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Rosette nanotubes inhibit bovine neutrophil chemotaxis
Migration of activated neutrophils that have prolonged lifespan into inflamed organs is an important component of host defense but also contributes to tissue damage and mortality. In this report, we used biologically-inspired RGD-tagged rosette nanotubes (RNT) to inhibit neutrophil chemotaxis. We hy...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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EDP Sciences
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2939700/ https://www.ncbi.nlm.nih.gov/pubmed/20663476 http://dx.doi.org/10.1051/vetres/2010047 |
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author | Le, Minh Hong Anh Suri, Sarabjeet Singh Rakotondradany, Felaniaina Fenniri, Hicham Singh, Baljit |
author_facet | Le, Minh Hong Anh Suri, Sarabjeet Singh Rakotondradany, Felaniaina Fenniri, Hicham Singh, Baljit |
author_sort | Le, Minh Hong Anh |
collection | PubMed |
description | Migration of activated neutrophils that have prolonged lifespan into inflamed organs is an important component of host defense but also contributes to tissue damage and mortality. In this report, we used biologically-inspired RGD-tagged rosette nanotubes (RNT) to inhibit neutrophil chemotaxis. We hypothesize that RGD-RNT will block neutrophil migration through inhibition of MAPK. In this report, RNT conjugated to lysine (K–RNT) and arginine-glycine-aspartic acid-serine-lysine (RGDSK-RNT) were co-assembled in a molar ratio of 95/5. The effect of the resulting composite RNT (RGDSK/K–RNT) on neutrophil chemotaxis, cell signaling and apoptosis was then investigated. Exposure to RGDSK/K–RNT reduced bovine neutrophil migration when compared to the non-treated group (p < 0.001). Similar effect was seen following treatment with ERK1/2 or p38 MAPK inhibitors. Phosphorylation of the ERK1/2 and p38 MAPK was inhibited at 5 min by RGDSK/K–RNT (p < 0.05). The RGDSD/K-RNT did not affect the migration of neutrophils pre-treated with αvβ3 integrin antibody suggesting that both bind to the same receptor. RGDSK/K–RNT did not induce apoptosis in bovine neutrophils, which was suppressed by pre-exposing them to LPS (p < 0.001). We conclude that RGDSK/K–RNT inhibit phosphorylation of ERK1/2 and p38 MAPK and inhibit chemotaxis of bovine neutrophils. |
format | Text |
id | pubmed-2939700 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | EDP Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-29397002010-09-20 Rosette nanotubes inhibit bovine neutrophil chemotaxis Le, Minh Hong Anh Suri, Sarabjeet Singh Rakotondradany, Felaniaina Fenniri, Hicham Singh, Baljit Vet Res Original Article Migration of activated neutrophils that have prolonged lifespan into inflamed organs is an important component of host defense but also contributes to tissue damage and mortality. In this report, we used biologically-inspired RGD-tagged rosette nanotubes (RNT) to inhibit neutrophil chemotaxis. We hypothesize that RGD-RNT will block neutrophil migration through inhibition of MAPK. In this report, RNT conjugated to lysine (K–RNT) and arginine-glycine-aspartic acid-serine-lysine (RGDSK-RNT) were co-assembled in a molar ratio of 95/5. The effect of the resulting composite RNT (RGDSK/K–RNT) on neutrophil chemotaxis, cell signaling and apoptosis was then investigated. Exposure to RGDSK/K–RNT reduced bovine neutrophil migration when compared to the non-treated group (p < 0.001). Similar effect was seen following treatment with ERK1/2 or p38 MAPK inhibitors. Phosphorylation of the ERK1/2 and p38 MAPK was inhibited at 5 min by RGDSK/K–RNT (p < 0.05). The RGDSD/K-RNT did not affect the migration of neutrophils pre-treated with αvβ3 integrin antibody suggesting that both bind to the same receptor. RGDSK/K–RNT did not induce apoptosis in bovine neutrophils, which was suppressed by pre-exposing them to LPS (p < 0.001). We conclude that RGDSK/K–RNT inhibit phosphorylation of ERK1/2 and p38 MAPK and inhibit chemotaxis of bovine neutrophils. EDP Sciences 2010-07-29 2010 /pmc/articles/PMC2939700/ /pubmed/20663476 http://dx.doi.org/10.1051/vetres/2010047 Text en © The authors published by INRA/EDP Sciences, 2010 This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted use, distribution, and reproduction in any noncommercial medium, provided the original work is properly cited. |
spellingShingle | Original Article Le, Minh Hong Anh Suri, Sarabjeet Singh Rakotondradany, Felaniaina Fenniri, Hicham Singh, Baljit Rosette nanotubes inhibit bovine neutrophil chemotaxis |
title | Rosette nanotubes inhibit bovine neutrophil chemotaxis |
title_full | Rosette nanotubes inhibit bovine neutrophil chemotaxis |
title_fullStr | Rosette nanotubes inhibit bovine neutrophil chemotaxis |
title_full_unstemmed | Rosette nanotubes inhibit bovine neutrophil chemotaxis |
title_short | Rosette nanotubes inhibit bovine neutrophil chemotaxis |
title_sort | rosette nanotubes inhibit bovine neutrophil chemotaxis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2939700/ https://www.ncbi.nlm.nih.gov/pubmed/20663476 http://dx.doi.org/10.1051/vetres/2010047 |
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