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Generation of Genic Diversity among Streptococcus pneumoniae Strains via Horizontal Gene Transfer during a Chronic Polyclonal Pediatric Infection

Although there is tremendous interest in understanding the evolutionary roles of horizontal gene transfer (HGT) processes that occur during chronic polyclonal infections, to date there have been few studies that directly address this topic. We have characterized multiple HGT events that most likely...

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Autores principales: Hiller, N. Luisa, Ahmed, Azad, Powell, Evan, Martin, Darren P., Eutsey, Rory, Earl, Josh, Janto, Benjamin, Boissy, Robert J., Hogg, Justin, Barbadora, Karen, Sampath, Rangarajan, Lonergan, Shaun, Post, J. Christopher, Hu, Fen Z., Ehrlich, Garth D.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2940740/
https://www.ncbi.nlm.nih.gov/pubmed/20862314
http://dx.doi.org/10.1371/journal.ppat.1001108
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author Hiller, N. Luisa
Ahmed, Azad
Powell, Evan
Martin, Darren P.
Eutsey, Rory
Earl, Josh
Janto, Benjamin
Boissy, Robert J.
Hogg, Justin
Barbadora, Karen
Sampath, Rangarajan
Lonergan, Shaun
Post, J. Christopher
Hu, Fen Z.
Ehrlich, Garth D.
author_facet Hiller, N. Luisa
Ahmed, Azad
Powell, Evan
Martin, Darren P.
Eutsey, Rory
Earl, Josh
Janto, Benjamin
Boissy, Robert J.
Hogg, Justin
Barbadora, Karen
Sampath, Rangarajan
Lonergan, Shaun
Post, J. Christopher
Hu, Fen Z.
Ehrlich, Garth D.
author_sort Hiller, N. Luisa
collection PubMed
description Although there is tremendous interest in understanding the evolutionary roles of horizontal gene transfer (HGT) processes that occur during chronic polyclonal infections, to date there have been few studies that directly address this topic. We have characterized multiple HGT events that most likely occurred during polyclonal infection among nasopharyngeal strains of Streptococcus pneumoniae recovered from a child suffering from chronic upper respiratory and middle-ear infections. Whole genome sequencing and comparative genomics were performed on six isolates collected during symptomatic episodes over a period of seven months. From these comparisons we determined that five of the isolates were genetically highly similar and likely represented a dominant lineage. We analyzed all genic and allelic differences among all six isolates and found that all differences tended to occur within contiguous genomic blocks, suggestive of strain evolution by homologous recombination. From these analyses we identified three strains (two of which were recovered on two different occasions) that appear to have been derived sequentially, one from the next, each by multiple recombination events. We also identified a fourth strain that contains many of the genomic segments that differentiate the three highly related strains from one another, and have hypothesized that this fourth strain may have served as a donor multiple times in the evolution of the dominant strain line. The variations among the parent, daughter, and grand-daughter recombinant strains collectively cover greater than seven percent of the genome and are grouped into 23 chromosomal clusters. While capturing in vivo HGT, these data support the distributed genome hypothesis and suggest that a single competence event in pneumococci can result in the replacement of DNA at multiple non-adjacent loci.
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spelling pubmed-29407402010-09-22 Generation of Genic Diversity among Streptococcus pneumoniae Strains via Horizontal Gene Transfer during a Chronic Polyclonal Pediatric Infection Hiller, N. Luisa Ahmed, Azad Powell, Evan Martin, Darren P. Eutsey, Rory Earl, Josh Janto, Benjamin Boissy, Robert J. Hogg, Justin Barbadora, Karen Sampath, Rangarajan Lonergan, Shaun Post, J. Christopher Hu, Fen Z. Ehrlich, Garth D. PLoS Pathog Research Article Although there is tremendous interest in understanding the evolutionary roles of horizontal gene transfer (HGT) processes that occur during chronic polyclonal infections, to date there have been few studies that directly address this topic. We have characterized multiple HGT events that most likely occurred during polyclonal infection among nasopharyngeal strains of Streptococcus pneumoniae recovered from a child suffering from chronic upper respiratory and middle-ear infections. Whole genome sequencing and comparative genomics were performed on six isolates collected during symptomatic episodes over a period of seven months. From these comparisons we determined that five of the isolates were genetically highly similar and likely represented a dominant lineage. We analyzed all genic and allelic differences among all six isolates and found that all differences tended to occur within contiguous genomic blocks, suggestive of strain evolution by homologous recombination. From these analyses we identified three strains (two of which were recovered on two different occasions) that appear to have been derived sequentially, one from the next, each by multiple recombination events. We also identified a fourth strain that contains many of the genomic segments that differentiate the three highly related strains from one another, and have hypothesized that this fourth strain may have served as a donor multiple times in the evolution of the dominant strain line. The variations among the parent, daughter, and grand-daughter recombinant strains collectively cover greater than seven percent of the genome and are grouped into 23 chromosomal clusters. While capturing in vivo HGT, these data support the distributed genome hypothesis and suggest that a single competence event in pneumococci can result in the replacement of DNA at multiple non-adjacent loci. Public Library of Science 2010-09-16 /pmc/articles/PMC2940740/ /pubmed/20862314 http://dx.doi.org/10.1371/journal.ppat.1001108 Text en Hiller et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Hiller, N. Luisa
Ahmed, Azad
Powell, Evan
Martin, Darren P.
Eutsey, Rory
Earl, Josh
Janto, Benjamin
Boissy, Robert J.
Hogg, Justin
Barbadora, Karen
Sampath, Rangarajan
Lonergan, Shaun
Post, J. Christopher
Hu, Fen Z.
Ehrlich, Garth D.
Generation of Genic Diversity among Streptococcus pneumoniae Strains via Horizontal Gene Transfer during a Chronic Polyclonal Pediatric Infection
title Generation of Genic Diversity among Streptococcus pneumoniae Strains via Horizontal Gene Transfer during a Chronic Polyclonal Pediatric Infection
title_full Generation of Genic Diversity among Streptococcus pneumoniae Strains via Horizontal Gene Transfer during a Chronic Polyclonal Pediatric Infection
title_fullStr Generation of Genic Diversity among Streptococcus pneumoniae Strains via Horizontal Gene Transfer during a Chronic Polyclonal Pediatric Infection
title_full_unstemmed Generation of Genic Diversity among Streptococcus pneumoniae Strains via Horizontal Gene Transfer during a Chronic Polyclonal Pediatric Infection
title_short Generation of Genic Diversity among Streptococcus pneumoniae Strains via Horizontal Gene Transfer during a Chronic Polyclonal Pediatric Infection
title_sort generation of genic diversity among streptococcus pneumoniae strains via horizontal gene transfer during a chronic polyclonal pediatric infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2940740/
https://www.ncbi.nlm.nih.gov/pubmed/20862314
http://dx.doi.org/10.1371/journal.ppat.1001108
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