A genetic system to assess in vivo the functions of histones and histone modifications in higher eukaryotes

Despite the fundamental role of canonical histones in nucleosome structure, there is no experimental system for higher eukaryotes in which basic questions about histone function can be directly addressed. We developed a new genetic tool for Drosophila melanogaster in which the canonical histone comp...

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Detalles Bibliográficos
Autores principales: Günesdogan, Ufuk, Jäckle, Herbert, Herzig, Alf
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2010
Materias:
Scientific Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2948182/
https://www.ncbi.nlm.nih.gov/pubmed/20814422
http://dx.doi.org/10.1038/embor.2010.124
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author Günesdogan, Ufuk
Jäckle, Herbert
Herzig, Alf
author_facet Günesdogan, Ufuk
Jäckle, Herbert
Herzig, Alf
author_sort Günesdogan, Ufuk
collection PubMed
description Despite the fundamental role of canonical histones in nucleosome structure, there is no experimental system for higher eukaryotes in which basic questions about histone function can be directly addressed. We developed a new genetic tool for Drosophila melanogaster in which the canonical histone complement can be replaced with multiple copies of experimentally modified histone transgenes. This new histone-replacement system provides a well-defined and direct cellular assay system for histone function with which to critically test models in chromatin biology dealing with chromatin assembly, variant histone functions and the biological significance of distinct histone modifications in a multicellular organism.
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spelling pubmed-29481822010-10-25 A genetic system to assess in vivo the functions of histones and histone modifications in higher eukaryotes Günesdogan, Ufuk Jäckle, Herbert Herzig, Alf EMBO Rep Scientific Reports Despite the fundamental role of canonical histones in nucleosome structure, there is no experimental system for higher eukaryotes in which basic questions about histone function can be directly addressed. We developed a new genetic tool for Drosophila melanogaster in which the canonical histone complement can be replaced with multiple copies of experimentally modified histone transgenes. This new histone-replacement system provides a well-defined and direct cellular assay system for histone function with which to critically test models in chromatin biology dealing with chromatin assembly, variant histone functions and the biological significance of distinct histone modifications in a multicellular organism. Nature Publishing Group 2010-10 2010-09-03 /pmc/articles/PMC2948182/ /pubmed/20814422 http://dx.doi.org/10.1038/embor.2010.124 Text en Copyright © 2010, European Molecular Biology Organization http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial No Derivative Works 3.0 Unported License, which permits distribution and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation or the creation of derivative works without specific permission.
spellingShingle Scientific Reports
Günesdogan, Ufuk
Jäckle, Herbert
Herzig, Alf
A genetic system to assess in vivo the functions of histones and histone modifications in higher eukaryotes
title A genetic system to assess in vivo the functions of histones and histone modifications in higher eukaryotes
title_full A genetic system to assess in vivo the functions of histones and histone modifications in higher eukaryotes
title_fullStr A genetic system to assess in vivo the functions of histones and histone modifications in higher eukaryotes
title_full_unstemmed A genetic system to assess in vivo the functions of histones and histone modifications in higher eukaryotes
title_short A genetic system to assess in vivo the functions of histones and histone modifications in higher eukaryotes
title_sort genetic system to assess in vivo the functions of histones and histone modifications in higher eukaryotes
topic Scientific Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2948182/
https://www.ncbi.nlm.nih.gov/pubmed/20814422
http://dx.doi.org/10.1038/embor.2010.124
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