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Red Ginseng Extract Facilitates the Early Differentiation of Human Embryonic Stem Cells into Mesendoderm Lineage

Human embryonic stem cells (hESCs) have capacities to self-renew and differentiate into all cell types in vitro. Red ginseng (RG) is known to have a wide range of pharmacological effects in vivo; however, the reports on its effects on hESCs are few. In this paper, we tried to demonstrate the effects...

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Autores principales: Kim, Yoon Young, Ku, Seung-Yup, Rosenwaks, Zev, Liu, Hung Ching, Oh, Sun Kyung, Moon, Shin Yong, Choi, Young Min
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2949591/
https://www.ncbi.nlm.nih.gov/pubmed/20924497
http://dx.doi.org/10.1155/2011/167376
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author Kim, Yoon Young
Ku, Seung-Yup
Rosenwaks, Zev
Liu, Hung Ching
Oh, Sun Kyung
Moon, Shin Yong
Choi, Young Min
author_facet Kim, Yoon Young
Ku, Seung-Yup
Rosenwaks, Zev
Liu, Hung Ching
Oh, Sun Kyung
Moon, Shin Yong
Choi, Young Min
author_sort Kim, Yoon Young
collection PubMed
description Human embryonic stem cells (hESCs) have capacities to self-renew and differentiate into all cell types in vitro. Red ginseng (RG) is known to have a wide range of pharmacological effects in vivo; however, the reports on its effects on hESCs are few. In this paper, we tried to demonstrate the effects of RG on the proliferation and differentiation of hESCs. Undifferentiated hESCs, embryoid bodies (EBs), and hESC-derived cardiac progenitors (CPs) were treated with RG extract at 0.125, 0.25, and 0.5 mg/mL. After treatment of undifferentiated hESCs from day 2 to day 6 of culture, BrdU labeling showed that RG treatment increased the proliferation of hESCs, and the expression of Oct4 and Nanog was increased in RG-treated group. To find out the effects of RG on early differentiation stage cells, EBs were treated with RG extract for 10 days and attached for further differentiation. Immunostaining for three germ layer markers showed that RG treatment increased the expressions of Brachyury and HNF3β on EBs. Also, RG treatment increased the expression of Brachyury in early-stage and of Nkx2.5 in late-stage hESC-derived CPs. These results demonstrate facilitating effects of RG extract on the proliferation and early differentiation of hESC.
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spelling pubmed-29495912010-10-05 Red Ginseng Extract Facilitates the Early Differentiation of Human Embryonic Stem Cells into Mesendoderm Lineage Kim, Yoon Young Ku, Seung-Yup Rosenwaks, Zev Liu, Hung Ching Oh, Sun Kyung Moon, Shin Yong Choi, Young Min Evid Based Complement Alternat Med Research Article Human embryonic stem cells (hESCs) have capacities to self-renew and differentiate into all cell types in vitro. Red ginseng (RG) is known to have a wide range of pharmacological effects in vivo; however, the reports on its effects on hESCs are few. In this paper, we tried to demonstrate the effects of RG on the proliferation and differentiation of hESCs. Undifferentiated hESCs, embryoid bodies (EBs), and hESC-derived cardiac progenitors (CPs) were treated with RG extract at 0.125, 0.25, and 0.5 mg/mL. After treatment of undifferentiated hESCs from day 2 to day 6 of culture, BrdU labeling showed that RG treatment increased the proliferation of hESCs, and the expression of Oct4 and Nanog was increased in RG-treated group. To find out the effects of RG on early differentiation stage cells, EBs were treated with RG extract for 10 days and attached for further differentiation. Immunostaining for three germ layer markers showed that RG treatment increased the expressions of Brachyury and HNF3β on EBs. Also, RG treatment increased the expression of Brachyury in early-stage and of Nkx2.5 in late-stage hESC-derived CPs. These results demonstrate facilitating effects of RG extract on the proliferation and early differentiation of hESC. Hindawi Publishing Corporation 2011 2010-09-01 /pmc/articles/PMC2949591/ /pubmed/20924497 http://dx.doi.org/10.1155/2011/167376 Text en Copyright © 2011 Yoon Young Kim et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kim, Yoon Young
Ku, Seung-Yup
Rosenwaks, Zev
Liu, Hung Ching
Oh, Sun Kyung
Moon, Shin Yong
Choi, Young Min
Red Ginseng Extract Facilitates the Early Differentiation of Human Embryonic Stem Cells into Mesendoderm Lineage
title Red Ginseng Extract Facilitates the Early Differentiation of Human Embryonic Stem Cells into Mesendoderm Lineage
title_full Red Ginseng Extract Facilitates the Early Differentiation of Human Embryonic Stem Cells into Mesendoderm Lineage
title_fullStr Red Ginseng Extract Facilitates the Early Differentiation of Human Embryonic Stem Cells into Mesendoderm Lineage
title_full_unstemmed Red Ginseng Extract Facilitates the Early Differentiation of Human Embryonic Stem Cells into Mesendoderm Lineage
title_short Red Ginseng Extract Facilitates the Early Differentiation of Human Embryonic Stem Cells into Mesendoderm Lineage
title_sort red ginseng extract facilitates the early differentiation of human embryonic stem cells into mesendoderm lineage
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2949591/
https://www.ncbi.nlm.nih.gov/pubmed/20924497
http://dx.doi.org/10.1155/2011/167376
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