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Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters
Tularemia, caused by the bacterium Francisella tularensis, where F. tularensis subspecies holarctica has long been the cause of endemic disease in parts of northern Sweden. Despite this, our understanding of the natural life-cycle of the organism is still limited. During three years, we collected su...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2946586/ https://www.ncbi.nlm.nih.gov/pubmed/20885922 http://dx.doi.org/10.1155/2011/851946 |
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author | Broman, T. Thelaus, J. Andersson, A.-C. Bäckman, S. Wikström, P. Larsson, E. Granberg, M. Karlsson, L. Bäck, E. Eliasson, H. Mattsson, R. Sjöstedt, A. Forsman, M. |
author_facet | Broman, T. Thelaus, J. Andersson, A.-C. Bäckman, S. Wikström, P. Larsson, E. Granberg, M. Karlsson, L. Bäck, E. Eliasson, H. Mattsson, R. Sjöstedt, A. Forsman, M. |
author_sort | Broman, T. |
collection | PubMed |
description | Tularemia, caused by the bacterium Francisella tularensis, where F. tularensis subspecies holarctica has long been the cause of endemic disease in parts of northern Sweden. Despite this, our understanding of the natural life-cycle of the organism is still limited. During three years, we collected surface water samples (n = 341) and sediment samples (n = 245) in two areas in Sweden with endemic tularemia. Real-time PCR screening demonstrated the presence of F. tularenis lpnA sequences in 108 (32%) and 48 (20%) of the samples, respectively. The 16S rRNA sequences from those samples all grouped to the species F. tularensis. Analysis of the FtM19InDel region of lpnA-positive samples from selected sampling points confirmed the presence of F. tularensis subspecies holarctica-specific sequences. These sequences were detected in water sampled during both outbreak and nonoutbreak years. Our results indicate that diverse F. tularensis-like organisms, including F. tularensis subsp. holarctica, persist in natural waters and sediments in the investigated areas with endemic tularemia. |
format | Text |
id | pubmed-2946586 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-29465862010-09-30 Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters Broman, T. Thelaus, J. Andersson, A.-C. Bäckman, S. Wikström, P. Larsson, E. Granberg, M. Karlsson, L. Bäck, E. Eliasson, H. Mattsson, R. Sjöstedt, A. Forsman, M. Int J Microbiol Research Article Tularemia, caused by the bacterium Francisella tularensis, where F. tularensis subspecies holarctica has long been the cause of endemic disease in parts of northern Sweden. Despite this, our understanding of the natural life-cycle of the organism is still limited. During three years, we collected surface water samples (n = 341) and sediment samples (n = 245) in two areas in Sweden with endemic tularemia. Real-time PCR screening demonstrated the presence of F. tularenis lpnA sequences in 108 (32%) and 48 (20%) of the samples, respectively. The 16S rRNA sequences from those samples all grouped to the species F. tularensis. Analysis of the FtM19InDel region of lpnA-positive samples from selected sampling points confirmed the presence of F. tularensis subspecies holarctica-specific sequences. These sequences were detected in water sampled during both outbreak and nonoutbreak years. Our results indicate that diverse F. tularensis-like organisms, including F. tularensis subsp. holarctica, persist in natural waters and sediments in the investigated areas with endemic tularemia. Hindawi Publishing Corporation 2011 2010-09-08 /pmc/articles/PMC2946586/ /pubmed/20885922 http://dx.doi.org/10.1155/2011/851946 Text en Copyright © 2011 T. Broman et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Broman, T. Thelaus, J. Andersson, A.-C. Bäckman, S. Wikström, P. Larsson, E. Granberg, M. Karlsson, L. Bäck, E. Eliasson, H. Mattsson, R. Sjöstedt, A. Forsman, M. Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters |
title | Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters |
title_full | Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters |
title_fullStr | Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters |
title_full_unstemmed | Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters |
title_short | Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters |
title_sort | molecular detection of persistent francisella tularensis subspecies holarctica in natural waters |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2946586/ https://www.ncbi.nlm.nih.gov/pubmed/20885922 http://dx.doi.org/10.1155/2011/851946 |
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