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Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2

Monocyte subsets have been shown to differ in the pattern of chemokine receptor expression and their migratory properties, both in human and mouse. Previously we have characterized in the swine several monocyte subpopulations, based on the expression of CD163, Tük4 and SLA-II, which share features w...

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Autores principales: Moreno, Sara, Alvarez, Belén, Poderoso, Teresa, Revilla, Concepción, Ezquerra, Angel, Alonso, Fernando, Dominguez, Javier
Formato: Texto
Lenguaje:English
Publicado: EDP Sciences 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2941139/
https://www.ncbi.nlm.nih.gov/pubmed/20670605
http://dx.doi.org/10.1051/vetres/2010048
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author Moreno, Sara
Alvarez, Belén
Poderoso, Teresa
Revilla, Concepción
Ezquerra, Angel
Alonso, Fernando
Dominguez, Javier
author_facet Moreno, Sara
Alvarez, Belén
Poderoso, Teresa
Revilla, Concepción
Ezquerra, Angel
Alonso, Fernando
Dominguez, Javier
author_sort Moreno, Sara
collection PubMed
description Monocyte subsets have been shown to differ in the pattern of chemokine receptor expression and their migratory properties, both in human and mouse. Previously we have characterized in the swine several monocyte subpopulations, based on the expression of CD163, Tük4 and SLA-II, which share features with the populations described in human and mouse. Here, we have analysed the expression of different chemokine receptors in the CD163(−)Tük4(+)SLA-II(−) and CD163(+)Tük4(−)SLA-II(+) populations of porcine monocytes. CD163(+)Tük4(−)SLA-II(+) monocytes expressed higher CX(3)CR1 but lower CCR2 and CXCR4 mRNA levels than CD163(−)Tük4(+)SLA-II(−) monocytes. Moreover, porcine CCL2 binding on Tük4(+)SLA-II(−) but not on Tük4(−)SLA-II(+) cells was detected by using a CCL2-green fluorescence protein (pCCL2-GFP) fusion protein. Finally, flow cytometric analyses of monocytes recovered after chemotaxis assays show a clear increase in the proportion of Tük4(+)SLA-II(−) cells in the fraction migrating toward CCL2, consistent with the polarized CCR2 expression in this monocyte population. The pattern of expression of these chemokine receptors reinforces the similarities of these porcine subsets with their human and mouse counterparts.
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spelling pubmed-29411392010-09-21 Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2 Moreno, Sara Alvarez, Belén Poderoso, Teresa Revilla, Concepción Ezquerra, Angel Alonso, Fernando Dominguez, Javier Vet Res Original Article Monocyte subsets have been shown to differ in the pattern of chemokine receptor expression and their migratory properties, both in human and mouse. Previously we have characterized in the swine several monocyte subpopulations, based on the expression of CD163, Tük4 and SLA-II, which share features with the populations described in human and mouse. Here, we have analysed the expression of different chemokine receptors in the CD163(−)Tük4(+)SLA-II(−) and CD163(+)Tük4(−)SLA-II(+) populations of porcine monocytes. CD163(+)Tük4(−)SLA-II(+) monocytes expressed higher CX(3)CR1 but lower CCR2 and CXCR4 mRNA levels than CD163(−)Tük4(+)SLA-II(−) monocytes. Moreover, porcine CCL2 binding on Tük4(+)SLA-II(−) but not on Tük4(−)SLA-II(+) cells was detected by using a CCL2-green fluorescence protein (pCCL2-GFP) fusion protein. Finally, flow cytometric analyses of monocytes recovered after chemotaxis assays show a clear increase in the proportion of Tük4(+)SLA-II(−) cells in the fraction migrating toward CCL2, consistent with the polarized CCR2 expression in this monocyte population. The pattern of expression of these chemokine receptors reinforces the similarities of these porcine subsets with their human and mouse counterparts. EDP Sciences 2010-07-30 2010 /pmc/articles/PMC2941139/ /pubmed/20670605 http://dx.doi.org/10.1051/vetres/2010048 Text en © The authors published by INRA/EDP Sciences, 2010 This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted use, distribution, and reproduction in any noncommercial medium, provided the original work is properly cited.
spellingShingle Original Article
Moreno, Sara
Alvarez, Belén
Poderoso, Teresa
Revilla, Concepción
Ezquerra, Angel
Alonso, Fernando
Dominguez, Javier
Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2
title Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2
title_full Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2
title_fullStr Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2
title_full_unstemmed Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2
title_short Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2
title_sort porcine monocyte subsets differ in the expression of ccr2 and in their responsiveness to ccl2
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2941139/
https://www.ncbi.nlm.nih.gov/pubmed/20670605
http://dx.doi.org/10.1051/vetres/2010048
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