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A case-controlled validation study of a blood-based seven-gene biomarker panel for colorectal cancer in Malaysia
BACKGROUND: Colorectal cancer (CRC) screening is key to CRC prevention and mortality reduction, but patient compliance with CRC screening is low. We previously reported a blood-based test for CRC that utilizes a seven-gene panel of biomarkers. The test is currently utilized clinically in North Ameri...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2949823/ https://www.ncbi.nlm.nih.gov/pubmed/20846378 http://dx.doi.org/10.1186/1756-9966-29-128 |
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author | Yip, Kok-Thye Das, Prashanta K Suria, David Lim, Chun-Ren Ng, Guey-Hooi Liew, Choong-Chin |
author_facet | Yip, Kok-Thye Das, Prashanta K Suria, David Lim, Chun-Ren Ng, Guey-Hooi Liew, Choong-Chin |
author_sort | Yip, Kok-Thye |
collection | PubMed |
description | BACKGROUND: Colorectal cancer (CRC) screening is key to CRC prevention and mortality reduction, but patient compliance with CRC screening is low. We previously reported a blood-based test for CRC that utilizes a seven-gene panel of biomarkers. The test is currently utilized clinically in North America for CRC risk stratification in the average-risk North American population in order to improve screening compliance and to enhance clinical decision making. METHODS: In this study, conducted in Malaysia, we evaluated the seven-gene biomarker panel validated in a North American population using blood samples collected from local patients. The panel employs quantitative RT-PCR (qRT-PCR) to analyze gene expression of the seven biomarkers (ANXA3, CLEC4D, TNFAIP6, LMNB1, PRRG4, VNN1 and IL2RB) that are differentially expressed in CRC patients as compared with controls. Blood samples from 210 patients (99 CRC and 111 controls) were collected, and total blood RNA was isolated and subjected to quantitative RT-PCR and data analysis. RESULTS: The logistic regression analysis of seven-gene panel has an area under the curve (AUC) of 0.76 (95% confidence interval: 0.70 to 0.82), 77% specificity, 61% sensitivity and 70% accuracy, comparable to the data obtained from the North American investigation of the same biomarker panel. CONCLUSIONS: Our results independently confirm the results of the study conducted in North America and demonstrate the ability of the seven biomarker panel to discriminate CRC from controls in blood samples drawn from a Malaysian population. |
format | Text |
id | pubmed-2949823 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-29498232010-10-06 A case-controlled validation study of a blood-based seven-gene biomarker panel for colorectal cancer in Malaysia Yip, Kok-Thye Das, Prashanta K Suria, David Lim, Chun-Ren Ng, Guey-Hooi Liew, Choong-Chin J Exp Clin Cancer Res Research BACKGROUND: Colorectal cancer (CRC) screening is key to CRC prevention and mortality reduction, but patient compliance with CRC screening is low. We previously reported a blood-based test for CRC that utilizes a seven-gene panel of biomarkers. The test is currently utilized clinically in North America for CRC risk stratification in the average-risk North American population in order to improve screening compliance and to enhance clinical decision making. METHODS: In this study, conducted in Malaysia, we evaluated the seven-gene biomarker panel validated in a North American population using blood samples collected from local patients. The panel employs quantitative RT-PCR (qRT-PCR) to analyze gene expression of the seven biomarkers (ANXA3, CLEC4D, TNFAIP6, LMNB1, PRRG4, VNN1 and IL2RB) that are differentially expressed in CRC patients as compared with controls. Blood samples from 210 patients (99 CRC and 111 controls) were collected, and total blood RNA was isolated and subjected to quantitative RT-PCR and data analysis. RESULTS: The logistic regression analysis of seven-gene panel has an area under the curve (AUC) of 0.76 (95% confidence interval: 0.70 to 0.82), 77% specificity, 61% sensitivity and 70% accuracy, comparable to the data obtained from the North American investigation of the same biomarker panel. CONCLUSIONS: Our results independently confirm the results of the study conducted in North America and demonstrate the ability of the seven biomarker panel to discriminate CRC from controls in blood samples drawn from a Malaysian population. BioMed Central 2010-09-16 /pmc/articles/PMC2949823/ /pubmed/20846378 http://dx.doi.org/10.1186/1756-9966-29-128 Text en Copyright ©2010 Yip et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Yip, Kok-Thye Das, Prashanta K Suria, David Lim, Chun-Ren Ng, Guey-Hooi Liew, Choong-Chin A case-controlled validation study of a blood-based seven-gene biomarker panel for colorectal cancer in Malaysia |
title | A case-controlled validation study of a blood-based seven-gene biomarker panel for colorectal cancer in Malaysia |
title_full | A case-controlled validation study of a blood-based seven-gene biomarker panel for colorectal cancer in Malaysia |
title_fullStr | A case-controlled validation study of a blood-based seven-gene biomarker panel for colorectal cancer in Malaysia |
title_full_unstemmed | A case-controlled validation study of a blood-based seven-gene biomarker panel for colorectal cancer in Malaysia |
title_short | A case-controlled validation study of a blood-based seven-gene biomarker panel for colorectal cancer in Malaysia |
title_sort | case-controlled validation study of a blood-based seven-gene biomarker panel for colorectal cancer in malaysia |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2949823/ https://www.ncbi.nlm.nih.gov/pubmed/20846378 http://dx.doi.org/10.1186/1756-9966-29-128 |
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