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Comprehensive transcriptome analysis of mouse embryonic stem cell adipogenesis unravels new processes of adipocyte development

BACKGROUND: The current epidemic of obesity has caused a surge of interest in the study of adipose tissue formation. While major progress has been made in defining the molecular networks that control adipocyte terminal differentiation, the early steps of adipocyte development and the embryonic origi...

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Autores principales: Billon, Nathalie, Kolde, Raivo, Reimand, Jüri, Monteiro, Miguel C, Kull, Meelis, Peterson, Hedi, Tretyakov, Konstantin, Adler, Priit, Wdziekonski, Brigitte, Vilo, Jaak, Dani, Christian
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2945782/
https://www.ncbi.nlm.nih.gov/pubmed/20678241
http://dx.doi.org/10.1186/gb-2010-11-8-r80
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author Billon, Nathalie
Kolde, Raivo
Reimand, Jüri
Monteiro, Miguel C
Kull, Meelis
Peterson, Hedi
Tretyakov, Konstantin
Adler, Priit
Wdziekonski, Brigitte
Vilo, Jaak
Dani, Christian
author_facet Billon, Nathalie
Kolde, Raivo
Reimand, Jüri
Monteiro, Miguel C
Kull, Meelis
Peterson, Hedi
Tretyakov, Konstantin
Adler, Priit
Wdziekonski, Brigitte
Vilo, Jaak
Dani, Christian
author_sort Billon, Nathalie
collection PubMed
description BACKGROUND: The current epidemic of obesity has caused a surge of interest in the study of adipose tissue formation. While major progress has been made in defining the molecular networks that control adipocyte terminal differentiation, the early steps of adipocyte development and the embryonic origin of this lineage remain largely unknown. RESULTS: Here we performed genome-wide analysis of gene expression during adipogenesis of mouse embryonic stem cells (ESCs). We then pursued comprehensive bioinformatic analyses, including de novo functional annotation and curation of the generated data within the context of biological pathways, to uncover novel biological functions associated with the early steps of adipocyte development. By combining in-depth gene regulation studies and in silico analysis of transcription factor binding site enrichment, we also provide insights into the transcriptional networks that might govern these early steps. CONCLUSIONS: This study supports several biological findings: firstly, adipocyte development in mouse ESCs is coupled to blood vessel morphogenesis and neural development, just as it is during mouse development. Secondly, the early steps of adipocyte formation involve major changes in signaling and transcriptional networks. A large proportion of the transcription factors that we uncovered in mouse ESCs are also expressed in the mouse embryonic mesenchyme and in adipose tissues, demonstrating the power of our approach to probe for genes associated with early developmental processes on a genome-wide scale. Finally, we reveal a plethora of novel candidate genes for adipocyte development and present a unique resource that can be further explored in functional assays.
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spelling pubmed-29457822010-09-28 Comprehensive transcriptome analysis of mouse embryonic stem cell adipogenesis unravels new processes of adipocyte development Billon, Nathalie Kolde, Raivo Reimand, Jüri Monteiro, Miguel C Kull, Meelis Peterson, Hedi Tretyakov, Konstantin Adler, Priit Wdziekonski, Brigitte Vilo, Jaak Dani, Christian Genome Biol Research BACKGROUND: The current epidemic of obesity has caused a surge of interest in the study of adipose tissue formation. While major progress has been made in defining the molecular networks that control adipocyte terminal differentiation, the early steps of adipocyte development and the embryonic origin of this lineage remain largely unknown. RESULTS: Here we performed genome-wide analysis of gene expression during adipogenesis of mouse embryonic stem cells (ESCs). We then pursued comprehensive bioinformatic analyses, including de novo functional annotation and curation of the generated data within the context of biological pathways, to uncover novel biological functions associated with the early steps of adipocyte development. By combining in-depth gene regulation studies and in silico analysis of transcription factor binding site enrichment, we also provide insights into the transcriptional networks that might govern these early steps. CONCLUSIONS: This study supports several biological findings: firstly, adipocyte development in mouse ESCs is coupled to blood vessel morphogenesis and neural development, just as it is during mouse development. Secondly, the early steps of adipocyte formation involve major changes in signaling and transcriptional networks. A large proportion of the transcription factors that we uncovered in mouse ESCs are also expressed in the mouse embryonic mesenchyme and in adipose tissues, demonstrating the power of our approach to probe for genes associated with early developmental processes on a genome-wide scale. Finally, we reveal a plethora of novel candidate genes for adipocyte development and present a unique resource that can be further explored in functional assays. BioMed Central 2010 2010-08-03 /pmc/articles/PMC2945782/ /pubmed/20678241 http://dx.doi.org/10.1186/gb-2010-11-8-r80 Text en Copyright ©2010 Billon et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Billon, Nathalie
Kolde, Raivo
Reimand, Jüri
Monteiro, Miguel C
Kull, Meelis
Peterson, Hedi
Tretyakov, Konstantin
Adler, Priit
Wdziekonski, Brigitte
Vilo, Jaak
Dani, Christian
Comprehensive transcriptome analysis of mouse embryonic stem cell adipogenesis unravels new processes of adipocyte development
title Comprehensive transcriptome analysis of mouse embryonic stem cell adipogenesis unravels new processes of adipocyte development
title_full Comprehensive transcriptome analysis of mouse embryonic stem cell adipogenesis unravels new processes of adipocyte development
title_fullStr Comprehensive transcriptome analysis of mouse embryonic stem cell adipogenesis unravels new processes of adipocyte development
title_full_unstemmed Comprehensive transcriptome analysis of mouse embryonic stem cell adipogenesis unravels new processes of adipocyte development
title_short Comprehensive transcriptome analysis of mouse embryonic stem cell adipogenesis unravels new processes of adipocyte development
title_sort comprehensive transcriptome analysis of mouse embryonic stem cell adipogenesis unravels new processes of adipocyte development
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2945782/
https://www.ncbi.nlm.nih.gov/pubmed/20678241
http://dx.doi.org/10.1186/gb-2010-11-8-r80
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