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Evaluation of Cassia occidentalis for in vitro cytotoxicity against human cancer cell lines and antibacterial activity

OBJECTIVE: To evaluate the in vitro cytotoxicity and antibacterial properties of Cassia occidentalis (whole plant) via alcoholic, hydro-alcoholic, and aqueous extracts against eight human cancer cell lines from six different tissues and four bacterial strains. MATERIAL AND METHODS: in vitro cytotoxi...

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Detalles Bibliográficos
Autores principales: Bhagat, Madhulika, Saxena, Ajit Kumar
Formato: Texto
Lenguaje:English
Publicado: Medknow Publications 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2941614/
https://www.ncbi.nlm.nih.gov/pubmed/20927249
http://dx.doi.org/10.4103/0253-7613.68428
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author Bhagat, Madhulika
Saxena, Ajit Kumar
author_facet Bhagat, Madhulika
Saxena, Ajit Kumar
author_sort Bhagat, Madhulika
collection PubMed
description OBJECTIVE: To evaluate the in vitro cytotoxicity and antibacterial properties of Cassia occidentalis (whole plant) via alcoholic, hydro-alcoholic, and aqueous extracts against eight human cancer cell lines from six different tissues and four bacterial strains. MATERIAL AND METHODS: in vitro cytotoxicity against the human cancer cells, cultured for 48h in presence of different concentrations C. occidentalis extracts and percentage of cell viability, was evaluated using the sulforhodamine-B (SRB) assay. The antibacterial activity was performed using the standard protocol against bacterial strains. RESULTS: It was observed that aqueous extract of C. occidentalis (whole plant) had more potential than hydro-alcoholic and alcoholic extracts against HCT-15, SW-620, PC-3, MCF-7, SiHa, and OVCAR-5 human cancer cell lines at 100, 30, and 10 μg/ml in a dose-dependent manner. The hydro-alcoholic extract showed potential against Bacillus subtillis. CONCLUSION: The plant can be explored for the possible development of lead molecules for drug discovery.
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spelling pubmed-29416142010-10-06 Evaluation of Cassia occidentalis for in vitro cytotoxicity against human cancer cell lines and antibacterial activity Bhagat, Madhulika Saxena, Ajit Kumar Indian J Pharmacol Research Article OBJECTIVE: To evaluate the in vitro cytotoxicity and antibacterial properties of Cassia occidentalis (whole plant) via alcoholic, hydro-alcoholic, and aqueous extracts against eight human cancer cell lines from six different tissues and four bacterial strains. MATERIAL AND METHODS: in vitro cytotoxicity against the human cancer cells, cultured for 48h in presence of different concentrations C. occidentalis extracts and percentage of cell viability, was evaluated using the sulforhodamine-B (SRB) assay. The antibacterial activity was performed using the standard protocol against bacterial strains. RESULTS: It was observed that aqueous extract of C. occidentalis (whole plant) had more potential than hydro-alcoholic and alcoholic extracts against HCT-15, SW-620, PC-3, MCF-7, SiHa, and OVCAR-5 human cancer cell lines at 100, 30, and 10 μg/ml in a dose-dependent manner. The hydro-alcoholic extract showed potential against Bacillus subtillis. CONCLUSION: The plant can be explored for the possible development of lead molecules for drug discovery. Medknow Publications 2010-08 /pmc/articles/PMC2941614/ /pubmed/20927249 http://dx.doi.org/10.4103/0253-7613.68428 Text en © Indian Journal of Pharmacology http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bhagat, Madhulika
Saxena, Ajit Kumar
Evaluation of Cassia occidentalis for in vitro cytotoxicity against human cancer cell lines and antibacterial activity
title Evaluation of Cassia occidentalis for in vitro cytotoxicity against human cancer cell lines and antibacterial activity
title_full Evaluation of Cassia occidentalis for in vitro cytotoxicity against human cancer cell lines and antibacterial activity
title_fullStr Evaluation of Cassia occidentalis for in vitro cytotoxicity against human cancer cell lines and antibacterial activity
title_full_unstemmed Evaluation of Cassia occidentalis for in vitro cytotoxicity against human cancer cell lines and antibacterial activity
title_short Evaluation of Cassia occidentalis for in vitro cytotoxicity against human cancer cell lines and antibacterial activity
title_sort evaluation of cassia occidentalis for in vitro cytotoxicity against human cancer cell lines and antibacterial activity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2941614/
https://www.ncbi.nlm.nih.gov/pubmed/20927249
http://dx.doi.org/10.4103/0253-7613.68428
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