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Phosphorylated P68 RNA Helicase Activates Snail1 Transcription by Promoting HDAC1 Dissociation from the Snail1 Promoter

The nuclear p68 RNA helicase is a prototypical member of the DEAD box family of RNA helicases. P68 RNA helicase has been implicated in cell proliferation and early organ development and maturation. However, the functional role of p68 RNA helicase in these biological processes at the molecular level...

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Autores principales: Carter, Christie L., Lin, Chunru, Liu, Chia-yi, Yang, Liuqing, Liu, Zhi-Ren
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2948064/
https://www.ncbi.nlm.nih.gov/pubmed/20676135
http://dx.doi.org/10.1038/onc.2010.276
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author Carter, Christie L.
Lin, Chunru
Liu, Chia-yi
Yang, Liuqing
Liu, Zhi-Ren
author_facet Carter, Christie L.
Lin, Chunru
Liu, Chia-yi
Yang, Liuqing
Liu, Zhi-Ren
author_sort Carter, Christie L.
collection PubMed
description The nuclear p68 RNA helicase is a prototypical member of the DEAD box family of RNA helicases. P68 RNA helicase has been implicated in cell proliferation and early organ development and maturation. However, the functional role of p68 RNA helicase in these biological processes at the molecular level is not well understood. We previously reported that tyrosine phosphorylation of p68 RNA helicase mediates the effects of PDGF in induction of EMT by promoting β-catenin nuclear translocation (Yang et.al. Cell 127:139-155 2006). Here we report that phosphorylation of p68 RNA helicase at Y593 up-regulates transcription of the Snail1 gene. The phosphorylated p68 activates transcription of the Snail1 gene by promoting HDAC1 dissociation from the Snail1 promoter. Our results showed that p68 interacted with the nuclear remodeling and deacetylation complex MBD3:Mi-2/NuRD. Thus, our data suggested that a DEAD box RNA unwindase can potentially regulate gene expression by functioning as a protein ‘displacer’ to modulate protein-protein interactions at the chromatin remodeling complex.
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spelling pubmed-29480642011-03-30 Phosphorylated P68 RNA Helicase Activates Snail1 Transcription by Promoting HDAC1 Dissociation from the Snail1 Promoter Carter, Christie L. Lin, Chunru Liu, Chia-yi Yang, Liuqing Liu, Zhi-Ren Oncogene Article The nuclear p68 RNA helicase is a prototypical member of the DEAD box family of RNA helicases. P68 RNA helicase has been implicated in cell proliferation and early organ development and maturation. However, the functional role of p68 RNA helicase in these biological processes at the molecular level is not well understood. We previously reported that tyrosine phosphorylation of p68 RNA helicase mediates the effects of PDGF in induction of EMT by promoting β-catenin nuclear translocation (Yang et.al. Cell 127:139-155 2006). Here we report that phosphorylation of p68 RNA helicase at Y593 up-regulates transcription of the Snail1 gene. The phosphorylated p68 activates transcription of the Snail1 gene by promoting HDAC1 dissociation from the Snail1 promoter. Our results showed that p68 interacted with the nuclear remodeling and deacetylation complex MBD3:Mi-2/NuRD. Thus, our data suggested that a DEAD box RNA unwindase can potentially regulate gene expression by functioning as a protein ‘displacer’ to modulate protein-protein interactions at the chromatin remodeling complex. 2010-08-02 2010-09-30 /pmc/articles/PMC2948064/ /pubmed/20676135 http://dx.doi.org/10.1038/onc.2010.276 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Carter, Christie L.
Lin, Chunru
Liu, Chia-yi
Yang, Liuqing
Liu, Zhi-Ren
Phosphorylated P68 RNA Helicase Activates Snail1 Transcription by Promoting HDAC1 Dissociation from the Snail1 Promoter
title Phosphorylated P68 RNA Helicase Activates Snail1 Transcription by Promoting HDAC1 Dissociation from the Snail1 Promoter
title_full Phosphorylated P68 RNA Helicase Activates Snail1 Transcription by Promoting HDAC1 Dissociation from the Snail1 Promoter
title_fullStr Phosphorylated P68 RNA Helicase Activates Snail1 Transcription by Promoting HDAC1 Dissociation from the Snail1 Promoter
title_full_unstemmed Phosphorylated P68 RNA Helicase Activates Snail1 Transcription by Promoting HDAC1 Dissociation from the Snail1 Promoter
title_short Phosphorylated P68 RNA Helicase Activates Snail1 Transcription by Promoting HDAC1 Dissociation from the Snail1 Promoter
title_sort phosphorylated p68 rna helicase activates snail1 transcription by promoting hdac1 dissociation from the snail1 promoter
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2948064/
https://www.ncbi.nlm.nih.gov/pubmed/20676135
http://dx.doi.org/10.1038/onc.2010.276
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