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Comparison of culture and qPCR for the detection of Pseudomonas aeruginosa in not chronically infected cystic fibrosis patients

BACKGROUND: Pseudomonas aeruginosa is the major respiratory pathogen causing severe lung infections among CF patients, leading to high morbidity and mortality. Once infection is established, early antibiotic treatment is able to postpone the transition to chronic lung infection. In order to optimize...

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Autores principales: Deschaght, Pieter, Schelstraete, Petra, Lopes dos Santos Santiago, Guido, Van Simaey, Leen, Haerynck, Filomeen, Van daele, Sabine, De Wachter, Elke, Malfroot, Anne, Lebecque, Patrick, Knoop, Christiane, Casimir, Georges, Boboli, Hedwige, Pierart, Frédéric, Desager, Kristine, Vaneechoutte, Mario, De Baets, Frans
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2949703/
https://www.ncbi.nlm.nih.gov/pubmed/20868481
http://dx.doi.org/10.1186/1471-2180-10-245
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author Deschaght, Pieter
Schelstraete, Petra
Lopes dos Santos Santiago, Guido
Van Simaey, Leen
Haerynck, Filomeen
Van daele, Sabine
De Wachter, Elke
Malfroot, Anne
Lebecque, Patrick
Knoop, Christiane
Casimir, Georges
Boboli, Hedwige
Pierart, Frédéric
Desager, Kristine
Vaneechoutte, Mario
De Baets, Frans
author_facet Deschaght, Pieter
Schelstraete, Petra
Lopes dos Santos Santiago, Guido
Van Simaey, Leen
Haerynck, Filomeen
Van daele, Sabine
De Wachter, Elke
Malfroot, Anne
Lebecque, Patrick
Knoop, Christiane
Casimir, Georges
Boboli, Hedwige
Pierart, Frédéric
Desager, Kristine
Vaneechoutte, Mario
De Baets, Frans
author_sort Deschaght, Pieter
collection PubMed
description BACKGROUND: Pseudomonas aeruginosa is the major respiratory pathogen causing severe lung infections among CF patients, leading to high morbidity and mortality. Once infection is established, early antibiotic treatment is able to postpone the transition to chronic lung infection. In order to optimize the early detection, we compared the sensitivity of microbiological culture and quantitative PCR (qPCR) for the detection of P. aeruginosa in respiratory samples of not chronically infected CF patients. RESULTS: In this national study, we followed CF patients during periods between 1 to 15 months. For a total of 852 samples, 729 (86%) remained P. aeruginosa negative by both culture and qPCR, whereas 89 samples (10%) were positive by both culture and qPCR. Twenty-six samples were negative by culture but positive by qPCR, and 10 samples were positive by culture but remained negative by qPCR. Five of the 26 patients with a culture negative, qPCR positive sample became later P. aeruginosa positive both by culture and qPCR. CONCLUSION: Based on the results of this study, it can be concluded that qPCR may have a predictive value for impending P. aeruginosa infection for only a limited number of patients.
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spelling pubmed-29497032010-10-06 Comparison of culture and qPCR for the detection of Pseudomonas aeruginosa in not chronically infected cystic fibrosis patients Deschaght, Pieter Schelstraete, Petra Lopes dos Santos Santiago, Guido Van Simaey, Leen Haerynck, Filomeen Van daele, Sabine De Wachter, Elke Malfroot, Anne Lebecque, Patrick Knoop, Christiane Casimir, Georges Boboli, Hedwige Pierart, Frédéric Desager, Kristine Vaneechoutte, Mario De Baets, Frans BMC Microbiol Research Article BACKGROUND: Pseudomonas aeruginosa is the major respiratory pathogen causing severe lung infections among CF patients, leading to high morbidity and mortality. Once infection is established, early antibiotic treatment is able to postpone the transition to chronic lung infection. In order to optimize the early detection, we compared the sensitivity of microbiological culture and quantitative PCR (qPCR) for the detection of P. aeruginosa in respiratory samples of not chronically infected CF patients. RESULTS: In this national study, we followed CF patients during periods between 1 to 15 months. For a total of 852 samples, 729 (86%) remained P. aeruginosa negative by both culture and qPCR, whereas 89 samples (10%) were positive by both culture and qPCR. Twenty-six samples were negative by culture but positive by qPCR, and 10 samples were positive by culture but remained negative by qPCR. Five of the 26 patients with a culture negative, qPCR positive sample became later P. aeruginosa positive both by culture and qPCR. CONCLUSION: Based on the results of this study, it can be concluded that qPCR may have a predictive value for impending P. aeruginosa infection for only a limited number of patients. BioMed Central 2010-09-24 /pmc/articles/PMC2949703/ /pubmed/20868481 http://dx.doi.org/10.1186/1471-2180-10-245 Text en Copyright ©2010 Deschaght et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Deschaght, Pieter
Schelstraete, Petra
Lopes dos Santos Santiago, Guido
Van Simaey, Leen
Haerynck, Filomeen
Van daele, Sabine
De Wachter, Elke
Malfroot, Anne
Lebecque, Patrick
Knoop, Christiane
Casimir, Georges
Boboli, Hedwige
Pierart, Frédéric
Desager, Kristine
Vaneechoutte, Mario
De Baets, Frans
Comparison of culture and qPCR for the detection of Pseudomonas aeruginosa in not chronically infected cystic fibrosis patients
title Comparison of culture and qPCR for the detection of Pseudomonas aeruginosa in not chronically infected cystic fibrosis patients
title_full Comparison of culture and qPCR for the detection of Pseudomonas aeruginosa in not chronically infected cystic fibrosis patients
title_fullStr Comparison of culture and qPCR for the detection of Pseudomonas aeruginosa in not chronically infected cystic fibrosis patients
title_full_unstemmed Comparison of culture and qPCR for the detection of Pseudomonas aeruginosa in not chronically infected cystic fibrosis patients
title_short Comparison of culture and qPCR for the detection of Pseudomonas aeruginosa in not chronically infected cystic fibrosis patients
title_sort comparison of culture and qpcr for the detection of pseudomonas aeruginosa in not chronically infected cystic fibrosis patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2949703/
https://www.ncbi.nlm.nih.gov/pubmed/20868481
http://dx.doi.org/10.1186/1471-2180-10-245
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