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Quantification of Persistence of Escherichia coli O157:H7 in Contrasting Soils

Persistence of Escherichia coli (E. coli) O157:H7 in the environment is a major concern to vegetable and fruit growers where farms and livestock production are in close proximity. The objectives were to determine the effects of preplant fumigation treatment on the survival of E. coli O157:H7 in two...

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Autores principales: Ibekwe, A. Mark, Papiernik, Sharon K., Grieve, Catherine M., Yang, Ching-Hong
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2943103/
https://www.ncbi.nlm.nih.gov/pubmed/20871863
http://dx.doi.org/10.1155/2011/421379
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author Ibekwe, A. Mark
Papiernik, Sharon K.
Grieve, Catherine M.
Yang, Ching-Hong
author_facet Ibekwe, A. Mark
Papiernik, Sharon K.
Grieve, Catherine M.
Yang, Ching-Hong
author_sort Ibekwe, A. Mark
collection PubMed
description Persistence of Escherichia coli (E. coli) O157:H7 in the environment is a major concern to vegetable and fruit growers where farms and livestock production are in close proximity. The objectives were to determine the effects of preplant fumigation treatment on the survival of E. coli O157:H7 in two soils and the effects of indigenous bacterial populations on the survival of this pathogen. Real-time PCR and plate counts were used to quantify the survival of E. coli O157:H7 in two contrasting soils after fumigation with methyl bromide (MeBr) and methyl iodide (MeI). Ten days after fumigation, E. coli O157:H7 counts were significantly lower (P = .0001) in fumigated soils than in the non-fumigated. Direct comparison between MeBr and MeI within each soil indicated that these two fumigants showed similar impacts on E. coli O157:H7 survival. Microbial species diversity as determined by DGGE was significantly higher in clay soil than sandy soil and this resulted in higher initial decline in population in clay soil than in sandy soil. This study shows that if soil is contaminated with E. coli O157:H7, fumigation alone may not eliminate the pathogen, but may cause decrease in microbial diversity which may enhance the survival of the pathogen.
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spelling pubmed-29431032010-09-24 Quantification of Persistence of Escherichia coli O157:H7 in Contrasting Soils Ibekwe, A. Mark Papiernik, Sharon K. Grieve, Catherine M. Yang, Ching-Hong Int J Microbiol Research Article Persistence of Escherichia coli (E. coli) O157:H7 in the environment is a major concern to vegetable and fruit growers where farms and livestock production are in close proximity. The objectives were to determine the effects of preplant fumigation treatment on the survival of E. coli O157:H7 in two soils and the effects of indigenous bacterial populations on the survival of this pathogen. Real-time PCR and plate counts were used to quantify the survival of E. coli O157:H7 in two contrasting soils after fumigation with methyl bromide (MeBr) and methyl iodide (MeI). Ten days after fumigation, E. coli O157:H7 counts were significantly lower (P = .0001) in fumigated soils than in the non-fumigated. Direct comparison between MeBr and MeI within each soil indicated that these two fumigants showed similar impacts on E. coli O157:H7 survival. Microbial species diversity as determined by DGGE was significantly higher in clay soil than sandy soil and this resulted in higher initial decline in population in clay soil than in sandy soil. This study shows that if soil is contaminated with E. coli O157:H7, fumigation alone may not eliminate the pathogen, but may cause decrease in microbial diversity which may enhance the survival of the pathogen. Hindawi Publishing Corporation 2011 2010-09-08 /pmc/articles/PMC2943103/ /pubmed/20871863 http://dx.doi.org/10.1155/2011/421379 Text en Copyright © 2011 A. Mark Ibekwe et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ibekwe, A. Mark
Papiernik, Sharon K.
Grieve, Catherine M.
Yang, Ching-Hong
Quantification of Persistence of Escherichia coli O157:H7 in Contrasting Soils
title Quantification of Persistence of Escherichia coli O157:H7 in Contrasting Soils
title_full Quantification of Persistence of Escherichia coli O157:H7 in Contrasting Soils
title_fullStr Quantification of Persistence of Escherichia coli O157:H7 in Contrasting Soils
title_full_unstemmed Quantification of Persistence of Escherichia coli O157:H7 in Contrasting Soils
title_short Quantification of Persistence of Escherichia coli O157:H7 in Contrasting Soils
title_sort quantification of persistence of escherichia coli o157:h7 in contrasting soils
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2943103/
https://www.ncbi.nlm.nih.gov/pubmed/20871863
http://dx.doi.org/10.1155/2011/421379
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