High-throughput in vivo vertebrate screening

We demonstrate a high-throughput platform for cellular-resolution in vivo pharmaceutical and genetic screens on zebrafish larvae. The system automatically loads animals from reservoirs or multiwell plates, and positions and orients them for high-speed confocal imaging and laser manipulation of both...

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Detalles Bibliográficos
Autores principales: Pardo-Martin, Carlos, Chang, Tsung-Yao, Koo, Bryan Kyo, Gilleland, Cody L., Wasserman, Steven C., Yanik, Mehmet Fatih
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2941625/
https://www.ncbi.nlm.nih.gov/pubmed/20639868
http://dx.doi.org/10.1038/nmeth.1481
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author Pardo-Martin, Carlos
Chang, Tsung-Yao
Koo, Bryan Kyo
Gilleland, Cody L.
Wasserman, Steven C.
Yanik, Mehmet Fatih
author_facet Pardo-Martin, Carlos
Chang, Tsung-Yao
Koo, Bryan Kyo
Gilleland, Cody L.
Wasserman, Steven C.
Yanik, Mehmet Fatih
author_sort Pardo-Martin, Carlos
collection PubMed
description We demonstrate a high-throughput platform for cellular-resolution in vivo pharmaceutical and genetic screens on zebrafish larvae. The system automatically loads animals from reservoirs or multiwell plates, and positions and orients them for high-speed confocal imaging and laser manipulation of both superficial and deep organs within 19 seconds without damage. We show small-scale test screening of retinal axon guidance mutants and neuronal regeneration assays in combination with femtosecond laser microsurgery.
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spelling pubmed-29416252011-02-01 High-throughput in vivo vertebrate screening Pardo-Martin, Carlos Chang, Tsung-Yao Koo, Bryan Kyo Gilleland, Cody L. Wasserman, Steven C. Yanik, Mehmet Fatih Nat Methods Article We demonstrate a high-throughput platform for cellular-resolution in vivo pharmaceutical and genetic screens on zebrafish larvae. The system automatically loads animals from reservoirs or multiwell plates, and positions and orients them for high-speed confocal imaging and laser manipulation of both superficial and deep organs within 19 seconds without damage. We show small-scale test screening of retinal axon guidance mutants and neuronal regeneration assays in combination with femtosecond laser microsurgery. 2010-07-18 2010-08 /pmc/articles/PMC2941625/ /pubmed/20639868 http://dx.doi.org/10.1038/nmeth.1481 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Pardo-Martin, Carlos
Chang, Tsung-Yao
Koo, Bryan Kyo
Gilleland, Cody L.
Wasserman, Steven C.
Yanik, Mehmet Fatih
High-throughput in vivo vertebrate screening
title High-throughput in vivo vertebrate screening
title_full High-throughput in vivo vertebrate screening
title_fullStr High-throughput in vivo vertebrate screening
title_full_unstemmed High-throughput in vivo vertebrate screening
title_short High-throughput in vivo vertebrate screening
title_sort high-throughput in vivo vertebrate screening
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2941625/
https://www.ncbi.nlm.nih.gov/pubmed/20639868
http://dx.doi.org/10.1038/nmeth.1481
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