Inflammatory dendritic cells—not basophils—are necessary and sufficient for induction of Th2 immunity to inhaled house dust mite allergen

It is unclear how Th2 immunity is induced in response to allergens like house dust mite (HDM). Here, we show that HDM inhalation leads to the TLR4/MyD88-dependent recruitment of IL-4 competent basophils and eosinophils, and of inflammatory DCs to the draining mediastinal nodes. Depletion of basophil...

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Autores principales: Hammad, Hamida, Plantinga, Maud, Deswarte, Kim, Pouliot, Philippe, Willart, Monique A.M., Kool, Mirjam, Muskens, Femke, Lambrecht, Bart N.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2947072/
https://www.ncbi.nlm.nih.gov/pubmed/20819925
http://dx.doi.org/10.1084/jem.20101563
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author Hammad, Hamida
Plantinga, Maud
Deswarte, Kim
Pouliot, Philippe
Willart, Monique A.M.
Kool, Mirjam
Muskens, Femke
Lambrecht, Bart N.
author_facet Hammad, Hamida
Plantinga, Maud
Deswarte, Kim
Pouliot, Philippe
Willart, Monique A.M.
Kool, Mirjam
Muskens, Femke
Lambrecht, Bart N.
author_sort Hammad, Hamida
collection PubMed
description It is unclear how Th2 immunity is induced in response to allergens like house dust mite (HDM). Here, we show that HDM inhalation leads to the TLR4/MyD88-dependent recruitment of IL-4 competent basophils and eosinophils, and of inflammatory DCs to the draining mediastinal nodes. Depletion of basophils only partially reduced Th2 immunity, and depletion of eosinophils had no effect on the Th2 response. Basophils did not take up inhaled antigen, present it to T cells, or express antigen presentation machinery, whereas a population of FceRI(+) DCs readily did. Inflammatory DCs were necessary and sufficient for induction of Th2 immunity and features of asthma, whereas basophils were not required. We favor a model whereby DCs initiate and basophils amplify Th2 immunity to HDM allergen.
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spelling pubmed-29470722011-03-27 Inflammatory dendritic cells—not basophils—are necessary and sufficient for induction of Th2 immunity to inhaled house dust mite allergen Hammad, Hamida Plantinga, Maud Deswarte, Kim Pouliot, Philippe Willart, Monique A.M. Kool, Mirjam Muskens, Femke Lambrecht, Bart N. J Exp Med Article It is unclear how Th2 immunity is induced in response to allergens like house dust mite (HDM). Here, we show that HDM inhalation leads to the TLR4/MyD88-dependent recruitment of IL-4 competent basophils and eosinophils, and of inflammatory DCs to the draining mediastinal nodes. Depletion of basophils only partially reduced Th2 immunity, and depletion of eosinophils had no effect on the Th2 response. Basophils did not take up inhaled antigen, present it to T cells, or express antigen presentation machinery, whereas a population of FceRI(+) DCs readily did. Inflammatory DCs were necessary and sufficient for induction of Th2 immunity and features of asthma, whereas basophils were not required. We favor a model whereby DCs initiate and basophils amplify Th2 immunity to HDM allergen. The Rockefeller University Press 2010-09-27 /pmc/articles/PMC2947072/ /pubmed/20819925 http://dx.doi.org/10.1084/jem.20101563 Text en © 2010 Hammad et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Article
Hammad, Hamida
Plantinga, Maud
Deswarte, Kim
Pouliot, Philippe
Willart, Monique A.M.
Kool, Mirjam
Muskens, Femke
Lambrecht, Bart N.
Inflammatory dendritic cells—not basophils—are necessary and sufficient for induction of Th2 immunity to inhaled house dust mite allergen
title Inflammatory dendritic cells—not basophils—are necessary and sufficient for induction of Th2 immunity to inhaled house dust mite allergen
title_full Inflammatory dendritic cells—not basophils—are necessary and sufficient for induction of Th2 immunity to inhaled house dust mite allergen
title_fullStr Inflammatory dendritic cells—not basophils—are necessary and sufficient for induction of Th2 immunity to inhaled house dust mite allergen
title_full_unstemmed Inflammatory dendritic cells—not basophils—are necessary and sufficient for induction of Th2 immunity to inhaled house dust mite allergen
title_short Inflammatory dendritic cells—not basophils—are necessary and sufficient for induction of Th2 immunity to inhaled house dust mite allergen
title_sort inflammatory dendritic cells—not basophils—are necessary and sufficient for induction of th2 immunity to inhaled house dust mite allergen
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2947072/
https://www.ncbi.nlm.nih.gov/pubmed/20819925
http://dx.doi.org/10.1084/jem.20101563
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