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Evaluation of Cassia occidentalis for in vitro cytotoxicity against human cancer cell lines and antibacterial activity
OBJECTIVE: To evaluate the in vitro cytotoxicity and antibacterial properties of Cassia occidentalis (whole plant) via alcoholic, hydro-alcoholic, and aqueous extracts against eight human cancer cell lines from six different tissues and four bacterial strains. MATERIAL AND METHODS: in vitro cytotoxi...
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Formato: | Texto |
Lenguaje: | English |
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Medknow Publications
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2941614/ https://www.ncbi.nlm.nih.gov/pubmed/20927249 http://dx.doi.org/10.4103/0253-7613.68428 |
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author | Bhagat, Madhulika Saxena, Ajit Kumar |
author_facet | Bhagat, Madhulika Saxena, Ajit Kumar |
author_sort | Bhagat, Madhulika |
collection | PubMed |
description | OBJECTIVE: To evaluate the in vitro cytotoxicity and antibacterial properties of Cassia occidentalis (whole plant) via alcoholic, hydro-alcoholic, and aqueous extracts against eight human cancer cell lines from six different tissues and four bacterial strains. MATERIAL AND METHODS: in vitro cytotoxicity against the human cancer cells, cultured for 48h in presence of different concentrations C. occidentalis extracts and percentage of cell viability, was evaluated using the sulforhodamine-B (SRB) assay. The antibacterial activity was performed using the standard protocol against bacterial strains. RESULTS: It was observed that aqueous extract of C. occidentalis (whole plant) had more potential than hydro-alcoholic and alcoholic extracts against HCT-15, SW-620, PC-3, MCF-7, SiHa, and OVCAR-5 human cancer cell lines at 100, 30, and 10 μg/ml in a dose-dependent manner. The hydro-alcoholic extract showed potential against Bacillus subtillis. CONCLUSION: The plant can be explored for the possible development of lead molecules for drug discovery. |
format | Text |
id | pubmed-2941614 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Medknow Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-29416142010-10-06 Evaluation of Cassia occidentalis for in vitro cytotoxicity against human cancer cell lines and antibacterial activity Bhagat, Madhulika Saxena, Ajit Kumar Indian J Pharmacol Research Article OBJECTIVE: To evaluate the in vitro cytotoxicity and antibacterial properties of Cassia occidentalis (whole plant) via alcoholic, hydro-alcoholic, and aqueous extracts against eight human cancer cell lines from six different tissues and four bacterial strains. MATERIAL AND METHODS: in vitro cytotoxicity against the human cancer cells, cultured for 48h in presence of different concentrations C. occidentalis extracts and percentage of cell viability, was evaluated using the sulforhodamine-B (SRB) assay. The antibacterial activity was performed using the standard protocol against bacterial strains. RESULTS: It was observed that aqueous extract of C. occidentalis (whole plant) had more potential than hydro-alcoholic and alcoholic extracts against HCT-15, SW-620, PC-3, MCF-7, SiHa, and OVCAR-5 human cancer cell lines at 100, 30, and 10 μg/ml in a dose-dependent manner. The hydro-alcoholic extract showed potential against Bacillus subtillis. CONCLUSION: The plant can be explored for the possible development of lead molecules for drug discovery. Medknow Publications 2010-08 /pmc/articles/PMC2941614/ /pubmed/20927249 http://dx.doi.org/10.4103/0253-7613.68428 Text en © Indian Journal of Pharmacology http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Bhagat, Madhulika Saxena, Ajit Kumar Evaluation of Cassia occidentalis for in vitro cytotoxicity against human cancer cell lines and antibacterial activity |
title | Evaluation of Cassia occidentalis for in vitro cytotoxicity against human cancer cell lines and antibacterial activity |
title_full | Evaluation of Cassia occidentalis for in vitro cytotoxicity against human cancer cell lines and antibacterial activity |
title_fullStr | Evaluation of Cassia occidentalis for in vitro cytotoxicity against human cancer cell lines and antibacterial activity |
title_full_unstemmed | Evaluation of Cassia occidentalis for in vitro cytotoxicity against human cancer cell lines and antibacterial activity |
title_short | Evaluation of Cassia occidentalis for in vitro cytotoxicity against human cancer cell lines and antibacterial activity |
title_sort | evaluation of cassia occidentalis for in vitro cytotoxicity against human cancer cell lines and antibacterial activity |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2941614/ https://www.ncbi.nlm.nih.gov/pubmed/20927249 http://dx.doi.org/10.4103/0253-7613.68428 |
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