
Schreurs, Marco W. J.
Jukema, Gerrolt N.
author_sort Cazander, Gwendolyn
collection PubMed
description BACKGROUND: Biofilm-associated infections in trauma surgery are difficult to treat with conventional therapies. Therefore, it is important to develop new treatment modalities. Maggots in captured bags, which are permeable for larval excretions/secretions, aid in healing severe, infected wounds, suspect for biofilm formation. Therefore we presumed maggot excretions/secretions would reduce biofilm formation. QUESTIONS/PURPOSES: We studied biofilm formation of Staphylococcus aureus, Staphylococcus epidermidis, Klebsiella oxytoca, Enterococcus faecalis, and Enterobacter cloacae on polyethylene, titanium, and stainless steel. We compared the quantities of biofilm formation between the bacterial species on the various biomaterials and the quantity of biofilm formation after various incubation times. Maggot excretions/secretions were added to existing biofilms to examine their effect. METHODS: Comb-like models of the biomaterials, made to fit in a 96-well microtiter plate, were incubated with bacterial suspension. The formed biofilms were stained in crystal violet, which was eluted in ethanol. The optical density (at 595 nm) of the eluate was determined to quantify biofilm formation. Maggot excretions/secretions were pipetted in different concentrations to (nonstained) 7-day-old biofilms, incubated 24 hours, and finally measured. RESULTS: The strongest biofilms were formed by S. aureus and S. epidermidis on polyethylene and the weakest on titanium. The highest quantity of biofilm formation was reached within 7 days for both bacteria. The presence of excretions/secretions reduced biofilm formation on all biomaterials. A maximum of 92% of biofilm reduction was measured. CONCLUSIONS: Our observations suggest maggot excretions/secretions decrease biofilm formation and could provide a new treatment for biofilm formation on infected biomaterials.
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spelling pubmed-29393532010-10-05 Maggot Excretions Inhibit Biofilm Formation on Biomaterials Cazander, Gwendolyn van de Veerdonk, Mariëlle C. Vandenbroucke-Grauls, Christina M. J. E. Schreurs, Marco W. J. Jukema, Gerrolt N. Clin Orthop Relat Res Basic Research BACKGROUND: Biofilm-associated infections in trauma surgery are difficult to treat with conventional therapies. Therefore, it is important to develop new treatment modalities. Maggots in captured bags, which are permeable for larval excretions/secretions, aid in healing severe, infected wounds, suspect for biofilm formation. Therefore we presumed maggot excretions/secretions would reduce biofilm formation. QUESTIONS/PURPOSES: We studied biofilm formation of Staphylococcus aureus, Staphylococcus epidermidis, Klebsiella oxytoca, Enterococcus faecalis, and Enterobacter cloacae on polyethylene, titanium, and stainless steel. We compared the quantities of biofilm formation between the bacterial species on the various biomaterials and the quantity of biofilm formation after various incubation times. Maggot excretions/secretions were added to existing biofilms to examine their effect. METHODS: Comb-like models of the biomaterials, made to fit in a 96-well microtiter plate, were incubated with bacterial suspension. The formed biofilms were stained in crystal violet, which was eluted in ethanol. The optical density (at 595 nm) of the eluate was determined to quantify biofilm formation. Maggot excretions/secretions were pipetted in different concentrations to (nonstained) 7-day-old biofilms, incubated 24 hours, and finally measured. RESULTS: The strongest biofilms were formed by S. aureus and S. epidermidis on polyethylene and the weakest on titanium. The highest quantity of biofilm formation was reached within 7 days for both bacteria. The presence of excretions/secretions reduced biofilm formation on all biomaterials. A maximum of 92% of biofilm reduction was measured. CONCLUSIONS: Our observations suggest maggot excretions/secretions decrease biofilm formation and could provide a new treatment for biofilm formation on infected biomaterials. Springer-Verlag 2010-03-23 2010-10 /pmc/articles/PMC2939353/ /pubmed/20309656 http://dx.doi.org/10.1007/s11999-010-1309-5 Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Basic Research
Cazander, Gwendolyn
van de Veerdonk, Mariëlle C.
Vandenbroucke-Grauls, Christina M. J. E.
Schreurs, Marco W. J.
Jukema, Gerrolt N.
Maggot Excretions Inhibit Biofilm Formation on Biomaterials
title Maggot Excretions Inhibit Biofilm Formation on Biomaterials
title_full Maggot Excretions Inhibit Biofilm Formation on Biomaterials
title_fullStr Maggot Excretions Inhibit Biofilm Formation on Biomaterials
title_full_unstemmed Maggot Excretions Inhibit Biofilm Formation on Biomaterials
title_short Maggot Excretions Inhibit Biofilm Formation on Biomaterials
title_sort maggot excretions inhibit biofilm formation on biomaterials
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2939353/
https://www.ncbi.nlm.nih.gov/pubmed/20309656
http://dx.doi.org/10.1007/s11999-010-1309-5
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