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Yeast proteomics and protein microarrays
Our understanding of biological processes as well as human diseases has improved greatly thanks to studies on model organisms such as yeast. The power of scientific approaches with yeast lies in its relatively simple genome, its facile classical and molecular genetics, as well as the evolutionary co...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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Elsevier B.V.
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2949546/ https://www.ncbi.nlm.nih.gov/pubmed/20728591 http://dx.doi.org/10.1016/j.jprot.2010.08.003 |
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author | Chen, Rui Snyder, Michael |
author_facet | Chen, Rui Snyder, Michael |
author_sort | Chen, Rui |
collection | PubMed |
description | Our understanding of biological processes as well as human diseases has improved greatly thanks to studies on model organisms such as yeast. The power of scientific approaches with yeast lies in its relatively simple genome, its facile classical and molecular genetics, as well as the evolutionary conservation of many basic biological mechanisms. However, even in this simple model organism, systems biology studies, especially proteomic studies had been an intimidating task. During the past decade, powerful high-throughput technologies in proteomic research have been developed for yeast including protein microarray technology. The protein microarray technology allows the interrogation of protein–protein, protein–DNA, protein–small molecule interaction networks as well as post-translational modification networks in a large-scale, high-throughput manner. With this technology, many groundbreaking findings have been established in studies with the budding yeast Saccharomyces cerevisiae, most of which could have been unachievable with traditional approaches. Discovery of these networks has profound impact on explicating biological processes with a proteomic point of view, which may lead to a better understanding of normal biological phenomena as well as various human diseases. |
format | Text |
id | pubmed-2949546 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-29495462011-10-10 Yeast proteomics and protein microarrays Chen, Rui Snyder, Michael J Proteomics Article Our understanding of biological processes as well as human diseases has improved greatly thanks to studies on model organisms such as yeast. The power of scientific approaches with yeast lies in its relatively simple genome, its facile classical and molecular genetics, as well as the evolutionary conservation of many basic biological mechanisms. However, even in this simple model organism, systems biology studies, especially proteomic studies had been an intimidating task. During the past decade, powerful high-throughput technologies in proteomic research have been developed for yeast including protein microarray technology. The protein microarray technology allows the interrogation of protein–protein, protein–DNA, protein–small molecule interaction networks as well as post-translational modification networks in a large-scale, high-throughput manner. With this technology, many groundbreaking findings have been established in studies with the budding yeast Saccharomyces cerevisiae, most of which could have been unachievable with traditional approaches. Discovery of these networks has profound impact on explicating biological processes with a proteomic point of view, which may lead to a better understanding of normal biological phenomena as well as various human diseases. Elsevier B.V. 2010-10-10 2010-08-20 /pmc/articles/PMC2949546/ /pubmed/20728591 http://dx.doi.org/10.1016/j.jprot.2010.08.003 Text en Copyright © 2010 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Chen, Rui Snyder, Michael Yeast proteomics and protein microarrays |
title | Yeast proteomics and protein microarrays |
title_full | Yeast proteomics and protein microarrays |
title_fullStr | Yeast proteomics and protein microarrays |
title_full_unstemmed | Yeast proteomics and protein microarrays |
title_short | Yeast proteomics and protein microarrays |
title_sort | yeast proteomics and protein microarrays |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2949546/ https://www.ncbi.nlm.nih.gov/pubmed/20728591 http://dx.doi.org/10.1016/j.jprot.2010.08.003 |
work_keys_str_mv | AT chenrui yeastproteomicsandproteinmicroarrays AT snydermichael yeastproteomicsandproteinmicroarrays |