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Mtmr8 is essential for vasculature development in zebrafish embryos

BACKGROUND: Embryonic morphogenesis of vascular and muscular systems is tightly coordinated, and a functional cooperation of Mtmr8 with PI3K in actin filament modeling and muscle development has been revealed in zebrafish. Here, we attempt to explore the function of Mtmr8 in vasculature development...

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Detalles Bibliográficos
Autores principales: Mei, Jie, Liu, Sha, Li, Zhi, Gui, Jian-Fang
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2944161/
https://www.ncbi.nlm.nih.gov/pubmed/20815916
http://dx.doi.org/10.1186/1471-213X-10-96
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author Mei, Jie
Liu, Sha
Li, Zhi
Gui, Jian-Fang
author_facet Mei, Jie
Liu, Sha
Li, Zhi
Gui, Jian-Fang
author_sort Mei, Jie
collection PubMed
description BACKGROUND: Embryonic morphogenesis of vascular and muscular systems is tightly coordinated, and a functional cooperation of Mtmr8 with PI3K in actin filament modeling and muscle development has been revealed in zebrafish. Here, we attempt to explore the function of Mtmr8 in vasculature development parallel to its function in muscle development. RESULTS: During early stage of somitogenesis, mtmr8 expression was detected in both somitic mesodem and ventral mesoderm. Knockdown of mtmr8 by morpholino impairs arterial endothelial marker expression, and results in endothelial cell reduction and vasculogenesis defects, such as retardation in intersegmental vessel development and interruption of trunk dorsal aorta. Moreover, mtmr8 morphants show loss of arterial endothelial cell identity in dorsal aorta, which is effectively rescued by low concentration of PI3K inhibitor, and by over-expression of dnPKA mRNA or vegf mRNA. Interestingly, mtmr8 expression is up-regulated when zebrafish embryos are treated with specific inhibitor of Hedgehog pathway that abolishes arterial marker expression. CONCLUSION: These data indicate that Mtmr8 is essential for vasculature development in zebrafish embryos, and may play a role in arterial specification through repressing PI3K activity. It is suggested that Mtmr8 should represent a novel element of the Hedgehog/PI3K/VEGF signaling cascade that controls arterial specification.
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spelling pubmed-29441612010-09-24 Mtmr8 is essential for vasculature development in zebrafish embryos Mei, Jie Liu, Sha Li, Zhi Gui, Jian-Fang BMC Dev Biol Research Article BACKGROUND: Embryonic morphogenesis of vascular and muscular systems is tightly coordinated, and a functional cooperation of Mtmr8 with PI3K in actin filament modeling and muscle development has been revealed in zebrafish. Here, we attempt to explore the function of Mtmr8 in vasculature development parallel to its function in muscle development. RESULTS: During early stage of somitogenesis, mtmr8 expression was detected in both somitic mesodem and ventral mesoderm. Knockdown of mtmr8 by morpholino impairs arterial endothelial marker expression, and results in endothelial cell reduction and vasculogenesis defects, such as retardation in intersegmental vessel development and interruption of trunk dorsal aorta. Moreover, mtmr8 morphants show loss of arterial endothelial cell identity in dorsal aorta, which is effectively rescued by low concentration of PI3K inhibitor, and by over-expression of dnPKA mRNA or vegf mRNA. Interestingly, mtmr8 expression is up-regulated when zebrafish embryos are treated with specific inhibitor of Hedgehog pathway that abolishes arterial marker expression. CONCLUSION: These data indicate that Mtmr8 is essential for vasculature development in zebrafish embryos, and may play a role in arterial specification through repressing PI3K activity. It is suggested that Mtmr8 should represent a novel element of the Hedgehog/PI3K/VEGF signaling cascade that controls arterial specification. BioMed Central 2010-09-05 /pmc/articles/PMC2944161/ /pubmed/20815916 http://dx.doi.org/10.1186/1471-213X-10-96 Text en Copyright ©2010 Mei et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mei, Jie
Liu, Sha
Li, Zhi
Gui, Jian-Fang
Mtmr8 is essential for vasculature development in zebrafish embryos
title Mtmr8 is essential for vasculature development in zebrafish embryos
title_full Mtmr8 is essential for vasculature development in zebrafish embryos
title_fullStr Mtmr8 is essential for vasculature development in zebrafish embryos
title_full_unstemmed Mtmr8 is essential for vasculature development in zebrafish embryos
title_short Mtmr8 is essential for vasculature development in zebrafish embryos
title_sort mtmr8 is essential for vasculature development in zebrafish embryos
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2944161/
https://www.ncbi.nlm.nih.gov/pubmed/20815916
http://dx.doi.org/10.1186/1471-213X-10-96
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