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Renal effects of Mammea africana Sabine (Guttiferae) stem bark methanol/methylene chloride extract on L-NAME hypertensive rats
OBJECTIVE: The present study aims at evaluating the effects of methanol/methylene chloride extract of the stem bark of Mammea africana on the renal function of L-NAME treated rats. MATERIAL AND METHODS: Normotensive male Wistar rats were divided into five groups respectively treated with distilled w...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Medknow Publications
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2941609/ https://www.ncbi.nlm.nih.gov/pubmed/20927244 http://dx.doi.org/10.4103/0253-7613.68418 |
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author | Nguelefack-Mbuyo, Elvine Pami Dimo, Théophile Nguelefack, Télesphore Benoit Dongmo, Alain Bertrand Kamtchouing, Pierre Kamanyi, Albert |
author_facet | Nguelefack-Mbuyo, Elvine Pami Dimo, Théophile Nguelefack, Télesphore Benoit Dongmo, Alain Bertrand Kamtchouing, Pierre Kamanyi, Albert |
author_sort | Nguelefack-Mbuyo, Elvine Pami |
collection | PubMed |
description | OBJECTIVE: The present study aims at evaluating the effects of methanol/methylene chloride extract of the stem bark of Mammea africana on the renal function of L-NAME treated rats. MATERIAL AND METHODS: Normotensive male Wistar rats were divided into five groups respectively treated with distilled water, L-NAME (40 mg/kg/day), L-NAME + L-arginine (100 mg/kg/day), L-NAME + captopril (20 mg/kg/day) or L-NAME + M. africana extract (200 mg/kg/day) for 30 days. Systolic blood pressure was measured before and at the end of treatment. Body weight was measured at the end of each week. Urine was collected 6 and 24 h after the first administration and further on day 15 and 30 of treatment for creatinine, sodium and potassium quantification, while plasma was collected at the end of treatment for the creatinine assay. ANOVA two way followed by Bonferonni or one way followed by Tukey were used for statistical analysis. RESULTS: M. africana successfully prevented the rise in blood pressure and the acute natriuresis and diuresis induced by L-NAME. When given chronically, the extract produced a sustained antinatriuretic effect, a non-significant increase in urine excretion and reduced the glomerular hyperfiltration induced by L-NAME. CONCLUSIONS: The above results suggest that the methanol/methylene chloride extract of the stem bark of M. africana may protect kidney against renal dysfunction and further demonstrate that its antihypertensive effect does not depend on a diuretic or natriuretic activity. |
format | Text |
id | pubmed-2941609 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Medknow Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-29416092010-10-06 Renal effects of Mammea africana Sabine (Guttiferae) stem bark methanol/methylene chloride extract on L-NAME hypertensive rats Nguelefack-Mbuyo, Elvine Pami Dimo, Théophile Nguelefack, Télesphore Benoit Dongmo, Alain Bertrand Kamtchouing, Pierre Kamanyi, Albert Indian J Pharmacol Research Article OBJECTIVE: The present study aims at evaluating the effects of methanol/methylene chloride extract of the stem bark of Mammea africana on the renal function of L-NAME treated rats. MATERIAL AND METHODS: Normotensive male Wistar rats were divided into five groups respectively treated with distilled water, L-NAME (40 mg/kg/day), L-NAME + L-arginine (100 mg/kg/day), L-NAME + captopril (20 mg/kg/day) or L-NAME + M. africana extract (200 mg/kg/day) for 30 days. Systolic blood pressure was measured before and at the end of treatment. Body weight was measured at the end of each week. Urine was collected 6 and 24 h after the first administration and further on day 15 and 30 of treatment for creatinine, sodium and potassium quantification, while plasma was collected at the end of treatment for the creatinine assay. ANOVA two way followed by Bonferonni or one way followed by Tukey were used for statistical analysis. RESULTS: M. africana successfully prevented the rise in blood pressure and the acute natriuresis and diuresis induced by L-NAME. When given chronically, the extract produced a sustained antinatriuretic effect, a non-significant increase in urine excretion and reduced the glomerular hyperfiltration induced by L-NAME. CONCLUSIONS: The above results suggest that the methanol/methylene chloride extract of the stem bark of M. africana may protect kidney against renal dysfunction and further demonstrate that its antihypertensive effect does not depend on a diuretic or natriuretic activity. Medknow Publications 2010-08 /pmc/articles/PMC2941609/ /pubmed/20927244 http://dx.doi.org/10.4103/0253-7613.68418 Text en © Indian Journal of Pharmacology http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Nguelefack-Mbuyo, Elvine Pami Dimo, Théophile Nguelefack, Télesphore Benoit Dongmo, Alain Bertrand Kamtchouing, Pierre Kamanyi, Albert Renal effects of Mammea africana Sabine (Guttiferae) stem bark methanol/methylene chloride extract on L-NAME hypertensive rats |
title | Renal effects of Mammea africana Sabine (Guttiferae) stem bark methanol/methylene chloride extract on L-NAME hypertensive rats |
title_full | Renal effects of Mammea africana Sabine (Guttiferae) stem bark methanol/methylene chloride extract on L-NAME hypertensive rats |
title_fullStr | Renal effects of Mammea africana Sabine (Guttiferae) stem bark methanol/methylene chloride extract on L-NAME hypertensive rats |
title_full_unstemmed | Renal effects of Mammea africana Sabine (Guttiferae) stem bark methanol/methylene chloride extract on L-NAME hypertensive rats |
title_short | Renal effects of Mammea africana Sabine (Guttiferae) stem bark methanol/methylene chloride extract on L-NAME hypertensive rats |
title_sort | renal effects of mammea africana sabine (guttiferae) stem bark methanol/methylene chloride extract on l-name hypertensive rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2941609/ https://www.ncbi.nlm.nih.gov/pubmed/20927244 http://dx.doi.org/10.4103/0253-7613.68418 |
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