Cargando…
PPARG: Gene Expression Regulation and Next-Generation Sequencing for Unsolved Issues
Peroxisome proliferator-activated receptor gamma (PPARγ) is one of the most extensively studied ligand-inducible transcription factors (TFs), able to modulate its transcriptional activity through conformational changes. It is of particular interest because of its pleiotropic functions: it plays a cr...
Autores principales: | , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2943117/ https://www.ncbi.nlm.nih.gov/pubmed/20871817 http://dx.doi.org/10.1155/2010/409168 |
_version_ | 1782186995622084608 |
---|---|
author | Costa, Valerio Gallo, Maria Assunta Letizia, Francesca Aprile, Marianna Casamassimi, Amelia Ciccodicola, Alfredo |
author_facet | Costa, Valerio Gallo, Maria Assunta Letizia, Francesca Aprile, Marianna Casamassimi, Amelia Ciccodicola, Alfredo |
author_sort | Costa, Valerio |
collection | PubMed |
description | Peroxisome proliferator-activated receptor gamma (PPARγ) is one of the most extensively studied ligand-inducible transcription factors (TFs), able to modulate its transcriptional activity through conformational changes. It is of particular interest because of its pleiotropic functions: it plays a crucial role in the expression of key genes involved in adipogenesis, lipid and glucid metabolism, atherosclerosis, inflammation, and cancer. Its protein isoforms, the wide number of PPARγ target genes, ligands, and coregulators contribute to determine the complexity of its function. In addition, the presence of genetic variants is likely to affect expression levels of target genes although the impact of PPARG gene variations on the expression of target genes is not fully understood. The introduction of massively parallel sequencing platforms—in the Next Generation Sequencing (NGS) era—has revolutionized the way of investigating the genetic causes of inherited diseases. In this context, DNA-Seq for identifying—within both coding and regulatory regions of PPARG gene—novel nucleotide variations and haplotypes associated to human diseases, ChIP-Seq for defining a PPARγ binding map, and RNA-Seq for unraveling the wide and intricate gene pathways regulated by PPARG, represent incredible steps toward the understanding of PPARγ in health and disease. |
format | Text |
id | pubmed-2943117 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-29431172010-09-24 PPARG: Gene Expression Regulation and Next-Generation Sequencing for Unsolved Issues Costa, Valerio Gallo, Maria Assunta Letizia, Francesca Aprile, Marianna Casamassimi, Amelia Ciccodicola, Alfredo PPAR Res Review Article Peroxisome proliferator-activated receptor gamma (PPARγ) is one of the most extensively studied ligand-inducible transcription factors (TFs), able to modulate its transcriptional activity through conformational changes. It is of particular interest because of its pleiotropic functions: it plays a crucial role in the expression of key genes involved in adipogenesis, lipid and glucid metabolism, atherosclerosis, inflammation, and cancer. Its protein isoforms, the wide number of PPARγ target genes, ligands, and coregulators contribute to determine the complexity of its function. In addition, the presence of genetic variants is likely to affect expression levels of target genes although the impact of PPARG gene variations on the expression of target genes is not fully understood. The introduction of massively parallel sequencing platforms—in the Next Generation Sequencing (NGS) era—has revolutionized the way of investigating the genetic causes of inherited diseases. In this context, DNA-Seq for identifying—within both coding and regulatory regions of PPARG gene—novel nucleotide variations and haplotypes associated to human diseases, ChIP-Seq for defining a PPARγ binding map, and RNA-Seq for unraveling the wide and intricate gene pathways regulated by PPARG, represent incredible steps toward the understanding of PPARγ in health and disease. Hindawi Publishing Corporation 2010 2010-09-08 /pmc/articles/PMC2943117/ /pubmed/20871817 http://dx.doi.org/10.1155/2010/409168 Text en Copyright © 2010 Valerio Costa et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Article Costa, Valerio Gallo, Maria Assunta Letizia, Francesca Aprile, Marianna Casamassimi, Amelia Ciccodicola, Alfredo PPARG: Gene Expression Regulation and Next-Generation Sequencing for Unsolved Issues |
title | PPARG: Gene Expression Regulation and Next-Generation Sequencing for Unsolved Issues |
title_full | PPARG: Gene Expression Regulation and Next-Generation Sequencing for Unsolved Issues |
title_fullStr | PPARG: Gene Expression Regulation and Next-Generation Sequencing for Unsolved Issues |
title_full_unstemmed | PPARG: Gene Expression Regulation and Next-Generation Sequencing for Unsolved Issues |
title_short | PPARG: Gene Expression Regulation and Next-Generation Sequencing for Unsolved Issues |
title_sort | pparg: gene expression regulation and next-generation sequencing for unsolved issues |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2943117/ https://www.ncbi.nlm.nih.gov/pubmed/20871817 http://dx.doi.org/10.1155/2010/409168 |
work_keys_str_mv | AT costavalerio pparggeneexpressionregulationandnextgenerationsequencingforunsolvedissues AT gallomariaassunta pparggeneexpressionregulationandnextgenerationsequencingforunsolvedissues AT letiziafrancesca pparggeneexpressionregulationandnextgenerationsequencingforunsolvedissues AT aprilemarianna pparggeneexpressionregulationandnextgenerationsequencingforunsolvedissues AT casamassimiamelia pparggeneexpressionregulationandnextgenerationsequencingforunsolvedissues AT ciccodicolaalfredo pparggeneexpressionregulationandnextgenerationsequencingforunsolvedissues |