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Characterising B cell numbers and memory B cells in HIV infected and uninfected Malawian adults

BACKGROUND: Untreated human immunodeficiency virus (HIV) disease disrupts B cell populations causing reduced memory and reduced naïve resting B cells leading to increases in specific co-infections and impaired responses to vaccines. To what extent antiretroviral treatment reverses these changes in a...

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Autores principales: Longwe, Herbert, Gordon, Stephen, Malamba, Rose, French, Neil
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2949763/
https://www.ncbi.nlm.nih.gov/pubmed/20860822
http://dx.doi.org/10.1186/1471-2334-10-280
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author Longwe, Herbert
Gordon, Stephen
Malamba, Rose
French, Neil
author_facet Longwe, Herbert
Gordon, Stephen
Malamba, Rose
French, Neil
author_sort Longwe, Herbert
collection PubMed
description BACKGROUND: Untreated human immunodeficiency virus (HIV) disease disrupts B cell populations causing reduced memory and reduced naïve resting B cells leading to increases in specific co-infections and impaired responses to vaccines. To what extent antiretroviral treatment reverses these changes in an African population is uncertain. METHODS: A cross-sectional study was performed. We recruited HIV-uninfected and HIV-infected Malawian adults both on and off antiretroviral therapy attending the Queen Elizabeth Central hospital in Malawi. Using flow cytometry, we enumerated B cells and characterized memory B cells and compared these measurements by the different recruitment groups. RESULTS: Overall 64 participants were recruited - 20 HIV uninfected (HIV-), 30 HIV infected ART naïve (HIV+N) and 14 HIV-infected ART treated (HIV+T). ART treatment had been taken for a median of 33 months (Range 12-60 months). Compared to HIV- the HIV+N adults had low absolute number of naïve resting B cells (111 vs. 180 cells/μl p = 0.008); reduced memory B cells (27 vs. 51 cells/μl p = 0.0008). The HIV+T adults had B-cell numbers similar to HIV- except for memory B cells that remained significantly lower (30 vs. 51 cells/μl p = 0.02). In the HIV+N group we did not find an association between CD4 count and B cell numbers. CONCLUSIONS: HIV infected Malawian adults have abnormal B-cell numbers. Individuals treated with ART show a return to normal in B-cell numbers but a persistent deficit in the memory subset is noted. This has important implications for long term susceptibility to co-infections and should be evaluated further in a larger cohort study.
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spelling pubmed-29497632010-10-06 Characterising B cell numbers and memory B cells in HIV infected and uninfected Malawian adults Longwe, Herbert Gordon, Stephen Malamba, Rose French, Neil BMC Infect Dis Research Article BACKGROUND: Untreated human immunodeficiency virus (HIV) disease disrupts B cell populations causing reduced memory and reduced naïve resting B cells leading to increases in specific co-infections and impaired responses to vaccines. To what extent antiretroviral treatment reverses these changes in an African population is uncertain. METHODS: A cross-sectional study was performed. We recruited HIV-uninfected and HIV-infected Malawian adults both on and off antiretroviral therapy attending the Queen Elizabeth Central hospital in Malawi. Using flow cytometry, we enumerated B cells and characterized memory B cells and compared these measurements by the different recruitment groups. RESULTS: Overall 64 participants were recruited - 20 HIV uninfected (HIV-), 30 HIV infected ART naïve (HIV+N) and 14 HIV-infected ART treated (HIV+T). ART treatment had been taken for a median of 33 months (Range 12-60 months). Compared to HIV- the HIV+N adults had low absolute number of naïve resting B cells (111 vs. 180 cells/μl p = 0.008); reduced memory B cells (27 vs. 51 cells/μl p = 0.0008). The HIV+T adults had B-cell numbers similar to HIV- except for memory B cells that remained significantly lower (30 vs. 51 cells/μl p = 0.02). In the HIV+N group we did not find an association between CD4 count and B cell numbers. CONCLUSIONS: HIV infected Malawian adults have abnormal B-cell numbers. Individuals treated with ART show a return to normal in B-cell numbers but a persistent deficit in the memory subset is noted. This has important implications for long term susceptibility to co-infections and should be evaluated further in a larger cohort study. BioMed Central 2010-09-22 /pmc/articles/PMC2949763/ /pubmed/20860822 http://dx.doi.org/10.1186/1471-2334-10-280 Text en Copyright ©2010 Longwe et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Longwe, Herbert
Gordon, Stephen
Malamba, Rose
French, Neil
Characterising B cell numbers and memory B cells in HIV infected and uninfected Malawian adults
title Characterising B cell numbers and memory B cells in HIV infected and uninfected Malawian adults
title_full Characterising B cell numbers and memory B cells in HIV infected and uninfected Malawian adults
title_fullStr Characterising B cell numbers and memory B cells in HIV infected and uninfected Malawian adults
title_full_unstemmed Characterising B cell numbers and memory B cells in HIV infected and uninfected Malawian adults
title_short Characterising B cell numbers and memory B cells in HIV infected and uninfected Malawian adults
title_sort characterising b cell numbers and memory b cells in hiv infected and uninfected malawian adults
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2949763/
https://www.ncbi.nlm.nih.gov/pubmed/20860822
http://dx.doi.org/10.1186/1471-2334-10-280
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