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Phosphorylated P68 RNA Helicase Activates Snail1 Transcription by Promoting HDAC1 Dissociation from the Snail1 Promoter
The nuclear p68 RNA helicase is a prototypical member of the DEAD box family of RNA helicases. P68 RNA helicase has been implicated in cell proliferation and early organ development and maturation. However, the functional role of p68 RNA helicase in these biological processes at the molecular level...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2948064/ https://www.ncbi.nlm.nih.gov/pubmed/20676135 http://dx.doi.org/10.1038/onc.2010.276 |
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author | Carter, Christie L. Lin, Chunru Liu, Chia-yi Yang, Liuqing Liu, Zhi-Ren |
author_facet | Carter, Christie L. Lin, Chunru Liu, Chia-yi Yang, Liuqing Liu, Zhi-Ren |
author_sort | Carter, Christie L. |
collection | PubMed |
description | The nuclear p68 RNA helicase is a prototypical member of the DEAD box family of RNA helicases. P68 RNA helicase has been implicated in cell proliferation and early organ development and maturation. However, the functional role of p68 RNA helicase in these biological processes at the molecular level is not well understood. We previously reported that tyrosine phosphorylation of p68 RNA helicase mediates the effects of PDGF in induction of EMT by promoting β-catenin nuclear translocation (Yang et.al. Cell 127:139-155 2006). Here we report that phosphorylation of p68 RNA helicase at Y593 up-regulates transcription of the Snail1 gene. The phosphorylated p68 activates transcription of the Snail1 gene by promoting HDAC1 dissociation from the Snail1 promoter. Our results showed that p68 interacted with the nuclear remodeling and deacetylation complex MBD3:Mi-2/NuRD. Thus, our data suggested that a DEAD box RNA unwindase can potentially regulate gene expression by functioning as a protein ‘displacer’ to modulate protein-protein interactions at the chromatin remodeling complex. |
format | Text |
id | pubmed-2948064 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
record_format | MEDLINE/PubMed |
spelling | pubmed-29480642011-03-30 Phosphorylated P68 RNA Helicase Activates Snail1 Transcription by Promoting HDAC1 Dissociation from the Snail1 Promoter Carter, Christie L. Lin, Chunru Liu, Chia-yi Yang, Liuqing Liu, Zhi-Ren Oncogene Article The nuclear p68 RNA helicase is a prototypical member of the DEAD box family of RNA helicases. P68 RNA helicase has been implicated in cell proliferation and early organ development and maturation. However, the functional role of p68 RNA helicase in these biological processes at the molecular level is not well understood. We previously reported that tyrosine phosphorylation of p68 RNA helicase mediates the effects of PDGF in induction of EMT by promoting β-catenin nuclear translocation (Yang et.al. Cell 127:139-155 2006). Here we report that phosphorylation of p68 RNA helicase at Y593 up-regulates transcription of the Snail1 gene. The phosphorylated p68 activates transcription of the Snail1 gene by promoting HDAC1 dissociation from the Snail1 promoter. Our results showed that p68 interacted with the nuclear remodeling and deacetylation complex MBD3:Mi-2/NuRD. Thus, our data suggested that a DEAD box RNA unwindase can potentially regulate gene expression by functioning as a protein ‘displacer’ to modulate protein-protein interactions at the chromatin remodeling complex. 2010-08-02 2010-09-30 /pmc/articles/PMC2948064/ /pubmed/20676135 http://dx.doi.org/10.1038/onc.2010.276 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Carter, Christie L. Lin, Chunru Liu, Chia-yi Yang, Liuqing Liu, Zhi-Ren Phosphorylated P68 RNA Helicase Activates Snail1 Transcription by Promoting HDAC1 Dissociation from the Snail1 Promoter |
title | Phosphorylated P68 RNA Helicase Activates Snail1 Transcription by Promoting HDAC1 Dissociation from the Snail1 Promoter |
title_full | Phosphorylated P68 RNA Helicase Activates Snail1 Transcription by Promoting HDAC1 Dissociation from the Snail1 Promoter |
title_fullStr | Phosphorylated P68 RNA Helicase Activates Snail1 Transcription by Promoting HDAC1 Dissociation from the Snail1 Promoter |
title_full_unstemmed | Phosphorylated P68 RNA Helicase Activates Snail1 Transcription by Promoting HDAC1 Dissociation from the Snail1 Promoter |
title_short | Phosphorylated P68 RNA Helicase Activates Snail1 Transcription by Promoting HDAC1 Dissociation from the Snail1 Promoter |
title_sort | phosphorylated p68 rna helicase activates snail1 transcription by promoting hdac1 dissociation from the snail1 promoter |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2948064/ https://www.ncbi.nlm.nih.gov/pubmed/20676135 http://dx.doi.org/10.1038/onc.2010.276 |
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