Sequencing bias: comparison of different protocols of MicroRNA library construction

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BACKGROUND: MicroRNAs(miRNAs) are 18-25 nt small RNAs playing critical roles in many biological processes. The majority of known miRNAs were discovered by conventional cloning and a Sanger sequencing approach. The next-generation sequencing (NGS) technologies enable in-depth characterization of the...

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Detalles Bibliográficos
Autores principales: Tian, Geng, Yin, XuYang, Luo, Hong, Xu, XiaoHong, Bolund, Lars, Zhang, XiuQing
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2946280/
https://www.ncbi.nlm.nih.gov/pubmed/20815927
http://dx.doi.org/10.1186/1472-6750-10-64
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