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Transcriptional Regulation of an Axonemal Central Apparatus Gene, Sperm-associated Antigen 6, by a SRY-related High Mobility Group Transcription Factor, S-SOX5

SOX5 is a transcription factor with homology to the high mobility group box region of the testis-determining factor, SRY. Both the mouse and human SOX5 genes encode a 48-kDa SOX5 protein (S-SOX5) that is only present in tissues containing cells with motile cilia/flagella. The mammalian sperm-associa...

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Autores principales: Kiselak, Elizabeth Anne, Shen, Xuening, Song, Jingmei, Gude, David Roberto, Wang, Jiannan, Brody, Steven L., Strauss, Jerome F., Zhang, Zhibing
Formato: Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2945543/
https://www.ncbi.nlm.nih.gov/pubmed/20668334
http://dx.doi.org/10.1074/jbc.M110.121590
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author Kiselak, Elizabeth Anne
Shen, Xuening
Song, Jingmei
Gude, David Roberto
Wang, Jiannan
Brody, Steven L.
Strauss, Jerome F.
Zhang, Zhibing
author_facet Kiselak, Elizabeth Anne
Shen, Xuening
Song, Jingmei
Gude, David Roberto
Wang, Jiannan
Brody, Steven L.
Strauss, Jerome F.
Zhang, Zhibing
author_sort Kiselak, Elizabeth Anne
collection PubMed
description SOX5 is a transcription factor with homology to the high mobility group box region of the testis-determining factor, SRY. Both the mouse and human SOX5 genes encode a 48-kDa SOX5 protein (S-SOX5) that is only present in tissues containing cells with motile cilia/flagella. The mammalian sperm-associated antigen 6 gene (SPAG6) encodes an axoneme central apparatus protein. Because human and mouse SPAG6 gene promoters contain multiple potential binding sites for SOX5, SPAG6 gene regulation by S-SOX5 was investigated in BEAS-2B cells, a line derived from human bronchial cells. Like FOXJ1, a transcription factor known to be essential for motile ciliogenesis, S-SOX5 stimulated mouse and human SPAG6 promoter function in BEAS-2B cells, but the effect was abrogated when the SOX5 binding sites were mutated or deleted. S-SOX5 and FOXJ1 functioned cooperatively in stimulating SPAG6 promoter activity. The SPAG6 message was up-regulated when S-SOX5 was overexpressed in BEAS-2B cells, and silencing of S-SOX5 by RNA interference down-regulated SPAG6 transcripts. Chromatin immunoprecipitation and EMSA experiments demonstrated that S-SOX5 associates with the SPAG6 promoter directly. The present study demonstrates that SPAG6 is a S-SOX5 target gene, indicating a key role for S-SOX5 in the formation and function of motile cilia.
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spelling pubmed-29455432010-10-04 Transcriptional Regulation of an Axonemal Central Apparatus Gene, Sperm-associated Antigen 6, by a SRY-related High Mobility Group Transcription Factor, S-SOX5 Kiselak, Elizabeth Anne Shen, Xuening Song, Jingmei Gude, David Roberto Wang, Jiannan Brody, Steven L. Strauss, Jerome F. Zhang, Zhibing J Biol Chem Gene Regulation SOX5 is a transcription factor with homology to the high mobility group box region of the testis-determining factor, SRY. Both the mouse and human SOX5 genes encode a 48-kDa SOX5 protein (S-SOX5) that is only present in tissues containing cells with motile cilia/flagella. The mammalian sperm-associated antigen 6 gene (SPAG6) encodes an axoneme central apparatus protein. Because human and mouse SPAG6 gene promoters contain multiple potential binding sites for SOX5, SPAG6 gene regulation by S-SOX5 was investigated in BEAS-2B cells, a line derived from human bronchial cells. Like FOXJ1, a transcription factor known to be essential for motile ciliogenesis, S-SOX5 stimulated mouse and human SPAG6 promoter function in BEAS-2B cells, but the effect was abrogated when the SOX5 binding sites were mutated or deleted. S-SOX5 and FOXJ1 functioned cooperatively in stimulating SPAG6 promoter activity. The SPAG6 message was up-regulated when S-SOX5 was overexpressed in BEAS-2B cells, and silencing of S-SOX5 by RNA interference down-regulated SPAG6 transcripts. Chromatin immunoprecipitation and EMSA experiments demonstrated that S-SOX5 associates with the SPAG6 promoter directly. The present study demonstrates that SPAG6 is a S-SOX5 target gene, indicating a key role for S-SOX5 in the formation and function of motile cilia. American Society for Biochemistry and Molecular Biology 2010-10-01 2010-07-28 /pmc/articles/PMC2945543/ /pubmed/20668334 http://dx.doi.org/10.1074/jbc.M110.121590 Text en © 2010 by The American Society for Biochemistry and Molecular Biology, Inc. Author's Choice—Final version full access. Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) applies to Author Choice Articles
spellingShingle Gene Regulation
Kiselak, Elizabeth Anne
Shen, Xuening
Song, Jingmei
Gude, David Roberto
Wang, Jiannan
Brody, Steven L.
Strauss, Jerome F.
Zhang, Zhibing
Transcriptional Regulation of an Axonemal Central Apparatus Gene, Sperm-associated Antigen 6, by a SRY-related High Mobility Group Transcription Factor, S-SOX5
title Transcriptional Regulation of an Axonemal Central Apparatus Gene, Sperm-associated Antigen 6, by a SRY-related High Mobility Group Transcription Factor, S-SOX5
title_full Transcriptional Regulation of an Axonemal Central Apparatus Gene, Sperm-associated Antigen 6, by a SRY-related High Mobility Group Transcription Factor, S-SOX5
title_fullStr Transcriptional Regulation of an Axonemal Central Apparatus Gene, Sperm-associated Antigen 6, by a SRY-related High Mobility Group Transcription Factor, S-SOX5
title_full_unstemmed Transcriptional Regulation of an Axonemal Central Apparatus Gene, Sperm-associated Antigen 6, by a SRY-related High Mobility Group Transcription Factor, S-SOX5
title_short Transcriptional Regulation of an Axonemal Central Apparatus Gene, Sperm-associated Antigen 6, by a SRY-related High Mobility Group Transcription Factor, S-SOX5
title_sort transcriptional regulation of an axonemal central apparatus gene, sperm-associated antigen 6, by a sry-related high mobility group transcription factor, s-sox5
topic Gene Regulation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2945543/
https://www.ncbi.nlm.nih.gov/pubmed/20668334
http://dx.doi.org/10.1074/jbc.M110.121590
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